Cell Culture and Cell Phenotyping

细胞培养和细胞表型分析

• 批准号: 10438809
• 负责人: DANIELLE M. ROBERTSON
• 金额: $ 19.93万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438809
• 关键词: Area; Calendar; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Core Grant; Cornea; Cryopreservation; Data; Development; Endotoxins; Ensure; Equipment; Experimental Designs; Flow Cytometry; Fostering; Funding; Generations; Genetic Fingerprintings; Goals; Grant; Individual; Institution; Laboratories; Maintenance; Metabolic; Methods; Mycoplasma; Ophthalmology; Phenotype; Pilot Projects; Postdoctoral Fellow; Primary Cell Cultures; Records; Reproducibility; Research Activity; Research Personnel; Research Project Grants; Resources; Retina; Schedule; Services; Short Tandem Repeat; Students; Technical Expertise; Training; Vision; Vision research; arm; cell immortalization; design; high throughput screening; instrument; interest; investigator training; microvesicles; operation; programs; routine screening; testing services; vision science

Resource/Service Core: Cell Culture and Cell Phenotyping Module Abstract The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. To accomplish this goal, this Module will support the maintenance and authentication of cell lines that are required for rigorous study of a wide range of NEI-focused research activities, including all NEI-funded research projects and new collaborative and pilot projects. The director and staff of this Module will provide training, technical support, and maintenance of key equipment to facilitate scientifically rigorous, cutting edge, vision research. The Cell Culture arm of this module will provide the following essential services: 1) Routine maintenance of immortalized cell lines. 2) Maintenance of cryopreserved stocks of cell lines. 3) Establishment of primary corneal and retinal cell lines. 4) Routine screening for mycoplasma and endotoxin contamination. 5) Short tandem repeat DNA profiling for cell line authentication. The Cell Phenotyping arm of this module will provide the following comprehensive analytical services: 1) Flow cytometry. 2) Cell sorting. 3) High throughput cellular analysis. 4) Live cell metabolic profiling. 5) Microvesicle quantification and size determination. Collectively, these services will provide all NEI-funded vision researchers on campus with state of the art resources that will increase their experimental efficiency and support the generation of high quality, quantitative data for all aspects of vision research.

资源/服务核心：细胞培养和细胞表型模块 抽象的 细胞培养和细胞表型模块的目的是简化细胞培养和表型 视力研究人员的分析。为了实现这一目标，该模块将支持维护和 严格研究广泛研究的细胞系的身份验证 活动，包括所有由NEI资助的研究项目以及新的合作和试点项目。导演和 该模块的工作人员将提供培训，技术支持和维护关键设备以促进 科学严格的尖端，视觉研究。 该模块的细胞培养部门将提供以下基本服务： 1）常规维持永生的细胞系。 2）维护细胞系的冷冻保存库存。 3）建立原代角膜和视网膜细胞系。 4）定期筛选支原体和内毒素污染。 5）短串联重复DNA分析，用于细胞线认证。 该模块的细胞表型臂将提供以下全面的分析服务： 1）流式细胞仪。 2）细胞分类。 3）高通量细胞分析。 4）活细胞代谢分析。 5）微泡定量和大小确定。 总的来说，这些服务将为校园内的所有NEI资助的视觉研究人员提供最先进的状态 将提高其实验效率并支持高质量，定量的资源 视力研究各个方面的数据。