Tick Resources Core

勾选资源核心

• 批准号: 10222516
• 负责人: Ulrike Gertrud Munderloh
• 金额: $ 19.25万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-13 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10222516
• 关键词: Adult; American Type Culture Collection; Anaplasma phagocytophilum; Animal Model; Arthropods; Basic Science; Biochemical; Biological Assay; Black-legged Tick; Blood; Borrelia burgdorferi; Cell Culture Techniques; Cell Line; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Collection; Communities; Complement; Contracts; Deposition; Development; Entomology; Exposure to; Genes; Genetic; Genome; Goals; Immune; Immune response; Immune signaling; Immune system; Immunity; Immunologics; Immunology; In Vitro; Infection; Investigation; Knock-out; Knowledge; Laboratories; Mass Spectrum Analysis; Membrane; Microbiology; Minnesota; Modification; Molecular Analysis; Mutagenesis; National Institute of Allergy and Infectious Disease; Nymph; Oral; Pathogenesis; Pathway interactions; Peptides; Plasmids; Proteomics; RNA Interference; RNA delivery; Reagent; Reporter; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rickettsia; Source; System; Techniques; Testing; Ticks; Training; Transcript; Translational Research; Universities; Work; base; biodefense; cost; differential expression; efficacy testing; functional genomics; gene product; genome editing; germ free condition; immune function; in vivo; insight; member; microbial; microbiota; microorganism interaction; new technology; novel; nuclease; overexpression; pathogen; professor; repository; response; symbiont; tool; transcriptomics; vector tick; web site

TICK RESOURCES CORE Abstract: The overarching goal of the Tick Resources Core within this P01 application is to provide support to all three research projects and share reagents, materials and new technologies with the scientific community. Requests from the community will be handled through the Biodefense and Emerging Infections Research Resources Repository (BEI) website (www.beiresources.org). BEI was established by the National Institute of Allergy and Infectious Diseases and has been managed under contract by the American Type Culture Collection for fifteen years. Dr. Ulrike Munderloh, a Professor at the University of Minnesota, will be the director of this technical core and is ideally suited to manage the facility. First, she has been a pioneer in the field of tick-pathogen interactions by developing and refining techniques for mutagenesis, plasmid-based complementation, and functional genomics for rickettsial agents in addition to inventing a membrane feeder system for ticks. Second, her laboratory has contributed to 22 out of 30 Rickettsiales species for sequencing in the “Rickettsiales Genomes” project, and supplied 10 of the 14 isolates deposited in BEI. Third, Dr. Munderloh has an outstanding record of distributing tick cell lines and microbial isolates to the scientific community. Aim#1 of the Tick Resources Core will provide currently available tick-vector-based tools to support the research for all three research projects within this P01 application. Aim#2 of the Tick Resources Core will develop novel cell lines suitable for analyses of immune pathways that are activated or repressed in response to pathogens, symbionts and selected microbiota. This will be achieved through RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), reporter tags and overexpression assays. Model organisms have a myriad of tools that facilitate the study of pathogen-arthropod interactions. They also provide critical insights to the field of entomology, microbiology and immunology. However, we present strong evidence in this P01 application that Ixodes scapularis ticks seem to be built on entirely different principles that govern microbial pathogenesis and immunity. Therefore, a strong Tick Resources Core is of utmost importance to support all three Projects within this P01 application. By providing existing I. scapularis-based tools and developing novel technologies to investigators, the Tick Resources Core will contribute to uncover novel paradigms in I. scapularis-microbial interactions. This scientific knowledge has potential implications for basic and translational science.

勾选资源核心 摘要：此 P01 应用程序中 Tick 资源核心的总体目标是为 所有三个研究项目并与科学界分享试剂、材料和新技术。 社区的请求将通过生物防御和新发感染研究来处理 资源库 (BEI) 网站 (www.beiresources.org)。 BEI是由国家研究院成立的 过敏和传染病，并由美国典型文化公司签约管理 收藏十五年。明尼苏达大学教授 Ulrike Munderloh 博士将担任主任 该技术核心的核心，非常适合管理该设施。首先，她是该领域的先驱 通过开发和完善基于质粒的诱变技术来实现蜱与病原体的相互作用 除了发明膜饲养器之外，还研究了立克次体病原体的互补和功能基因组学 蜱虫系统。其次，她的实验室对 30 种立克次氏体中的 22 种进行了测序 “立克次体基因组”项目，并提供了 BEI 保藏的 14 种分离株中的 10 种。三、蒙德罗博士 在向科学界分发蜱细胞系和微生物分离株方面拥有出色的记录。目标#1 蜱资源核心的将提供当前可用的基于蜱向量的工具来支持以下研究： 此 P01 应用程序中的所有三个研究项目。 Tick 资源核心的目标#2 将开发新型细胞 适合分析响应病原体而激活或抑制的免疫途径的细胞系， 共生体和选定的微生物群。这将通过 RNA 干扰、定期聚类来实现 间隔短回文重复序列 (CRISPR/Cas9)、报告标签和过表达测定。模型 生物体有无数的工具可以促进病原体与节肢动物相互作用的研究。他们还提供 对昆虫学、微生物学和免疫学领域的重要见解。然而，我们提供了强有力的证据 在这个 P01 应用中，肩胛硬蜱似乎建立在完全不同的控制原理之上 微生物发病机制和免疫。因此，强大的Tick资源核心对于 支持此 P01 应用程序中的所有三个项目。通过提供现有的基于肩胛肌的工具和 Tick 资源核心为研究人员开发新技术，将有助于发现新颖的技术 肩胛肌-微生物相互作用的范例。这些科学知识对基础知识具有潜在影响 和转化科学。