Method validation and comparison for the detection of mycotoxins in novel animal

机会#PA-13-244宾夕法尼亚大学-方法验证和比较 新型动物饲料和组织中真菌毒素的检测 项目摘要/摘要 PADLS新博尔顿中心毒理学实验室建议调查适应和 验证使用CHAME ROSA-M读取器分析优先真菌毒素(黄曲霉毒素、DON、 玉米赤霉烯酮、T-2/HT-2、伏马菌素和赭曲霉毒素)在复合动物饲料和宠物食品中的含量并进行比较 它的用途可以推广到其他现成的方法。如果Charm ROSA-M阅读器可以被验证为 分析狗饲料，牛饲料和猪饲料，他们将提供一个简单的方法，最低限度的培训 多个实验室的人员在发生 疑似动物饲料污染事件。几种分析方法的验证与比较 动物饲料和宠物食品中的其他感兴趣的真菌毒素(15-AcDON，penitrem A，roquefortin C， 和念珠菌素)也将进行。 由于最近发生的涉及狗粮中黄曲霉毒素的历史污染事件和频繁出现的 对于一般涉及宠物食品的调查，我们建议首先处理这种矩阵类型。我们的 第二种基质类型将是牛饲料，因为由于以下原因可能会发生动物和人类健康风险 霉菌毒素污染是指对牛肉或奶牛，特别是可以通过的霉菌毒素 变成牛奶。基于以玉米为基础的饲料对猪的广泛使用，并且因为这种物种经常是 对于那些对真菌毒素最敏感的动物，我们选择了猪饲料作为第三种饲料基质。 在该项目的最后阶段，将开发分析选定真菌毒素的方法。 并在狗、牛和猪的组织和生物液样本中进行验证，以最大限度地促进 对受污染的动物饲料事件进行全面调查，特别是对这些物种。 在我们实验室完成每个分析物和基质的验证后，样品将被送出 用于向其他7个合作的Vet-LIRN实验室进行验证。该项目的最终产品将是 使用CHAME ROSA-M分析饲料中优先霉菌毒素的标准化方法SOP 分析饲料和精选饲料中其他真菌毒素的读者和协调方法标准操作规程 动物样品中真菌毒素的LC/MS/MS分析 1

Opportunity #PA-13-244 University of Pennsylvania - Method Validation and Comparison for the Detection of Mycotoxins in Novel Animal Feeds and Tissues PROJECT SUMMARY/ABSTRACT The PADLS New Bolton Center Toxicology Laboratory proposes to investigate the ability to adapt and validate the use of the Charm ROSA-M reader for the analysis of priority mycotoxins (aflatoxins, DON, zearalenone, T-2/HT-2, fumonisins, and ochratoxin) in complex animal feeds and pet foods and compare its use to other readily-available methods. If the Charm ROSA-M readers can be validated for the analysis of dog food, cattle feed, and swine feed, they will provide an easy method for minimally-trained personnel at multiple laboratories to quickly screen large volumes of samples in the event of a suspected animal feed contamination incident. Validation and comparison of methods for the analysis of additional mycotoxins of interest in animal feeds and pet foods (15-AcDON, penitrem A, roquefortine C, and moniliformin) will also be performed. Due to recent historical contamination events involving aflatoxins in dog food and the frequent number of investigations involving pet foods in general, we propose working with this matrix type first. Our second matrix type will be cattle feed due to the animal and human health risks that could occur due to mycotoxin contamination meant for either beef or dairy cattle, particularly the mycotoxins that can pass into milk. Based on the wide use of corn-based feeds for swine and because this species is often among those most sensitive to mycotoxins, we have selected swine feed as our third feed matrix. During the final phases of the project, methods for the analysis of selected mycotoxins will be developed and validated in tissues and biological fluids samples from dogs, cattle, and swine to best facilitate complete investigations of contaminated animal feed events, particularly for these species. After completing the validation for each analyte and matrix in our laboratory, samples will be sent out for validation to 7 other collaborating Vet-LIRN laboratories. The final products for this project will be a standardized method SOP for the analysis of priority mycotoxins in feeds using the Charm ROSA-M reader and harmonized method SOPs for the analysis of additional mycotoxins in feeds and selected mycotoxins in animal samples using LC/MS/MS. 1