Project 2: Serologic and molecular determinants of COVID-19 severity and immune protection
项目 2:COVID-19 严重程度和免疫保护的血清学和分子决定因素
基本信息
- 批准号:10222411
- 负责人:
- 金额:$ 81.82万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-18 至 2022-08-31
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAgeAnimalsAntibodiesAntibody ResponseAntibody-Dependent EnhancementBindingBiological AssayBloodCOVID-19COVID-19 pandemicChildClinicalCommon ColdDataData SetDevelopmentDiagnosticDiseaseDisease OutcomeElderlyEnzyme-Linked Immunosorbent AssayEpitopesExposure toFamilyFrequenciesFutureGeneral PopulationGenesGeneticGenetic PolymorphismGenetic TranscriptionGoalsHouseholdHumanImmuneImmune responseImmunityImmunoglobulin AImmunoglobulin GImmunoglobulin IsotypesImmunoglobulin MImmunologic FactorsImmunologistImmunoprecipitationIndividualInfectionInfection preventionIntegration Host FactorsKineticsKnowledgeLeadLinkLuciferasesMeasuresMediatingMiddle East Respiratory Syndrome CoronavirusMolecularMonitorMucosal Immune ResponsesMucous MembraneMutationOpen Reading FramesOutcomePathogenesisPatientsPatternPeptidesPopulationPrevalencePrevention strategyProteinsProtocols documentationPublic HealthReverse Transcriptase Polymerase Chain ReactionRiskSARS coronavirusSamplingSerologic testsSerologicalSerumSeveritiesSeverity of illnessSourceSpecificitySymptomsTestingVaccinatedVaccinesViralViral ProteinsViruscareerco-infectioncohortcomorbiditycoronavirus diseasefirst respondergene inductionhigh riskhuman coronavirusimproved outcomeinfection riskinnovationinsightmembermucosal siteneutralizing antibodynovelresponsescreeningseropositivetranscriptometranscriptome sequencingtransmission processtumor-immune system interactionsvaccine-induced immunity
项目摘要
Project 2 Summary
PROJECT 2. Serologic and molecular determinants of COVID-19 severity and immune protection.
Unresolved scientific questions remain about how the type, kinetics and duration of SARS-CoV-2 serologic
responses correlate with patient age, disease severity, protective immunity, and risk of spread to close
contacts. Our overall goal is to utilize the well-annotated STOP-COVID data sets and longitudinal samples
collected from the first-responder cohort (at high-risk for re-exposure) and family contacts (Project 1, Core B) to
advance understanding of the specific host and viral factors that underlie long-lasting and protective serologic
responses. Our three highly interactive aims include: to identify differences in virus neutralizing (VN) antibody
isotypes and their target viral proteins/peptides/epitopes; to assess cross-protective immunity versus disease
exacerbation by antibodies to common cold CoVs; and to probe, by virus-host transcriptome analysis, how
SARS-CoV titer or sequence and initial immune response at mucosal sites or blood interrelate with disease
outcomes and the above serologic responses. Aim 1: Define immunoglobulin isotypes, titer, target proteins and
viral neutralization activity of SARS-CoV-2 specific Abs and their correlations with disease severity and
protection: We will utilize VN assays (VNA) and a luciferase immunoprecipitation assay to identify VN antibody
isotype specificities and their immunodominant and unique target viral proteins/peptides/epitopes relevant to
disease severity and protection. Expected outcomes include data that delineate the temporal antibody isotypes
and viral targets to refine protocols for serologic testing that align with protective immunity. Aim 2: Determine
the impact of common cold CoVs (CCCoV) and SARS-CoV-2 specific antibodies on COVID-19 protection and
disease severity in a high-exposure risk cohort of first responders and their household contacts. Diagnostic
serum samples will be used in innovative ELISA platforms directed against unique and conserved CCCoV
peptides, and Spike pseudotyped CCCoV to assess CCCoV antibody responses. Expected outcomes include
original data on whether pre-existing CCCoV antibodies relate to COVID-19 clinical symptoms, severity or
strain specificity of SARS-CoV-2 infection (Aim 3), or re-infection risk. Aim 3. Correlate patterns of mucosal and
serologic immune responses with virus and host genetics. We will utilize a novel targeted host-SARS-CoV-2
RNA sequencing assay (CoV-DXVX) to assess whether virus titer or functional alterations in SARS-CoV-2 S1,
S2, N proteins or other ORFs impact disease presentation, progression and outcome. Host response
measured by the profile and strength of mucosal or blood immune gene induction will be correlated with the
duration, type and protective ability of these serologic responses and with the ones above. Once vaccines are
deployed to our first-responder cohort, all 3 aims will pivot to analyze how the above serologic response
profiles and host immune gene induction differ in vaccinated SARS-CoV-2 seronegative and seropositive
individuals compared with the naturally-infected individuals.
项目二总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Linda J. Saif其他文献
Mucosal and systemic isotype-specific antibody responses to bovine coronavirus structural proteins in naturally infected dairy calves.
自然感染的奶牛中对牛冠状病毒结构蛋白的粘膜和全身同种型特异性抗体反应。
- DOI:
- 发表时间:
1991 - 期刊:
- 影响因子:1
- 作者:
R. A. Heckert;Linda J. Saif;G. Myers - 通讯作者:
G. Myers
Infection and cross-protection studies of winter dysentery and calf diarrhea bovine coronavirus strains in colostrum-deprived and gnotobiotic calves.
初乳剥夺和限生犊牛中冬痢和犊牛腹泻牛冠状病毒株的感染和交叉保护研究。
- DOI:
- 发表时间:
1996 - 期刊:
- 影响因子:1
- 作者:
Z. El;H. Tsunemitsu;David R. Smith;Linda J. Saif - 通讯作者:
Linda J. Saif
Rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis
快速、简单地制备用于聚丙烯酰胺凝胶电泳分析的轮状病毒双链核糖核酸的方法
- DOI:
10.1128/jcm.14.3.273-280.1981 - 发表时间:
1981 - 期刊:
- 影响因子:9.4
- 作者:
K. Theil;M. Christine;McCLOSKEY;Linda J. Saif;Donald R. Redman;Edward H. Bohl;Dale D. Hancock;Erwin M. Kohler;Philip D. Moorhead - 通讯作者:
Philip D. Moorhead
Nongroup A rotaviruses of humans and animals.
人类和动物的非 A 组轮状病毒。
- DOI:
- 发表时间:
1994 - 期刊:
- 影响因子:0
- 作者:
Linda J. Saif;B. Jiang - 通讯作者:
B. Jiang
One Health perspectives on SARS-CoV-2 and other coronavirus threats to humans and animals
关于 SARS-CoV-2 以及其他对人类和动物构成威胁的冠状病毒的“一个健康”观点
- DOI:
10.1016/j.onehlt.2024.100788 - 发表时间:
2025-06-01 - 期刊:
- 影响因子:4.500
- 作者:
Linda J. Saif - 通讯作者:
Linda J. Saif
Linda J. Saif的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Linda J. Saif', 18)}}的其他基金
Rotavirus Reverse Genetics System to Study Viral Pathogenesis and Receptor Interactions
轮状病毒反向遗传学系统研究病毒发病机制和受体相互作用
- 批准号:
10739026 - 财政年份:2023
- 资助金额:
$ 81.82万 - 项目类别:
Project 2: Serologic and molecular determinants of COVID-19 severity and immune protection
项目 2:COVID-19 严重程度和免疫保护的血清学和分子决定因素
- 批准号:
10688394 - 财政年份:2020
- 资助金额:
$ 81.82万 - 项目类别:
The impact of vitamin A on the gut-mammary gland-secretory IgA axis during enteric viral infections
肠道病毒感染期间维生素 A 对肠道-乳腺-分泌 IgA 轴的影响
- 批准号:
10427171 - 财政年份:2018
- 资助金额:
$ 81.82万 - 项目类别:
The impact of vitamin A on the gut-mammary gland-secretory IgA axis during enteric viral infections
肠道病毒感染期间维生素 A 对肠道-乳腺-分泌 IgA 轴的影响
- 批准号:
9759974 - 财政年份:2018
- 资助金额:
$ 81.82万 - 项目类别:
The impact of vitamin A on the gut-mammary gland-secretory IgA axis during enteric viral infections
肠道病毒感染期间维生素 A 对肠道-乳腺-分泌 IgA 轴的影响
- 批准号:
9913564 - 财政年份:2018
- 资助金额:
$ 81.82万 - 项目类别:
Lactogenic immunity/probiotics: Effect on neonatal gut immunity
泌乳免疫/益生菌:对新生儿肠道免疫的影响
- 批准号:
7656023 - 财政年份:2009
- 资助金额:
$ 81.82万 - 项目类别:
Vitamin A adjuvant to enhance gut immunity and rotavirus vaccines in neonates
增强新生儿肠道免疫力的维生素 A 佐剂和轮状病毒疫苗
- 批准号:
7880605 - 财政年份:2009
- 资助金额:
$ 81.82万 - 项目类别:
Lactogenic immunity/probiotics: Effect on neonatal gut immunity
泌乳免疫/益生菌:对新生儿肠道免疫的影响
- 批准号:
7841951 - 财政年份:2009
- 资助金额:
$ 81.82万 - 项目类别:
Vitamin A adjuvant to enhance gut immunity and rotavirus vaccines in neonates
增强新生儿肠道免疫力的维生素 A 佐剂和轮状病毒疫苗
- 批准号:
7706606 - 财政年份:2009
- 资助金额:
$ 81.82万 - 项目类别:
Lactogenic immunity/probiotics: Effect on neonatal gut immunity
泌乳免疫/益生菌:对新生儿肠道免疫的影响
- 批准号:
8090115 - 财政年份:2009
- 资助金额:
$ 81.82万 - 项目类别:
相似国自然基金
靶向递送一氧化碳调控AGE-RAGE级联反应促进糖尿病创面愈合研究
- 批准号:JCZRQN202500010
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
对香豆酸抑制AGE-RAGE-Ang-1通路改善海马血管生成障碍发挥抗阿尔兹海默病作用
- 批准号:2025JJ70209
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
AGE-RAGE通路调控慢性胰腺炎纤维化进程的作用及分子机制
- 批准号:
- 批准年份:2024
- 资助金额:0 万元
- 项目类别:面上项目
甜茶抑制AGE-RAGE通路增强突触可塑性改善小鼠抑郁样行为
- 批准号:2023JJ50274
- 批准年份:2023
- 资助金额:0.0 万元
- 项目类别:省市级项目
蒙药额尔敦-乌日勒基础方调控AGE-RAGE信号通路改善术后认知功能障碍研究
- 批准号:
- 批准年份:2022
- 资助金额:33 万元
- 项目类别:地区科学基金项目
补肾健脾祛瘀方调控AGE/RAGE信号通路在再生障碍性贫血骨髓间充质干细胞功能受损的作用与机制研究
- 批准号:
- 批准年份:2022
- 资助金额:52 万元
- 项目类别:面上项目
LncRNA GAS5在2型糖尿病动脉粥样硬化中对AGE-RAGE 信号通路上相关基因的调控作用及机制研究
- 批准号:n/a
- 批准年份:2022
- 资助金额:10.0 万元
- 项目类别:省市级项目
围绕GLP1-Arginine-AGE/RAGE轴构建探针组学方法探索大柴胡汤异病同治的效应机制
- 批准号:81973577
- 批准年份:2019
- 资助金额:55.0 万元
- 项目类别:面上项目
AGE/RAGE通路microRNA编码基因多态性与2型糖尿病并发冠心病的关联研究
- 批准号:81602908
- 批准年份:2016
- 资助金额:18.0 万元
- 项目类别:青年科学基金项目
高血糖激活滑膜AGE-RAGE-PKC轴致骨关节炎易感的机制研究
- 批准号:81501928
- 批准年份:2015
- 资助金额:18.0 万元
- 项目类别:青年科学基金项目
相似海外基金
PROTEMO: Emotional Dynamics Of Protective Policies In An Age Of Insecurity
PROTEMO:不安全时代保护政策的情绪动态
- 批准号:
10108433 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
EU-Funded
The role of dietary and blood proteins in the prevention and development of major age-related diseases
膳食和血液蛋白在预防和发展主要与年龄相关的疾病中的作用
- 批准号:
MR/X032809/1 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Fellowship
Atomic Anxiety in the New Nuclear Age: How Can Arms Control and Disarmament Reduce the Risk of Nuclear War?
新核时代的原子焦虑:军控与裁军如何降低核战争风险?
- 批准号:
MR/X034690/1 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Fellowship
Collaborative Research: Resolving the LGM ventilation age conundrum: New radiocarbon records from high sedimentation rate sites in the deep western Pacific
合作研究:解决LGM通风年龄难题:西太平洋深部高沉降率地点的新放射性碳记录
- 批准号:
2341426 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Continuing Grant
Collaborative Research: Resolving the LGM ventilation age conundrum: New radiocarbon records from high sedimentation rate sites in the deep western Pacific
合作研究:解决LGM通风年龄难题:西太平洋深部高沉降率地点的新放射性碳记录
- 批准号:
2341424 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Continuing Grant
Doctoral Dissertation Research: Effects of age of acquisition in emerging sign languages
博士论文研究:新兴手语习得年龄的影响
- 批准号:
2335955 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Standard Grant
The economics of (mis)information in the age of social media
社交媒体时代(错误)信息的经济学
- 批准号:
DP240103257 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Discovery Projects
How age & sex impact the transcriptional control of mammalian muscle growth
你多大
- 批准号:
DP240100408 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Discovery Projects
Supporting teachers and teaching in the age of Artificial Intelligence
支持人工智能时代的教师和教学
- 批准号:
DP240100111 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Discovery Projects
Enhancing Wahkohtowin (Kinship beyond the immediate family) Community-based models of care to reach and support Indigenous and racialized women of reproductive age and pregnant women in Canada for the prevention of congenital syphilis
加强 Wahkohtowin(直系亲属以外的亲属关系)以社区为基础的护理模式,以接触和支持加拿大的土著和种族育龄妇女以及孕妇,预防先天梅毒
- 批准号:
502786 - 财政年份:2024
- 资助金额:
$ 81.82万 - 项目类别:
Directed Grant














{{item.name}}会员




