Metabolism Core

新陈代谢核心

• 批准号: 10573145
• 负责人: ANDREW N. LANE
• 金额: $ 20.32万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10573145
• 关键词: Address; Algorithms; Behavior; Biochemical; Biochemistry; Bioinformatics Shared Resource; Biological; Biology; Biomass; Biometry; Cancer Center; Carbon; Cells; Centers of Research Excellence; Chromatography; Classification; Collaborations; Consult; Culture Media; Data; Data Analyses; Databases; Development; Dinucleoside Phosphates; Disease; Electrophoresis; Experimental Designs; Fourier Transform; Genomics; Goals; Human Resources; Inductively Coupled Plasma Mass Spectrometry; Informatics; Infusion procedures; Isotope Labeling; Isotopes; Kentucky; Liquid Chromatography; Literature; Logistic Regressions; Malignant Neoplasms; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Metabolism; Methods; Mus; Nuclear Magnetic Resonance; Operative Surgical Procedures; Organism; Output; Oxidation-Reduction; Pathway Analysis; Phase; Preparation; Principal Investigator; Procedures; Process; Protein Analysis; Protein Array; Proteins; Research Personnel; Resolution; Retrieval; Sampling; Schedule; Services; Source; Standardization; Statistical Data Interpretation; System; Techniques; Technology; Time; Tissue Harvesting; Tissues; Trace Elements; Tracer; Universities; Update; Validation; advanced analytics; analytical method; cell growth; data acquisition; data reduction; high throughput analysis; medical specialties; metabolic abnormality assessment; metabolome; metabolomics; stable isotope; tumor metabolism; ultra high resolution; web site

PROJECT SUMMARY “Metabolomics” largely consists of metabolic profiling, which is the process of identification and quantification of a large number of metabolites of many different biochemical classes, from diverse biological samples, including cells, culture media, tissue and biofluids. However, to understand the metabolic underpinning of diseases such as cancer, biochemical mechanism(s) must be elucidated, in which case it is necessary to introduce appropriate stable isotope tracers. Although this greatly increases the number of analytes to be quantified, it also enables the retrieval of much more biologically relevant information. This Stable Isotope Resolved Metabolomics (SIRM) approach is a unique specialty of the Metabolism Core, led by PIs with over 40 years of combined expertise in SIRM. The goals of the Metabolism Core are to provide services for a wide coverage of the metabolome with isotopomer and isotopologue analysis, and high throughput protein analysis. To achieve this, we make use of four major analytical platforms, namely (1) high resolution nuclear magnetic resonance (NMR), (2) ultra-high resolution Fourier-transform mass spectrometry (UHR-FTMS), with or without chromatography, (3) ultra-trace elemental analyses by inductively-coupled plasma MS (ICPMS), and (4) Reverse Phase Protein Arrays (RPPA). The Metabolism Core personnel will advise on analytical methods and SIRM-based experimental design with development of additional techniques to address specific issues of identification and quantification and biological interpretation relevant to the COBRE Projects. Because experimental design and sample preparation are integral to the data acquisition and interpretation, the Metabolism Core provides expertise in these and informatics relevant to the SIRM approach. These goals will be achieved according to the following Specific Aims. Specific Aim 1. To provide access and expertise across a wide range of NMR, MS and RPPA technologies to investigators. Specific Aim 2. To provide experimental design, sample processing, and support to the investigators for data reduction, metabolic pathway, and biochemical mechanism analyses. Specific Aim 3. To develop and implement new methods to enhance metabolic capabilities relevant to the COBRE Projects. This Core will support the young investigators by providing advanced analytical technologies and expertise in the metabolic requirements for cancer development and behavior.

项目摘要 “代谢组学”主要由代谢谱组成，代谢谱是鉴定和定量代谢产物的过程。 来自不同生物样品的许多不同生化类别的大量代谢物，包括 细胞、培养基、组织和生物流体。然而，为了了解疾病的代谢基础， 作为癌症，必须阐明生物化学机制，在这种情况下，有必要引入适当的 稳定同位素示踪剂尽管这大大增加了待定量的分析物的数量，但它也使得能够 获取更多的生物学相关信息。稳定同位素解析代谢组学（Stable Isotope Resolved Metabolomics，SIRM） 方法是代谢核心的独特专业，由PI领导，拥有超过40年的综合专业知识， 长官代谢核心的目标是为代谢组的广泛覆盖提供服务， 同位素异构体和同位素物分析以及高通量蛋白质分析。为了实现这一目标，我们利用 四大分析平台，即（1）高分辨率核磁共振（NMR），（2）超高 分辨率傅里叶变换质谱法（UHR-FTMS），有或没有色谱法，（3）超痕量 通过电感耦合等离子体MS（ICPMS）进行元素分析，和（4）反相蛋白质阵列（RPPA）。 代谢核心人员将就分析方法和基于SIRM的实验设计提供建议， 开发更多技术，以解决识别和量化以及生物 与COBRE项目相关的解释。因为实验设计和样品制备是一体的 对于数据采集和解释，代谢核心提供这些和信息学方面的专业知识 与SIRM方法有关。这些目标将根据以下具体目标实现。具体 目标1.提供广泛的NMR、MS和RPPA技术的访问权限和专业知识， investigators.具体目标2。提供实验设计、样品处理和支持， 数据处理、代谢途径和生化机制分析的研究人员。具体目标 3.开发和实施新方法，以增强与COBRE相关的代谢能力 项目该核心将通过提供先进的分析技术， 癌症发展和行为的代谢要求方面的专业知识。