Non-canonical GABAergic Pathways in the Visual System

视觉系统中的非典型 GABA 能通路

• 批准号: 10202616
• 负责人: Tiffany M. Schmidt
• 金额: $ 45.39万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10202616
• 关键词: Anatomy; Area; Axon; Behavior; Brain; Brain region; Cells; Characteristics; Conscious; Data; Electrophysiology (science); Evaluation; Eye; Ganglion Cell Layer; Glutamate Decarboxylase; Glutamates; Human; IGL@ gene cluster; Immunohistochemistry; Knock-out; Lead; Light; Literature; Maps; Modeling; Mus; Neurotransmitters; Optic Nerve; Organism; Oryctolagus cuniculus; Pathway interactions; Peptides; Photosensitivity; Physiology; Population; Pupil light reflex; Research; Retina; Retinal Ganglion Cells; Role; Signal Transduction; Sleep; Subconscious; Testing; Time; Viral; Visual; Visual Perception; Visual system structure; Work; base; behavior influence; circadian; experimental study; gamma-Aminobutyric Acid; ganglion cell; immunoreactivity; melanopsin; mouse genetics; neurotransmission; neurotransmitter release; patch clamp; postsynaptic; prevent

PROJECT SUMMARY Light is a profoundly important regulator of physiology and behavior across a wide range of organisms. Light information is relayed from the retina to the brain via glutamate released from retinal ganglion cells (RGCs) at more than 40 brain regions, with some RGCs also co-releasing peptide transmitters. Despite the prevailing dogma that all RGCs release glutamate, there have been hints that some RGCs may be GABAergic. However, the identity and function of these potentially GABAergic RGCs is completely unknown. Our recent findings indicate that a subset of the melanopsin-expressing, intrinsically photosensitive (ip)RGCs are GABAergic. This proposal will test the overarching hypothesis that GABA release from the melanopsin- expressing, intrinsically photosensitive (ip)RGCs influences non-image forming behaviors. The aims of this proposal will define the types and targets of GABAergic RGCs and their role in visual behavior using a combination of mouse genetics, channelrhodopsin-based circuit tracing, anatomy, patch clamp electrophysiology, and behavior. In addition to mapping the identity and function of GABAergic RGCs, these studies will open up new areas of research into the role of inhibitory circuits in light-driven behavior and physiology.

项目摘要 光是广泛生物体的生理和行为的非常重要的调节剂。 光信息通过视网膜神经节细胞从视网膜传递到大脑。 （RGC）在40多个大脑区域，一些RGC还共同发出肽发射器。尽管有 所有RGC释放谷氨酸的盛行教条，有一些提示某些RGC可能是GABA能。 但是，这些潜在的GABA能RGC的身份和功能是完全未知的。我们最近 调查结果表明，表达黑色素蛋白的子集，本质上具有光敏性（IP）RGC是 加巴能。该提案将检验总体假设，即GABA从黑色素蛋白 - 表达本质的光敏（IP）RGC会影响非图像形成行为。这个目的 建议将定义GABA能RGC的类型和目标及其在视觉行为中的作用 小鼠遗传学，基于通道旋转的电路跟踪，解剖学，斑块夹的组合 电生理学和行为。除了映射GABA能RGC的身份和功能外，这些 研究将为抑制电路在轻度驱动行为中的作用和 生理。