NYU Pediatric Obesity, Metabolism and Kidney Cohort Center

项目摘要 新型冠状病毒大流行（COVID-19）在很大程度上使儿童幸免于难，需要医疗的儿童相对较少 在乎一个小型病例系列未能揭示对COVID-19肺炎母亲分娩结局的影响，但 没有影响可能部分与这些母亲在妊娠晚期感染有关，因为时间 众所周知，感染会对妊娠产生不良影响。一个主要的障碍，以人口为基础， 对SARS-CoV-2感染的研究一直缺乏一种非侵入性的检测病毒RNA的方法。 鼻咽拭子是侵入性的，需要个人防护设备和拭子， 有限的供应，并且可能不如唾液样本用于SARS-CoV-2检测的敏感性。研究 儿童感染后的适应性免疫也受到静脉穿刺收集的限制 用于验证抗体检测的充足血清。我们的研究团队开发了新的方法， 测量唾液中的SARS-CoV-2感染，以及对刺突蛋白的IgG和IgM抗体反应， 干血斑（DBS）中SARS-CoV-2的受体结合域。巨大的心理压力 可能在大流行期间发生在孕妇身上，无论是由于害怕感染，失业， 经济压力、心理或身体创伤或其他因素。怀孕期间的心理压力 已知会增加早产，但压力和病毒的影响还没有一起研究过。我们有 此前已获得PO许可，重新分配纽约大学儿童健康与环境项目的4年级资金 研究（CHES，UH 3 OD 23305）和哥伦比亚儿童环境健康中心（CCCEH， UH 3 OD 023290），以在队列中添加远程和重复的唾液、干血斑和毛发采集 使用Fisher BioSciences为ECHO组装的试剂盒，前瞻性招募母亲入组ECHO 程序.我们建议在明年将这项工作扩大到纽约大学CHES新登记的母亲（N=200 母亲）、CCCEH（N=20位母亲）以及密歇根州儿童健康研究档案（MARCH， UH 3 OD 23285，N=20位母亲）。我们还希望继续在已入组的NYU CHES中收集数据 （截至2020年3月1日，N=340名孕妇）和CCCEH（截至2020年3月1日，N=40名孕妇） 妈妈们所有SARS-CoV-2检测的数据将通过图表摘要进行补充。

PROJECT SUMMARY The novel coronavirus pandemic (COVID-19) has largely spared children, with relatively few requiring medical care. A small case series failed to reveal effects on birth outcomes in mothers with COVID-19 pneumonia, yet the absence of effects may in part relate to these mothers having been infected in third trimester, as the timing of infection is well known to influence adverse effects in pregnancy. A major barrier to population-based studies of SARS-CoV-2 infection has been the lack of a noninvasive method to detect viral RNA. Nasopharyngeal swabs are invasive, require personal protective equipment and swabs which have been in limited supply, and may be less sensitive than salivary samples for SARS-CoV-2 detection. Studies of adaptive immunity following infection in children have also been limited by the need for venipuncture to collect sufficient serum for validated testing of antibody. Our research team has developed novel methods for measuring SARS-CoV-2 infection in saliva, as well as IgG and IgM antibody responses to the spike protein and the receptor binding domain of SARS-CoV-2 in dried blood spots (DBS). Substantial psychosocial stress is likely to have occurred in pregnant women during the pandemic, whether due to fear of infection, job loss, economic stress, psychological or physical trauma, or other factors. Psychological stress during pregnancy is known to increase prematurity, yet stress and viral effects have not been examined together as yet. We had previously received PO permission to reallocate Year 4 funds in the NYU Children’s Health and Environment Study (CHES, UH3OD23305) and the Columbia Center for Children’s Environmental Health (CCCEH, UH3OD023290) to add remote and repeated saliva, dried blood spot, and hair collection in cohorts prospectively enrolling mothers into ECHO, using kits assembled by Fisher BioSciences for the ECHO program. We propose to expand this effort over the next year to newly enrolled mothers in NYU CHES (N=200 mothers), CCCEH (N=20 mothers) as well as the Michigan Archive for Research in Children’s Health (MARCH, UH3OD23285, N=20 mothers). We also wish to continue data collection in already enrolled NYU CHES (N=340 pregnant women as of March 1, 2020) and CCCEH (N=40 pregnant women as of March 1, 2020) mothers. The data will be supplemented by chart abstraction for all SARS-CoV-2 testing.