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Carbon nanotube integrated microdevice for next generation sequencing based virus discovery

用于基于下一代测序的病毒发现的碳纳米管集成微器件

基本信息

批准号：
10226147
负责人：
Siyang Zheng
金额：
$ 52.46万
依托单位：
CARNEGIE-MELLON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-10 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10226147
关键词：
3-Dimensional Bacteria Binding Carbon Nanotubes Cells Cessation of life Clinical Communicable Diseases Complex Coupled Cytolysis Data Devices Diagnosis Disease Disease Outbreaks Economics Elements Engineering Filtration Financial Hardship Genome Goals High-Throughput Nucleotide Sequencing In Situ Influenza Lead Legal patent Methods Microfluidics Mutate Nucleic Acid Binding Nucleic Acids Nucleosomes Patient Care Patients Pattern Performance Pilot Projects Preparation Proteins Publishing Research Research Personnel Resource-limited setting Respiratory Tract Infections Ribosomes Rodent Sampling Science Smooth Pursuit Structure Symptoms Techniques Technology Thick Time Ultrafiltration Viral Viral Genome Virion Virus Virus Diseases Work atomic layer deposition base cost design forest health care economics improved influenzavirus microdevice mortality nanomaterials nanometer new technology next generation sequencing novel pathogen portability simulation social success whole genome zirconium oxide

项目摘要

PROJECT SUMMARY Infectious diseases account for one quarter of global deaths and massive economic loss. Pathogen discovery is critical in combating infectious diseases. However, the causative agents in a large percentage of clinical infectious disease cases remain unidentified. Often occurring in resource-limited areas, major outbreaks were caused by newly emerging viruses, mostly wildlife-originated. The pressing challenges in viral infectious diseases call for a sample-to-answer virus discovery platform that can prepare viral samples from environmental and clinical samples for high throughput sequencing based virus discovery. In this proposed research, we will develop a new carbon nanotube 3D sample preparation and enrichment microdevice (CNT- 3D-SPEM) that employs engineered carbon nanotube material, as shown in our preliminary study, into a new continuous flow microfluidic design for virus particle enrichment. The new microfluidic design has a unique self- regulating flow mechanism to achieve high virus capture efficiency and high sample capacity and flow rate simultaneously. After enrichment, the captured viruses are lyzed inside the CNT-3D-SPEM. The viral genome will be purified and amplified in situ, which are required sample preparation steps for virus discovery through high throughput sequencing. Working with co-investigators together, we will conduct multiple pilot studies that use the developed technology for virus discovery from clinical and environmental samples. The ultimate goal is to develop the entire high-performance portable platform to advance virus discovery for the battle with viral infectious diseases.

项目总结