An Ultra-fast Cooling Device for Vitrification and Cryopreservation of Cells and Tissues

用于细胞和组织玻璃化和冷冻保存的超快速冷却装置

• 批准号: 10006333
• 负责人: Xu Han
• 金额: $ 75.11万
• 依托单位: CRYOCRATE, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-16 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10006333
• 关键词: Affect; Age related macular degeneration; Animals; Behavioral; Biological; Biological Markers; Biological Sciences; Biomedical Engineering; Biotechnology; Cells; Clinical; Clinical Research; Cornea; Corneal Endothelium; Cryopreservation; Cryopreserved Cell; Cryopreserved Tissue; Crystallization; Cytometry; Data; Development; Device or Instrument Development; Devices; Dimensions; Electrophysiology (science); Endothelial Cells; Endothelium; Engineering; Excision; Eye Banks; Goals; Health; Human; Ice; In Vitro; Keratoplasty; Liquid substance; Manufacturer Name; Measurement; Measures; Methods; Mission; Modeling; Nitrogen; Notification; Oryctolagus cuniculus; Outcome; Patients; Performance; Phase; Process; Production; Regenerative Medicine; Research; Research Institute; Retina; Retinal Diseases; Retinal Pigments; Safety; Sampling; Savings; Sterility; System; Technology; Testing; Thinness; Tissue Donors; Tissue Engineering; Tissue Transplantation; Tissues; Transplantation; Validation; Vision; Vision research; Work; base; cell water; clinical practice; clinically relevant; cryogenics; design; experience; in vivo; meetings; novel; operation; phase 1 study; pre-clinical; preservation; pressure; prototype; regenerative; research and development; retina transplantation; scaffold; simulation; tool; transplant model; user-friendly

Summary The mission of CryoCrate LLC (www.CryoCrate.com) is to develop new platform cryopreservation technologies for applications in life science and clinical practice. With recent rapid advances in cell-based tissue engineering and regenerative medicine, as well as continued development of transplantation technologies, there has always been an urgent need for clinically practical methods and efficient devices for cryopreservation of large stocks of bioartificial and donor tissues. In the Phase I period of this project, we successfully established a novel ultra-fast cooling method (106K/min on cellular level) for small tissues. In the Phase II period, we will: 1) design an upgraded device system that achieves the same efficiency for cryopreservation of the whole corneas and large bioartificial tissues and facilitate their clinical and research use; 2) produce the device as a mature product by working with a professional manufacturer (Gateway Discovery Products LLC) and cGMP consultant (Cardinal Health); and 3) test the post-thaw in-vitro viability and functionalities of the corneas and bioartificial retinal tissues, and conduct in-vivo animal studies on rabbits (Vision Research Center at UMKC). After achieving these milestones, we will work with our eye bank collaborator (Saving Sight), current distributor (Mayflower Bioscience), and potential strategic partner (Thermo Fisher Scientific) to introduce the product to corneal transplantation and tissue engineering markets.

总结