Sympathetic regulation of inflammation from sleep fragmentation

项目摘要 原发睡眠异常(失眠、倒班和阻塞性睡眠呼吸暂停)会促进炎症 并与心血管和代谢性疾病的发展有关。然而， 人们对这些关系背后的机制知之甚少。这项拟议的研究检查了荷尔蒙 睡眠碎片(SF)炎症反应的药理学基础机制 在小鼠身上的方法。我们实验室之前由R15资助的研究检测了交感神经的影响 系统(SNS)在对失眠的炎症反应时被激活。在此续订申请中， 下丘脑-垂体-肾上腺(HPA)轴的激活在调节急性和慢性炎症中发挥作用 将对慢性SF进行调查。目标1将研究糖皮质激素是否在SF诱导的高血压发病中起作用 炎症和小胶质细胞的激活，这是神经炎症的重要因素。具体来说，老鼠 将接受肾上腺切除、假肾上腺切除或肾上腺切除并用外源性 糖皮质激素，然后接受急性和慢性SF。促炎和抗炎基因和蛋白 表达将分别使用RT-PCR和多路Luminex蛋白质阵列进行评估。 免疫细胞化学将被用来评估小胶质细胞的激活。这些发现将通过以下方式进行验证 药物抑制糖皮质激素的产生和与糖皮质激素受体的结合。而不是一个 经典抗炎作用，预测糖皮质激素将启动SF诱导的炎症反应。目标2 将检查糖皮质激素释放的时间进程和对急性和慢性的促炎反应 科幻小说。综上所述，拟议的研究将评估肾上腺皮质反应在 调节失眠时的炎症，为本科生提供独特的训练机会 对内分泌学、免疫学和神经科学方面的生物医学研究感兴趣。

Project Summary Primary sleep abnormalities (insomnia, shift work, and obstructive sleep apnea) promote an inflammatory environment and are associated with the development of cardiovascular and metabolic disease. However, the mechanisms underlying these relationships are poorly understood. The proposed research examines hormonal mechanisms that underlie the inflammatory response to sleep fragmentation (SF) using pharmacological approaches in mice. Previous R15-funded research in our lab examined the effect of sympathetic nervous system (SNS) activation upon inflammatory responses to sleep loss. In this renewal application, the role that activation of the hypothalamic-pituitary-adrenal (HPA) axis plays in modulating inflammation from acute and chronic SF will be investigated. Aim 1 will examine whether glucocorticoids mediate the onset of SF-induced inflammation and the activation of microglia, an important contributor to neuroinflammation. Specifically, mice will be adrenalectomized, sham-adrenalectomized, or adrenalectomized and rescued with exogenous glucocorticoids and then subjected to acute and chronic SF. Pro- and antiinflammatory gene and protein expression will be assessed using RT-PCR and multiplex Luminex protein arrays, respectively. Immunocytochemistry will be used to assess microglia activation. These findings will be validated through pharmacological inhibition of glucocorticoid production and binding to glucocorticoid receptors. Instead of a classic anti-inflammatory effect, it is predicted that glucocorticoids will prime SF-induced inflammation. Aim 2 will examine the time course of glucocorticoid release and pro-inflammatory responses to acute and chronic SF. Taken together, the proposed research will assess the importance of the adrenocortical response in mediating inflammation during sleep loss, and provide unique training opportunities for undergraduates interested in conducting biomedical research in endocrinology, immunology, and the neurosciences.