Vet-LIRN Network Capacity-Buildinng Projects - Implementation of automated liquid handler for high throughput MALDI-TOF and PCR testing

Vet-LIRN 网络能力建设项目 - 实施自动化液体处理机以进行高通量 MALDI-TOF 和 PCR 测试

• 批准号: 10215140
• 负责人: Bruce W Brodersen
• 金额: $ 5.82万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-20 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10215140
• 关键词: Vet; LIRN; Network; Capacity; Buildinng

Project Summary The Nebraska Veterinary Diagnostic Center (VDC) is a full service veterinary diagnostic laboratory. The VDC is fully accredited by the American Association of Veterinary Laboratory Diagnosticians, is a National Animal Health Laboratory Network (NAHLN) Level 1 laboratory and is a certificated laboratory for National Poultry Improvement Plan testing. The laboratory processes over 300,000 specimens annually, conducting a variety of tests including ELISA, serum virus neutralization, AGID, hemagglutination, polymerase chain reaction, bacterial isolation from field specimens and identification, MALDI‐ TOF based identification and characterization of bacterial isolates, antimicrobial susceptibility testing, fluorescent antibody tests, immunohistochemistry, histopathology, and necropsy exams. In order to be more efficient in processing this large caseload, it is important that the laboratory is equipped with state‐of‐the‐art instrumentation and automation for high throughput testing to maintain capacity. This is particularly true in the event of facing high volume testing while participating with a response of a foreign animal disease outbreak or emergency response. The proposal is to purchase an instrument which would be used to automate and enhance capacity for repetitive tasks particularly for set‐up of MALDI TOF plates, and a variety of molecular or other tests with low volume and precise liquid handling requirements

项目摘要 内布拉斯加州兽医诊断中心（VDC）是一个提供全方位服务的兽医诊断实验室。的 VDC是由美国兽医实验室诊断师协会完全认可，是一个 国家动物卫生实验室网络（NAHLN）1级实验室，是一个认证的实验室 国家家禽改良计划测试该实验室处理超过30万个样本 每年进行多种检测，包括ELISA、血清病毒中和、AGID、 血凝、聚合酶链反应、现场标本细菌分离和鉴定， 基于MALDI-TOF的细菌分离株鉴定和表征、抗菌药物敏感性 检测、荧光抗体检测、免疫组织化学、组织病理学和尸检。在 为了更有效地处理这一大量案件，实验室必须 配备最先进的仪器和自动化设备，用于高通量测试， 容量这在面临大量测试的情况下尤其如此，同时参与 应对外国动物疾病暴发或紧急情况。建议是购买一个 这些工具将用于使重复性任务自动化并提高其能力， MALDI TOF板的设置以及各种分子或其他测试，使用小体积和精确的液体 处理要求