An Ultra-fast Cooling Device for Vitrification and Cryopreservation of Cells and Tissues

用于细胞和组织玻璃化和冷冻保存的超快速冷却装置

基本信息

批准号：
10221208
负责人：
Xu Han
金额：
$ 31.33万
依托单位：
CRYOCRATE, LLC
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-16 至 2021-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10221208
关键词：
Affect Age related macular degeneration Animals Behavioral Biological Biological Markers Biological Sciences Biomedical Engineering Biotechnology Cells Clinical Clinical Research Cornea Corneal Endothelium Cryopreservation Cryopreserved Cell Cryopreserved Tissue Crystallization Cytometry Data Development Device or Instrument Development Devices Dimensions Electrophysiology (science)Endothelial Cells Endothelium Engineering Excision Eye Banks Goals Health Human Ice In Vitro Keratoplasty Liquid substance Manufacturer Name Measurement Measures Methods Mission Modeling Nitrogen Notification Oryctolagus cuniculus Outcome Patients Performance Phase Process Production Regenerative Medicine Research Research Institute Retina Retinal Diseases Retinal Pigments Safety Sampling Savings Sterility System Technology Testing Thinness Tissue Donors Tissue Engineering Tissue Transplantation Tissues Transplantation Validation Vision Vision research Work base cell water clinical practice clinically relevant cryogenics design experience in vivo meetings novel operation phase 1 study pre-clinical preservation pressure prototype regenerative research and development retina transplantation scaffold simulation tool transplant model user-friendly

项目摘要

Summary The mission of CryoCrate LLC (www.CryoCrate.com) is to develop new platform cryopreservation technologies for applications in life science and clinical practice. With recent rapid advances in cell-based tissue engineering and regenerative medicine, as well as continued development of transplantation technologies, there has always been an urgent need for clinically practical methods and efficient devices for cryopreservation of large stocks of bioartificial and donor tissues. In the Phase I period of this project, we successfully established a novel ultra-fast cooling method (106K/min on cellular level) for small tissues. In the Phase II period, we will: 1) design an upgraded device system that achieves the same efficiency for cryopreservation of the whole corneas and large bioartificial tissues and facilitate their clinical and research use; 2) produce the device as a mature product by working with a professional manufacturer (Gateway Discovery Products LLC) and cGMP consultant (Cardinal Health); and 3) test the post-thaw in-vitro viability and functionalities of the corneas and bioartificial retinal tissues, and conduct in-vivo animal studies on rabbits (Vision Research Center at UMKC). After achieving these milestones, we will work with our eye bank collaborator (Saving Sight), current distributor (Mayflower Bioscience), and potential strategic partner (Thermo Fisher Scientific) to introduce the product to corneal transplantation and tissue engineering markets.

概括 6.cryocrate.com的青少年有限责任公司的任务是开发新的平台冷冻保存技术用于生命科学和临床实践中的应用。随着细胞基础组织工程的最近快速发展以及再生医学以及移植技术的持续发展迫切需要临床实用方法和有效的设备，以冷冻保存大量库存生物人工组织和供体组织。在该项目的第一阶段时期，我们成功建立了一种新颖的超快速小组织的冷却方法（细胞水平为106k/min）。在第二阶段时期，我们将：1）设计升级的设备系统，可实现整个角膜和大型冷冻保存效率相同的效率生物人工组织并促进其临床和研究用途； 2）通过与专业制造商（Gateway Discovery Products LLC）和CGMP顾问（Cardinal）合作健康）; 3）测试角膜和生物人工视网膜组织的透视后的活力和功能并在兔子（UMKC的视觉研究中心）进行体内动物研究。实现了这些里程碑，我们将与眼库的合作者（储蓄瞄准镜），当前分销商（Mayflower Bioscience）合作，以及潜在的战略合作伙伴（Thermo Fisher Scientific）将产品引入角膜移植和组织工程市场。