Protein Production and Biochemical Characterization Core

蛋白质生产和生化表征核心

• 批准号: 10219095
• 负责人: ANGELA M. GRONENBORN
• 金额: $ 30.2万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-27 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219095
• 关键词: Biochemical; Biological Assay; Biophysics; Chemicals; Collaborations; Complex; Core Protein; Cryoelectron Microscopy; Funding; Goals; HIV; HIV-1; Human; Methodology; Mission; Production; Proteins; Recombinant Proteins; Recombinants; Research Personnel; Roentgen Rays; Services; Structure; Validation; Viral Proteins; Virus; Work; member; milligram; novel; protein complex; protein expression; protein purification; screening; success; tool; virology

Protein Core Abstract High quality proteins are essential for the success of structural studies. The Protein Core will support any PCHPI- initiated projects and collaborators by producing milligram quantities of homogeneous recombinant viral protein, human cellular proteins and their complexes for structural studies. The Core will also carry out biophysical and bioanalytical characterization of proteins and protein complexes to support and validate structural studies. To that end the Core will work in an iterative fashion with the NMR Core to generate proteins suitable for structural studies and will provide protein and complexes to the X-ray Core and Cryo-EM Core for screening and structure determination. The Core will also communicate on a regular basis with the Interactions Discovery Core and HIV Virology Core, which together will provide information to the Protein Core on the discovery of novel target proteins and/or their validation, thus getting new complexes ready to enter the structure determination pipeline. Finally, the Core will support all Projects by making proteins for project goals.

蛋白质核心摘要 高质量的蛋白质对于结构研究的成功至关重要。蛋白质核心将支持任何 PCHPI- 通过生产毫克量的同质重组病毒启动项目和合作者 蛋白质、人类细胞蛋白质及其复合物，用于结构研究。核心区还将开展 蛋白质和蛋白质复合物的生物物理和生物分析表征以支持和验证 结构研究。为此，Core 将以迭代方式与 NMR Core 一起工作以生成蛋白质 适用于结构研究，并将为 X 射线核心和 Cryo-EM 核心提供蛋白质和复合物 筛选和结构测定。核心还将定期与交互部门进行沟通 发现核心和 HIV 病毒学核心，它们将共同向蛋白质核心提供有关 发现新的靶蛋白和/或其验证，从而使新的复合物准备好进入结构 测定管道。最后，核心将通过为项目目标制造蛋白质来支持所有项目。