A Novel Cryopreservation Technology for Large Skin Grafts to Facilitate Tissue Banking and Allograft Transplantations

一种用于大型皮肤移植物的新型冷冻保存技术，以促进组织库和同种异体移植

• 批准号: 10011745
• 负责人: Xu Han
• 金额: $ 16.04万
• 依托单位: CRYOCRATE, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-12 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011745
• 关键词: Allografting; Americas; Animal Model; Area; Artificial Intelligence; Autologous Transplantation; Bandage; Big Data; Biological Assay; Clinic; Clinical; Clinical Trials; Collaborations; Communities; Coupled; Cryopreservation; Cryopreserved Tissue; Devices; Engineering; Excision; Family suidae; Freeze Drying; Freezing; Fresh Tissue; Future; Gases; Generations; Goals; Healthcare Industry; Hospitals; Human; Immunogenetics; Impairment; In Situ Nick-End Labeling; In Vitro; Liquid substance; Mechanics; Methods; Missouri; Modeling; Mus; Nitrogen; Organ Procurements; Outcome; Pain; Patients; Performance; Phase; Procedures; Process; Research; Research Project Grants; Sampling; Skin; Skin Tissue; Skin Transplantation; Skin graft; Stains; Surface; System; Technology; Testing; Thick; Thinness; Time; Tissue Donors; Tissue Grafts; Tissue Transplantation; Tissues; Toxic effect; Transplantation; Universities; Variant; base; biobank; clinical application; commercialization; design; experimental study; improved; in vitro Assay; in vivo; invention; medical schools; mouse model; novel; prototype; scale up; skin allograft; success; transplant model; two-dimensional; wound healing

SUMMARY As a result of recent and rapid advances of immunogenetical, artificial intelligence (AI) and big data technologies, the accuracy and efficiency of donor-recipient immunogenetical matching are expected to be significantly improved. As such, traditional tissue banking practices are being severely challenged due to low cryopreservation throughput. Skin allograft transplantations are currently utilized for numerous clinical applications. In such applications, donor tissues are now typically utilized as “expensive bandages”, i.e. for temporary coverage, thereby requiring painful and damaging follow-on autotransplantation procedures. With tremendously improved skin graft cryopreservation methods coupled with use of HLA matching based on AI network, future tissue banking platforms can potentially provide grafts to be used as permanent and definitive treatment for patients, thereby bringing high impact to clinical applications and the healthcare industry. Based on CryoCrate’s novel ultra-fast (106 K/min on a sample surface) cooling technology platform (PCT/US2019/26162), this project aims to develop a highly efficient (approaching 100% post-thaw viability) tissue graft cryopreservation prototype system that requires no cryoprotectant. The post-thaw viability and functionality will be assessed by standard in vitro assays using porcine and human skin (research exempt) grafts and in vivo assays using a mouse model. After achieving these aims, in future phase II and III stages, CryoCrate and its academic collaborator will collaborate with a local Organ Procurement Organization (Mid-America Transplant) and a wound healing clinic (University of Missouri Hospital) for porcine and human skin transplantation studies and clinical trials.

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