Cell Culture and Cell Phenotyping

资源/服务核心：细胞培养和细胞表型分析模块 摘要 细胞培养和细胞表型分析模块的目标是简化细胞培养和表型分析 视觉研究人员的分析。为了实现这一目标，本模块将支持维护和 对细胞系进行认证，这些细胞系是广泛的NEI重点研究的严格研究所需的 活动，包括所有NEI资助的研究项目和新的合作和试点项目。导演和 该模块的工作人员将提供培训、技术支持和关键设备的维护， 科学严谨，前沿，视觉研究。 本模块的细胞培养部门将提供以下基本服务： 1)永生化细胞系的常规维护。 2)细胞系冻存储备液的维持。 3)原代角膜和视网膜细胞系的建立。 4)支原体和内毒素污染的常规筛查。 5)用于细胞系鉴定的短串联重复序列DNA分析。 该模块的细胞表型分析部门将提供以下综合分析服务： 1)流式细胞仪 2)细胞分类。 3)高通量细胞分析。 4)活细胞代谢分析。 5)微囊泡定量和尺寸测定。 总的来说，这些服务将为校园内所有NEI资助的视觉研究人员提供最先进的 资源，这将提高他们的实验效率，并支持产生高质量，定量 视觉研究各个方面的数据。

Resource/Service Core: Cell Culture and Cell Phenotyping Module Abstract The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. To accomplish this goal, this Module will support the maintenance and authentication of cell lines that are required for rigorous study of a wide range of NEI-focused research activities, including all NEI-funded research projects and new collaborative and pilot projects. The director and staff of this Module will provide training, technical support, and maintenance of key equipment to facilitate scientifically rigorous, cutting edge, vision research. The Cell Culture arm of this module will provide the following essential services: 1) Routine maintenance of immortalized cell lines. 2) Maintenance of cryopreserved stocks of cell lines. 3) Establishment of primary corneal and retinal cell lines. 4) Routine screening for mycoplasma and endotoxin contamination. 5) Short tandem repeat DNA profiling for cell line authentication. The Cell Phenotyping arm of this module will provide the following comprehensive analytical services: 1) Flow cytometry. 2) Cell sorting. 3) High throughput cellular analysis. 4) Live cell metabolic profiling. 5) Microvesicle quantification and size determination. Collectively, these services will provide all NEI-funded vision researchers on campus with state of the art resources that will increase their experimental efficiency and support the generation of high quality, quantitative data for all aspects of vision research.