An Ultra-fast Cooling Device for Vitrification and Cryopreservation of Cells and Tissues

用于细胞和组织玻璃化和冷冻保存的超快速冷却装置

• 批准号: 8834867
• 负责人: Xu Han
• 金额: $ 15.26万
• 依托单位: CRYOCRATE, LLC
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2017-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8834867
• 关键词: Animal Model; Animals; Biochemical; Biological Testing; Biomedical Research; Blood Vessels; Cells; Cornea; Cryopreservation; Device Designs; Devices; Economics; Engineering; Excision; Family suidae; Film; Future; Genetic Engineering; Genotype; Goals; Heating; Histocompatibility Testing; Human; Infection; Investigation; Life; Liquid substance; Maintenance; Measures; Mechanics; Membrane; Metals; Methods; Nerve Tissue; Nitrogen; Operating System; Optics; Ovarian Tissue; Performance; Phase; Procedures; Production; Psychological reinforcement; Research Activity; Sampling; Skin; Skin Tissue; Solutions; Stainless Steel; Steel; Surface; System; Techniques; Technology; Testing; Thick; Time; Tissue Engineering; Tissue Sample; Tissue Viability; Tissues; Transplantation; Transportation; base; cell type; cryogenics; design; engineering design; evaporation; human tissue; improved; novel; prevent; prototype; public health relevance; seal; stem cell technology; vapor

DESCRIPTION (provided by applicant): Storage and maintenance of valuable genotypes of animal model species as live animal lines would be wholly impractical. However, traditional cryopreservation techniques will be soon found to be inefficient for providing economic approach for cryopreservation of cells and tissues from millions of new animal models being developed. Efficient cryopreservation of clinically valuable tissues, including cornea tissues, ovarian cortical strips, skins, blood vessels, and many others will invaluably benefit corresponding transplantation technology and basic biomedical research using animal model or human tissues. Our overall goal is to develop an ultra-fast cooling device (cooling rate >106 K/min) to achieve vitrification of thin (approximately 100-200 microns) tissues or samples without or with very low concentration of permeating cryoprotectants. We will achieve this by producing the prototype of an ultra-fast cooling device with an application of cutting-edge engineering techniques including cryogenic thin film evaporation and jet impingement cooling. To improve its practical efficiency, the device will be designed as a close operating system that prevents samples from direct contact with liquid nitrogen or its vapor. To achieve those goals, the plan for phase I periods is proposed: 1) design a novel ultra-fast cooling device combining thin film evaporation and jet impingement cooling techniques, determine optimal design parameters for subsystems; 2) produce the device and test its thermal efficiency; and 3) the tested device will be used for porcine and human corneal tissue cryopreservation, and the cryopreserved efficiency will be assessed according to biochemical and histological tests on the post-thaw tissues. At the end of Phase I, the proposed device will be produced under appropriate engineering design control and validated through biological tests.

描述（由申请人提供）：储存和维护有价值的动物模型物种基因型作为活体动物系是完全不切实际的。然而，传统的低温保存技术很快就会被发现效率低下，无法为数百万正在开发的新动物模型的细胞和组织提供经济的低温保存方法。有效的冷冻保存临床有价值的组织，包括角膜组织、卵巢皮质条、皮肤、血管等，将对相应的移植技术和基于动物模型或人体组织的基础生物医学研究具有不可宝贵的价值。我们的总体目标是开发一种超高速冷却装置（冷却速度> 106k /min），以实现薄（约100-200微米）组织或样品的玻璃化，而不需要或只需要极低浓度的渗透冷冻保护剂。我们将通过应用尖端工程技术，包括低温薄膜蒸发和射流撞击冷却，生产超高速冷却装置的原型来实现这一目标。为了提高其实际效率，该装置将被设计成一个封闭的操作系统，以防止样品直接接触液氮或液氮蒸气。为实现这一目标，提出了第一阶段的规划：1)设计一种结合薄膜蒸发和射流冲击冷却技术的新型超快冷却装置，确定子系统的优化设计参数；2)制作装置并测试其热效率；3)将试验装置用于猪和人角膜组织的冷冻保存，并根据解冻后组织的生化和组织学检测来评估冷冻保存效果。在第一阶段结束时，拟议的装置将在适当的工程设计控制下生产，并通过生物试验进行验证。