Tick Resources Core

勾选资源核心

• 批准号: 9976331
• 负责人: Ulrike Gertrud Munderloh
• 金额: $ 19.25万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-13 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9976331
• 关键词: Adult; American Type Culture Collection; Anaplasma phagocytophilum; Animal Model; Arthropods; Basic Science; Biochemical; Biological Assay; Black-legged Tick; Blood; Borrelia burgdorferi; Cell Culture Techniques; Cell Line; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Collection; Communities; Complement; Contracts; Deposition; Development; Entomology; Exposure to; Genes; Genetic; Genome; Goals; Immune; Immune response; Immune signaling; Immune system; Immunity; Immunologics; Immunology; In Vitro; Infection; Investigation; Knock-out; Knowledge; Laboratories; Mass Spectrum Analysis; Membrane; Microbiology; Minnesota; Modification; Molecular Analysis; Mutagenesis; National Institute of Allergy and Infectious Disease; Nymph; Oral; Pathogenesis; Pathway interactions; Peptides; Plasmids; Proteomics; RNA Interference; RNA delivery; Reagent; Reporter; Research; Research Personnel; Research Project Grants; Research Support; Resources; Rickettsia; Source; System; Techniques; Testing; Ticks; Training; Transcript; Translational Research; Universities; Work; base; biodefense; cost; differential expression; efficacy testing; functional genomics; gene product; genome editing; germ free condition; immune function; in vivo; insight; member; microbial; microbiota; microorganism interaction; new technology; novel; nuclease; overexpression; pathogen; professor; repository; response; symbiont; tool; transcriptomics; vector tick; web site

TICK RESOURCES CORE Abstract: The overarching goal of the Tick Resources Core within this P01 application is to provide support to all three research projects and share reagents, materials and new technologies with the scientific community. Requests from the community will be handled through the Biodefense and Emerging Infections Research Resources Repository (BEI) website (www.beiresources.org). BEI was established by the National Institute of Allergy and Infectious Diseases and has been managed under contract by the American Type Culture Collection for fifteen years. Dr. Ulrike Munderloh, a Professor at the University of Minnesota, will be the director of this technical core and is ideally suited to manage the facility. First, she has been a pioneer in the field of tick-pathogen interactions by developing and refining techniques for mutagenesis, plasmid-based complementation, and functional genomics for rickettsial agents in addition to inventing a membrane feeder system for ticks. Second, her laboratory has contributed to 22 out of 30 Rickettsiales species for sequencing in the “Rickettsiales Genomes” project, and supplied 10 of the 14 isolates deposited in BEI. Third, Dr. Munderloh has an outstanding record of distributing tick cell lines and microbial isolates to the scientific community. Aim#1 of the Tick Resources Core will provide currently available tick-vector-based tools to support the research for all three research projects within this P01 application. Aim#2 of the Tick Resources Core will develop novel cell lines suitable for analyses of immune pathways that are activated or repressed in response to pathogens, symbionts and selected microbiota. This will be achieved through RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), reporter tags and overexpression assays. Model organisms have a myriad of tools that facilitate the study of pathogen-arthropod interactions. They also provide critical insights to the field of entomology, microbiology and immunology. However, we present strong evidence in this P01 application that Ixodes scapularis ticks seem to be built on entirely different principles that govern microbial pathogenesis and immunity. Therefore, a strong Tick Resources Core is of utmost importance to support all three Projects within this P01 application. By providing existing I. scapularis-based tools and developing novel technologies to investigators, the Tick Resources Core will contribute to uncover novel paradigms in I. scapularis-microbial interactions. This scientific knowledge has potential implications for basic and translational science.

TICK资源核心 翻译后摘要：在这个P01应用程序中的蜱资源核心的首要目标是提供支持， 所有三个研究项目都将与科学界分享试剂、材料和新技术。 来自社区的请求将通过生物防御和新出现的感染研究来处理 资源库（BEI）网站（www.beiresources.org）。BEI是由美国国家科学院 过敏和传染病，并已根据合同由美国典型文化管理 收藏了十五年。明尼苏达大学教授Ulrike Munderloh博士将担任主任 这一技术核心，非常适合管理设施。首先，她一直是该领域的先驱， 通过开发和改进基于质粒的诱变技术， 互补作用和立克次体因子的功能基因组学， 系统为tick。第二，她的实验室已经为30种立克次体中的22种做出了贡献， “立克次体基因组”项目，并提供了14个菌株中的10个存放在BEI。第三，蒙德洛博士 在向科学界分发蜱细胞系和微生物分离物方面有着出色的记录。目标1 的蜱虫资源核心将提供目前可用的蜱虫矢量为基础的工具，以支持研究， P01申请中的所有三个研究项目。Tick Resources Core的目标2将开发新的细胞 适用于分析响应于病原体而被激活或抑制的免疫途径的线， 共生体和选定的微生物群。这将通过RNA干扰来实现， 间隔短回文重复序列（CRISPR/Cas9）、报告标签和过表达测定。模型 生物体有无数的工具，便于研究病原体-节肢动物的相互作用。他们还提供 对昆虫学、微生物学和免疫学领域的重要见解。然而，我们提出了强有力的证据， 在这个P01应用中，肩突硬蜱似乎是建立在完全不同的原则上的， 微生物致病和免疫。因此，一个强大的蜱资源核心是至关重要的， 支持此P01应用程序中的所有三个项目。通过提供现有的I。基于肩胛骨的工具， 为研究人员开发新技术，蜱虫资源核心将有助于发现新的 在I.肩胛骨-微生物相互作用。这一科学知识对基本的 和转化科学。