Vet-LIRN Network Capacity Building for SARS-CoV-2: equipment for analysis of NGS library and data

项目摘要 伊利诺伊大学(UI)兽医诊断实验室(VDL)是一项全面服务，AAVLD认证，所有 物种，参考实验室。UI VDL是VET-LIRN的成员。截至2023年2月17日，UI VDL已测试 3287个SARS-CoV-2样本和13个不同动物园的350个动物园动物样本检测呈阳性。下一步- 世代测序在SARS-CoV-2病例调查中的兽医诊断中是非常有用的 可能受到多种因素的影响，包括测序方法的选择、测序文库 质量、病毒基因组数量、样本质量、样本池和生物信息学分析。新设备 对于NGS文库和大数据的分析将大大提高我们的SARS-CoV-2检测能力和 Vet-LIRN协调常规病例诊断和监测的服务质量，从而提高Vet-LIRN 用于SARS-CoV-2检测的网络实验室。

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference laboratory. UI VDL is a member of the VET-LIRN. As of Feb 17, 2023, UI VDL has tested 3,287 samples for SARS-CoV-2 and 350 samples of zoo animals in 13 different zoos were tested positive. Next- generation sequencing (NGS) is very useful in case investigation of SARS-CoV-2 in the veterinary diagnostic laboratory and could be affected by many factors including selection of sequencing methods, sequencing library quality, amount of viral genome, sample quality, sample pooling, and bioinformatics analysis. New equipment for analyzing both NGS library and big data will significantly increase our SARS-CoV-2 testing capacity and service quality for routine case diagnosis and surveillance coordinated by Vet-LIRN, thus enhancing Vet-LIRN network laboratory for SARS-CoV-2 testing.