Tick Resources Core

Tick资源核心 摘要：此P01应用程序中的Tick Resources Core的总体目标是为 所有三个研究项目，并与科学界共享试剂、材料和新技术。 来自社区的请求将通过生物防御和新发感染研究来处理 资源库(BEI)网站(www.beiresource ces.org)。BEI是由国家科学技术研究所建立的 过敏和传染病，并已由美国典型文化合同管理 收藏了十五年。明尼苏达大学教授Ulrike Munderloh博士将担任主任 是这一技术核心的核心，非常适合管理该设施。首先，她是这一领域的先驱 以质粒为基础的诱变技术的开发和改进 除了发明膜饲养器外，立克次体制剂的互补和功能基因组学 扁虱的系统。其次，她的实验室已经为30种立克次体中的22种进行了测序 “立克次体基因组”项目，并提供了保存在BEI的14个分离物中的10个。第三，曼德罗博士 在向科学界分发扁虱细胞系和微生物分离物方面有着出色的记录。目标1 将提供当前可用的基于滴答向量的工具，以支持对 这三个研究项目都在P01申请范围内。Tick Resources Core的Aim#2将开发新细胞 适合分析对病原体反应被激活或被抑制的免疫途径的品系， 共生体和选定的微生物区系。这将通过定期聚集的RNA干扰来实现 间隔短回文重复序列(CRISPR/Cas9)、报告标签和过度表达分析。型号 生物体有无数的工具来促进病原体-节肢动物相互作用的研究。它们还提供 对昆虫学、微生物学和免疫学领域的重要见解。然而，我们提出了强有力的证据 在这个P01应用程序中，肩部硬蜱似乎是建立在完全不同的支配原则上的 微生物致病机制与免疫。因此，一个强大的Tick Resources Core对于 支持此P01应用程序中的所有三个项目。通过提供现有的基于肩胛骨的工具和 为调查人员开发新技术，Tick Resources Core将为发现小说做出贡献 肩胛肌-微生物相互作用的范例。这一科学知识对基础知识具有潜在的影响 和翻译科学。

TICK RESOURCES CORE Abstract: The overarching goal of the Tick Resources Core within this P01 application is to provide support to all three research projects and share reagents, materials and new technologies with the scientific community. Requests from the community will be handled through the Biodefense and Emerging Infections Research Resources Repository (BEI) website (www.beiresources.org). BEI was established by the National Institute of Allergy and Infectious Diseases and has been managed under contract by the American Type Culture Collection for fifteen years. Dr. Ulrike Munderloh, a Professor at the University of Minnesota, will be the director of this technical core and is ideally suited to manage the facility. First, she has been a pioneer in the field of tick-pathogen interactions by developing and refining techniques for mutagenesis, plasmid-based complementation, and functional genomics for rickettsial agents in addition to inventing a membrane feeder system for ticks. Second, her laboratory has contributed to 22 out of 30 Rickettsiales species for sequencing in the “Rickettsiales Genomes” project, and supplied 10 of the 14 isolates deposited in BEI. Third, Dr. Munderloh has an outstanding record of distributing tick cell lines and microbial isolates to the scientific community. Aim#1 of the Tick Resources Core will provide currently available tick-vector-based tools to support the research for all three research projects within this P01 application. Aim#2 of the Tick Resources Core will develop novel cell lines suitable for analyses of immune pathways that are activated or repressed in response to pathogens, symbionts and selected microbiota. This will be achieved through RNA interference, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), reporter tags and overexpression assays. Model organisms have a myriad of tools that facilitate the study of pathogen-arthropod interactions. They also provide critical insights to the field of entomology, microbiology and immunology. However, we present strong evidence in this P01 application that Ixodes scapularis ticks seem to be built on entirely different principles that govern microbial pathogenesis and immunity. Therefore, a strong Tick Resources Core is of utmost importance to support all three Projects within this P01 application. By providing existing I. scapularis-based tools and developing novel technologies to investigators, the Tick Resources Core will contribute to uncover novel paradigms in I. scapularis-microbial interactions. This scientific knowledge has potential implications for basic and translational science.