Method validation and comparison for the detection of mycotoxins in novel animal

机会编号PA-13-244宾夕法尼亚大学- 新型动物饲料和组织中霉菌毒素的检测 项目概要/摘要 PADLS新博尔顿中心毒理学实验室建议调查适应能力， 验证CharmROSA-M阅读器用于分析优先真菌毒素（黄曲霉毒素，DON， 玉米赤霉烯酮、T-2/HT-2、伏马菌素和赭曲霉毒素），并比较 它的使用到其他容易获得的方法。如果Charm ROSA-M阅读器可用于 分析狗的食物，牛饲料，猪饲料，他们将提供一个简单的方法，最低限度的训练， 多个实验室的工作人员，以便在发生 疑似动物饲料污染事件分析方法的验证和比较 在动物饲料和宠物食品中感兴趣的其它真菌毒素（15-AcDON，青霉烯A，罗克福汀C， 也将被执行。 由于最近的历史污染事件涉及黄曲霉毒素的狗食品和频繁的数字 在涉及宠物食品的调查中，我们建议首先使用这种基质类型。我们 第二种基质类型将是牛饲料，因为动物和人类的健康风险可能会发生， 霉菌毒素污染意味着无论是牛肉或奶牛，特别是霉菌毒素，可以通过 变成牛奶基于广泛使用玉米为基础的饲料猪，因为这个物种往往是其中 对于那些对霉菌毒素最敏感的动物，我们选择了猪饲料作为我们的第三种饲料基质。 在该项目的最后阶段，将制定分析选定真菌毒素的方法 并在犬、牛和猪的组织和生物体液样品中进行了验证， 全面调查受污染的动物饲料事件，特别是这些物种。 在我们的实验室完成对每个分析物和基质的验证后，将发送样品 供其他7个Vet-LIRN合作实验室验证。该项目的最终产品将是一个 使用Charm ROSA-M分析饲料中优先霉菌毒素的标准化方法SOP 饲料中其他霉菌毒素分析的标准操作规程和选定的 使用LC/MS/MS检测动物样品中的真菌毒素。 1

Opportunity #PA-13-244 University of Pennsylvania - Method Validation and Comparison for the Detection of Mycotoxins in Novel Animal Feeds and Tissues PROJECT SUMMARY/ABSTRACT The PADLS New Bolton Center Toxicology Laboratory proposes to investigate the ability to adapt and validate the use of the Charm ROSA-M reader for the analysis of priority mycotoxins (aflatoxins, DON, zearalenone, T-2/HT-2, fumonisins, and ochratoxin) in complex animal feeds and pet foods and compare its use to other readily-available methods. If the Charm ROSA-M readers can be validated for the analysis of dog food, cattle feed, and swine feed, they will provide an easy method for minimally-trained personnel at multiple laboratories to quickly screen large volumes of samples in the event of a suspected animal feed contamination incident. Validation and comparison of methods for the analysis of additional mycotoxins of interest in animal feeds and pet foods (15-AcDON, penitrem A, roquefortine C, and moniliformin) will also be performed. Due to recent historical contamination events involving aflatoxins in dog food and the frequent number of investigations involving pet foods in general, we propose working with this matrix type first. Our second matrix type will be cattle feed due to the animal and human health risks that could occur due to mycotoxin contamination meant for either beef or dairy cattle, particularly the mycotoxins that can pass into milk. Based on the wide use of corn-based feeds for swine and because this species is often among those most sensitive to mycotoxins, we have selected swine feed as our third feed matrix. During the final phases of the project, methods for the analysis of selected mycotoxins will be developed and validated in tissues and biological fluids samples from dogs, cattle, and swine to best facilitate complete investigations of contaminated animal feed events, particularly for these species. After completing the validation for each analyte and matrix in our laboratory, samples will be sent out for validation to 7 other collaborating Vet-LIRN laboratories. The final products for this project will be a standardized method SOP for the analysis of priority mycotoxins in feeds using the Charm ROSA-M reader and harmonized method SOPs for the analysis of additional mycotoxins in feeds and selected mycotoxins in animal samples using LC/MS/MS. 1