Systems Serology Core

系统血清学核心

• 批准号: 10203487
• 负责人: Galit Alter
• 金额: $ 39.61万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-12 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10203487
• 关键词: Address; Adult; Affect; Antibodies; Antibody Response; Area; Autologous; Benchmarking; Biological Assay; Biophysics; Birth; Cells; Collection; Data; Development; Engineering; Ensure; Environment; Fingerprint; Generations; Goals; Human; Human Resources; Humoral Immunities; Immune; Immune response; Immune system; Immunity; Immunologics; Infant; Infant Health; Infection; Infrastructure; Institutes; Intervention; Investigation; Knowledge; Laboratories; Leadership; Life; Maternal antibody; Maternally-Acquired Immunity; Monitor; Nature; Neonatal; Newborn Infant; Pregnancy; Protocols documentation; Reagent; Research; Resources; Role; Running; Sampling; Serology; Standardization; System; Technical Expertise; Technology; Therapeutic; Training; Vaccination; Vaccines; Work; conditioning; high standard; improved; in utero; laboratory experience; maternal vaccination; neonatal immunity; neonate; new technology; next generation; novel; programs; response; success; synergism; tool

CORE 1 – SYSTEMS SEROLOGY CORE – ABSTRACT Accumulating data points to dramatic immunologic changes during pregnancy and early life. However, how these immune perturbations alter the immune response to vaccination or to infection have yet to be fully defined. Under Core 1, in depth comprehensive characterization will be performed on the humoral immune response to improve our understanding of the mechanisms by which the immune system changes throughout pregnancy, how these changes affect responses to vaccines and how infants benefit from maternal immunity and in turn respond to vaccines. Specifically, Core 1 will exploit and evolve Systems Serology to broadly and deeply probe the nature of the humoral immune response in a sample sparing manner, via the development of multiplexed-antibody profiling approaches. Additionally Core 1 will develop lower-throughput functional assays to probe the interaction between antibodies and host-innate immune cells, in a fully autologous fashion, to define the overall impact of altered antibody and cellular state in pregnancy and early life. Thus Core 1 will deeply interrogate and define changes in humoral immunity throughout pregnancy, at birth, and throughout early infant life. The Core leader will provide all the expertise, infrastructure, reagents, personnel, and training, to ensure the efficient and successful analysis and deployment of this technology under Projects 1 & 2, as well as support in generating data for Projects 2 & 4. Thus the main role of this Core is to provide the leadership and the technical expertise to ensure that antibody assays are run in a standard fashion, that novel technologies evolve from this Core to support the needs of the overall Consortium, and that the Consortium is kept abreast of the latest developments in assessing antibody responses.

核心1 -系统血清学核心-摘要 越来越多的数据表明，在怀孕和生命早期，免疫系统发生了巨大的变化。然而，这些 免疫扰动改变对疫苗接种或感染的免疫应答还没有完全确定。下 核心1、深入全面的表征将对体液免疫应答进行提升 我们对免疫系统在整个怀孕过程中变化的机制的理解， 变化影响对疫苗的反应，以及婴儿如何从母体免疫中受益， 疫苗。具体来说，核心1将利用和发展系统血清学，以广泛和深入地探讨自然 体液免疫反应的样品节省的方式，通过开发的多重抗体 剖析方法。此外，核心1将开发低通量功能测定，以探测相互作用 抗体和宿主先天免疫细胞之间的关系，以完全自体的方式，以确定 在怀孕和生命早期改变抗体和细胞状态。因此，核心1将深入询问和定义 在整个妊娠期、出生时和整个婴儿早期的体液免疫变化。核心领导 将提供所有的专业知识，基础设施，试剂，人员和培训，以确保高效， 在项目1和2下成功分析和部署这项技术，并支持生成 项目2和项目4的数据。因此，该核心的主要作用是提供领导和技术专长， 为了确保抗体检测以标准方式运行，新技术从本核心发展到 支持整个联合体的需求，并确保联合体随时了解最新发展 评估抗体反应。