New tools to translate time across the lifespan in humans and model organisms

翻译人类和模型生物整个生命周期时间的新工具

基本信息

批准号：
10216622
负责人：
Christine J Charvet
金额：
$ 9.34万
依托单位：
DELAWARE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10216622
关键词：
2 year old Adolescence Adult Age Aging Animal Model Animals Behavior Biological Process Birth Brain Callithrix Childhood Communities Comparative Biology Data Data Set Development Developmental Biology Developmental Process Diffusion Discipline Disease Ensure Evolution Fetal Development Functional disorder Gene Expression Generations Genes Genetic Gestational Age Goals Growth Human Longevity Macaca Magnetic Resonance Imaging Medicine Methods Modeling Modification Molecular Motor Cortex Mus Neurons Pan Genus Pathway interactions Pattern Phenotype Primates Production Research Personnel Resources Scanning Scientist Source Structure Study models Time TimeLine Translating Variant Work bioimaging brain pathway cell type course development fetal innovation molecular marker neurogenesis neuroimaging nonhuman primate novel strategies postnatal postnatal development practical application tool tractography transcriptome sequencing web site

项目摘要

Project Summary/Abstract The goal of this project is to identify corresponding ages across postnatal ages in humans and model organisms. This work is needed because scientists often use model organisms such as mice to understand basic biological processes and disorders in humans, but there is currently no resource that enables researchers to find corresponding ages across the lifespan of model organisms and humans. We and others have implemented an easily accessible website to find equivalent developmental ages across 18 mammalian species (http://www.translatingtime.net). This resource is used by a number of researchers who study model organisms (e.g., mice) to translate their findings to humans, but it only finds corresponding ages up to 2 year after birth in humans and its equivalent in model organisms. This is because we focused on sharp changes that occur during neurogenesis (i.e., the production of neurons) as a basis with which to find corresponding ages across species. Neurogenesis occurs largely during fetal development. As a result, we have only identified corresponding ages during fetal and early postnatal development in humans and and model organisms. Yet, many researchers use model organisms to understand human childhood, adolescence, and aging, and there is no resource that enables scientists to find corresponding ages between humans and other species across the lifespan. We will compare the timing of tract (i.e., pathway) maturation and temporal changes in gene expression across species in order to identify corresponding transformations during postnatal and adult ages in humans and their equivalent in model organisms. We will use diffusion MR tractography, which identifies tracts across the brain, to compare the timing of tract maturation across postnatal development in humans, non-human primates, and mice. We will use RNA sequencing to track temporal changes in gene expression across postnatal ages in humans, non-human primates, and mice. Integrating diffusion MR tractography with gene expression is an innovative approach with which to find corresponding ages across the lifespan of humans and model organisms. This project will implement new tools with which to find corresponding ages from model organisms to humans.

项目摘要/摘要该项目的目的是确定人类和模型生物体中产后年龄的相应年龄。需要这项工作，因为科学家经常使用诸如小鼠之类的模型生物来了解基本的生物学人类的过程和疾病，但目前没有资源使研究人员能够找到在模型生物和人类的整个寿命中相应的年龄。我们和其他人已经实施了易于访问的网站，以找到18种哺乳动物的同等发展年龄（http://www.translatingtime.net）。许多研究模型生物的研究人员都使用了此资源（例如，老鼠）将其发现转化为人类，但它只会发现相应的年龄在出生后长达2年人类及其在模型生物中的等效。这是因为我们专注于在神经发生（即神经元的产生）作为在物种之间找到相应年龄的基础。神经发生在很大程度上发生在胎儿发育期间。结果，我们仅确定了相应的年龄在人类和建模生物的胎儿和早期发育期间。但是，许多研究人员使用模型有机体了解人类的童年，青春期和衰老，没有资源使科学家能够在整个生命周期中找到人类与其他物种之间的相应年龄。我们将比较物种基因表达的裂纹（即途径）成熟和时间变化的时机为了确定人类产后和成人年龄期间相应的转变等效于模型生物。我们将使用扩散的MR拖拉术，该拖拉片识别整个大脑的道比较人类，非人类灵长类动物和老鼠。我们将使用RNA测序来跟踪跨产后年龄的基因表达的时间变化人类，非人类灵长类动物和小鼠。与基因表达的扩散MR拖拉术是一种在人类和模型生物的整个寿命中找到相应年龄的创新方法。该项目将实施新工具，以找到从模型生物到人类的相应年龄。