Metabolism Core

新陈代谢核心

• 批准号: 10573145
• 负责人: ANDREW N. LANE
• 金额: $ 20.32万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-03-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10573145
• 关键词: Address; Algorithms; Behavior; Biochemical; Biochemistry; Bioinformatics Shared Resource; Biological; Biology; Biomass; Biometry; Cancer Center; Carbon; Cells; Centers of Research Excellence; Chromatography; Classification; Collaborations; Consult; Culture Media; Data; Data Analyses; Databases; Development; Dinucleoside Phosphates; Disease; Electrophoresis; Experimental Designs; Fourier Transform; Genomics; Goals; Human Resources; Inductively Coupled Plasma Mass Spectrometry; Informatics; Infusion procedures; Isotope Labeling; Isotopes; Kentucky; Liquid Chromatography; Literature; Logistic Regressions; Malignant Neoplasms; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Metabolism; Methods; Mus; Nuclear Magnetic Resonance; Operative Surgical Procedures; Organism; Output; Oxidation-Reduction; Pathway Analysis; Phase; Preparation; Principal Investigator; Procedures; Process; Protein Analysis; Protein Array; Proteins; Research Personnel; Resolution; Retrieval; Sampling; Schedule; Services; Source; Standardization; Statistical Data Interpretation; System; Techniques; Technology; Time; Tissue Harvesting; Tissues; Trace Elements; Tracer; Universities; Update; Validation; advanced analytics; analytical method; cell growth; data acquisition; data reduction; high throughput analysis; medical specialties; metabolic abnormality assessment; metabolome; metabolomics; stable isotope; tumor metabolism; ultra high resolution; web site

PROJECT SUMMARY “Metabolomics” largely consists of metabolic profiling, which is the process of identification and quantification of a large number of metabolites of many different biochemical classes, from diverse biological samples, including cells, culture media, tissue and biofluids. However, to understand the metabolic underpinning of diseases such as cancer, biochemical mechanism(s) must be elucidated, in which case it is necessary to introduce appropriate stable isotope tracers. Although this greatly increases the number of analytes to be quantified, it also enables the retrieval of much more biologically relevant information. This Stable Isotope Resolved Metabolomics (SIRM) approach is a unique specialty of the Metabolism Core, led by PIs with over 40 years of combined expertise in SIRM. The goals of the Metabolism Core are to provide services for a wide coverage of the metabolome with isotopomer and isotopologue analysis, and high throughput protein analysis. To achieve this, we make use of four major analytical platforms, namely (1) high resolution nuclear magnetic resonance (NMR), (2) ultra-high resolution Fourier-transform mass spectrometry (UHR-FTMS), with or without chromatography, (3) ultra-trace elemental analyses by inductively-coupled plasma MS (ICPMS), and (4) Reverse Phase Protein Arrays (RPPA). The Metabolism Core personnel will advise on analytical methods and SIRM-based experimental design with development of additional techniques to address specific issues of identification and quantification and biological interpretation relevant to the COBRE Projects. Because experimental design and sample preparation are integral to the data acquisition and interpretation, the Metabolism Core provides expertise in these and informatics relevant to the SIRM approach. These goals will be achieved according to the following Specific Aims. Specific Aim 1. To provide access and expertise across a wide range of NMR, MS and RPPA technologies to investigators. Specific Aim 2. To provide experimental design, sample processing, and support to the investigators for data reduction, metabolic pathway, and biochemical mechanism analyses. Specific Aim 3. To develop and implement new methods to enhance metabolic capabilities relevant to the COBRE Projects. This Core will support the young investigators by providing advanced analytical technologies and expertise in the metabolic requirements for cancer development and behavior.

项目摘要 “代谢组学”在很大程度上包括代谢分析，这是识别和数量的过程 来自各种生物学样本的许多不同生化类别的代谢产物，包括 细胞，培养基，组织和生物流体。但是，要了解疾病的代谢基础 作为癌症，必须阐明生化机制，在这种情况下，有必要引入适当的 稳定的同位素示踪剂。尽管这大大增加了要量化的分析物的数量，但也可以启用 检索更多与生物学相关的信息。这种稳定的同位素解析代谢组学（SIRM） 方法是新陈代谢核心的独特专业，由PI领导，拥有超过40年的联合专业知识 Sirm。新陈代谢核心的目标是为与代谢的广泛报道提供服务 同位素和同位素学分析以及高通量蛋白分析。为了实现这一目标，我们利用 四个主要的分析平台，即（1）高分辨率核磁共振（NMR），（2）超高 分辨率傅立叶转换质谱法（UHR-FTM），有或没有色谱，（3）超轨道 通过电感耦合等离子体MS（ICPM）和（4）反相蛋白阵列（RPPA）进行元素分析。 代谢核心人员将向分析方法和基于SIRM的实验设计提供建议 开发其他技术来解决特定的识别和量化问题和生物学问题 解释与毛茸茸的项目有关。因为实验设计和样品制剂是不可或缺的 对于数据获取和解释，新陈代谢核心在这些信息和信息方面提供了专业知识 与Sirm方法有关。这些目标将根据以下特定目标实现。具体的 目标1。在广泛的NMR，MS和RPPA技术中提供访问和专业知识 调查人员。具体目的2。为实验设计，样本处理和支持 降低数据，代谢途径和生化机制分析的具体目的。 3。开发和实施新方法以增强与毛病相关的代谢能力 项目。该核心将通过提供先进的分析技术和 癌症发展和行为的代谢需求方面的专业知识。