Applied Research Component: Improving genetic resources available to the research community

应用研究部分：改善研究界可用的遗传资源

• 批准号: 10225745
• 负责人: KEVIN R COOK
• 金额: $ 8.43万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10225745
• 关键词: Aging; Animal Model; Applied Research; Behavior; Biological; Biological Models; Biological Process; Cell Proliferation; Cells; Cellular biology; Characteristics; Collaborations; Collection; Communities; Complex; Consultations; Country; Databases; Development; Discipline; Disease; Double-Stranded RNA; Drosophila genus; Drosophila melanogaster; Epithelial; Equilibrium; Eukaryota; Evolution; Expenditure; Fees; Funding; Future; Genes; Genetic; Genome; Genotype; Goals; Grant; Health; Human; Income; Indiana; Individual; Information Management; Information Resources; Insecta; Institutes; International; Intestines; Investigation; Investments; Maintenance; Metabolism; Methodology; Modeling; Molecular; Neurobiology; Organism; Pattern; Physiology; Population Biology; RNA Interference; Recovery; Research; Research Personnel; Research Project Grants; Research Support; Resource Development; Resources; Role; Sampling; Scientist; Services; Ships; Source; Technology; Tissues; Transgenes; Transgenic Organisms; United States National Institutes of Health; Universities; Work; base; cost; disease phenotype; drug discovery; efficacy evaluation; fly; genetic resource; genetic strain; genetic technology; human disease; human model; improved; knock-down; medical specialties; operation; preservation; programs; self-renewal; success; tool; trend; web site

Project Summary The Bloomington Drosophila Stock Center (BDSC) supports a large, worldwide community of scientists using the insect Drosophila melanogaster as a model organism for biomedical experimentation. The goals of the BDSC are to provide a collection of documented living stocks of broad value to current research, to preserve documented strains with clear future value, and to provide information and support services that promote maximal exploitation of these materials. These goals facilitate research by providing universal and rapid access to the most generally useful stocks, by preserving specialty genotypes with exceptional characteristics, and by providing information that helps researchers identify stocks appropriate to their needs. Drosophila is used extensively in studies of biological processes relevant to human health and investigations of molecular mechanisms underlying disease, because genetic technologies available to Drosophila researchers are among the most sophisticated in any multicellular organism. As the most comprehensive source of stocks for genetic experimentation with Drosophila, the BDSC is central to the success of many research projects including a large number of NIH grants. The first specific aim of this proposal is to continue acquiring, maintaining and distributing Drosophila strains and to continue developing associated information resources to meet the research needs of Drosophila scientists while maintaining and promoting excellent user support. Key to this aim is the administration and advancement of the highly successful cost recovery program that finances operational expenses from user fees. Consequently, the proposal focuses on support and development of the core management team as the most effective way to leverage the investment of NIH resources. The second specific aim, which will comprise 10% of grant expenditures, is to undertake research to increase the utility of the two largest subsets of BDSC stocks. Stocks carrying GAL4 or split-GAL4 transgenes are used to force expression of other transgenes in cell-specific patterns, allowing investigators to manipulate cell characteristics experimentally in otherwise normal individuals. They form the bases of many experimental approaches in Drosophila and most Drosophila models of human disease mechanisms, and the research here aims to characterize their expression patterns in the intestine, the most useful model system for studying the cell proliferation dynamics of a self-renewing epithelial tissue. Stocks carrying transgenes capable of expressing double-stranded RNAs under the control of GAL4 or split-GAL4 transgenes are used to knock down expression of specific genes via RNA interference mechanisms. A large collection of these stocks has been generated and preliminarily characterized. The research proposed here, in collaboration with the Transgenic RNAi Project at Harvard University, will evaluate the efficacy of these stocks in greater depth.

项目摘要 布卢明顿果蝇库存中心（BDSC）支持一个大型的，世界范围内的科学家社区， 昆虫果蝇作为生物医学实验的模式生物。的目标 BDSC将提供一系列对当前研究具有广泛价值的记录在案的活种群，以保护 具有明确未来价值的记录菌株，并提供信息和支持服务， 最大限度地利用这些资源。这些目标通过提供普遍和快速的 通过保存具有特殊特征的特殊基因型， 并提供信息，帮助研究人员确定适合他们需要的种群。果蝇是 广泛用于与人类健康相关的生物过程研究和分子生物学研究。 疾病的潜在机制，因为果蝇研究人员可用的遗传技术是 是多细胞生物中最复杂的作为遗传学最全面的股票来源， BDSC是许多研究项目成功的关键，包括 大量的NIH赠款。这项建议的第一个具体目标是继续获得、维持和 分发果蝇品系，并继续开发相关的信息资源， 果蝇科学家的研究需求，同时保持和促进优秀的用户支持。的关键 目标是管理和推进非常成功的成本回收计划， 运营费用来自用户费。因此，该提案的重点是支持和发展 核心管理团队是利用NIH资源投资的最有效途径。第二 具体目标将占补助金支出的10%，是进行研究以提高 BDSC股票的两个最大子集。携带GAL4或分裂GAL4转基因的原种用于迫使 以细胞特异性模式表达其他转基因，使研究人员能够操纵细胞特性 在其他方面正常的人身上进行实验。它们构成了许多实验方法的基础， 果蝇和大多数人类疾病机制的果蝇模型，这里的研究旨在 表征它们在肠中的表达模式，肠是研究细胞的最有用的模型系统。 自我更新上皮组织的增殖动力学。携带能够表达 在GAL4或分裂GAL4转基因控制下的双链RNA用于敲低 通过RNA干扰机制表达特定基因。这些股票的大量收藏已经被 生成并初步表征。这项研究与转基因生物研究所合作， 哈佛大学的RNAi项目将更深入地评估这些股票的功效。