Metabolism Core

项目总结 代谢组学主要由代谢谱组成，代谢谱是识别和量化的过程。 来自不同生物样本的许多不同生化类别的大量代谢物，包括 细胞、培养液、组织和生物体液。然而，要了解这种疾病的代谢基础 作为癌症，生化机制(S)必须阐明，在这种情况下，有必要介绍适当的 稳定的同位素示踪剂。虽然这极大地增加了要量化的分析物的数量，但它也使 检索更多与生物相关的信息。稳定同位素分解代谢组学(SIRM) 方法是新陈代谢核心的一个独特的专业，由具有40多年综合专业知识的PIs领导 SIRM。新陈代谢核心的目标是为广泛覆盖的代谢组提供服务 同分异构体和同源异构体分析，以及高通量蛋白质分析。为了实现这一点，我们利用 四大分析平台，即(1)高分辨率核磁共振(核磁共振)，(2)超高 分辨率傅里叶变换质谱仪(UHR-FTMS)，有或没有层析，(3)超痕量 用电感耦合等离子体质谱(ICPMS)进行元素分析，以及(4)反相蛋白质阵列(RPPA)。 新陈代谢核心人员将就分析方法和基于SIRM的实验设计提供建议 开发其他技术，以解决鉴定和量化以及生物学方面的具体问题 与科布雷项目相关的解释。因为实验设计和样品制备是不可分割的 对于数据获取和解释，新陈代谢核心提供这些和信息学方面的专业知识 与SIRM方法相关。这些目标将按照以下具体目标实现。特定的 目标1.提供广泛的核磁共振、MS和RPPA技术的访问和专业知识，以 调查人员。具体目标2.提供实验设计、样品处理和支持 数据整理、代谢途径和生化机制分析的研究人员。特定目标 3.开发和实施新的方法，以增强与Cobre相关的代谢能力 项目。该核心将通过提供先进的分析技术和 在癌症发展和行为的代谢需求方面的专业知识。

PROJECT SUMMARY “Metabolomics” largely consists of metabolic profiling, which is the process of identification and quantification of a large number of metabolites of many different biochemical classes, from diverse biological samples, including cells, culture media, tissue and biofluids. However, to understand the metabolic underpinning of diseases such as cancer, biochemical mechanism(s) must be elucidated, in which case it is necessary to introduce appropriate stable isotope tracers. Although this greatly increases the number of analytes to be quantified, it also enables the retrieval of much more biologically relevant information. This Stable Isotope Resolved Metabolomics (SIRM) approach is a unique specialty of the Metabolism Core, led by PIs with over 40 years of combined expertise in SIRM. The goals of the Metabolism Core are to provide services for a wide coverage of the metabolome with isotopomer and isotopologue analysis, and high throughput protein analysis. To achieve this, we make use of four major analytical platforms, namely (1) high resolution nuclear magnetic resonance (NMR), (2) ultra-high resolution Fourier-transform mass spectrometry (UHR-FTMS), with or without chromatography, (3) ultra-trace elemental analyses by inductively-coupled plasma MS (ICPMS), and (4) Reverse Phase Protein Arrays (RPPA). The Metabolism Core personnel will advise on analytical methods and SIRM-based experimental design with development of additional techniques to address specific issues of identification and quantification and biological interpretation relevant to the COBRE Projects. Because experimental design and sample preparation are integral to the data acquisition and interpretation, the Metabolism Core provides expertise in these and informatics relevant to the SIRM approach. These goals will be achieved according to the following Specific Aims. Specific Aim 1. To provide access and expertise across a wide range of NMR, MS and RPPA technologies to investigators. Specific Aim 2. To provide experimental design, sample processing, and support to the investigators for data reduction, metabolic pathway, and biochemical mechanism analyses. Specific Aim 3. To develop and implement new methods to enhance metabolic capabilities relevant to the COBRE Projects. This Core will support the young investigators by providing advanced analytical technologies and expertise in the metabolic requirements for cancer development and behavior.