Bloomington Drosophila Stock Center at Indiana University

印第安纳大学布卢明顿果蝇库存中心

• 批准号: 10396349
• 负责人: KEVIN R COOK
• 金额: $ 35.22万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10396349
• 关键词: Address; Affect; Animal Model; Area; Back; Biological Process; Biomedical Research; COVID-19 pandemic; Cell physiology; Cells; Collection; Communities; Computer software; Contracts; Development; Disasters; Drosophila genus; Drosophila melanogaster; Employee; Enteroendocrine Cell; Funding; Genetic; Grant; Guidelines; Human; Human Resources; Indiana; Information Centers; Information Management; Intervention; Intestines; Laboratories; Management Information Systems; Midgut; Modeling; Online Systems; Productivity; Quality Control; Research; Research Personnel; Research Project Grants; Resource Development; Resources; Sampling; Scientist; Services; Time; Transgenes; United States National Institutes of Health; Universities; Work; cell type; computer program; coronavirus disease; experience; fly; human disease; intestinal epithelium; migration; operation; pandemic disease; parent grant; peptide hormone; prevent; repaired; repository; success; trustworthiness

Project Summary The Bloomington Drosophila Stock Center (BDSC) collects, curates, maintains and distributes strains of the fly Drosophila melanogaster to support the biomedical research community. It is the largest and most comprehensive Drosophila stock collection in the world, and it is central to the success of many research projects—including over 820 active NIH grants. The COVID pandemic has caused several aspects of BDSC operations to suffer, and the funding requested here will serve to repair damage and restore normal productivity in the areas of information system management, quality control and research by engaging the temporary services of available professionals within Indiana University. First, contracted computer programming services will restore appropriate progress to an ongoing project transitioning BDSC information management to a web-based platform. Unfortunately, this project was slowed irreparably by diverting effort of BDSC scientists to disaster management. These services will help push the project to completion before legacy software critical to all BDSC functions is no longer usable. Second, short-term employees will allow the BDSC to redeploy the effort of experienced stockkeeping staff to catch up on the surveillance of stocks for cross-contamination or genetic breakdown that was suspended when COVID restrictions mandated only essential work on campus. Identifying problematic stocks is critical to assuring that the samples investigators receive are trustworthy. Finally, an experienced technician will devote effort to a resource-development project generating strains needed for investigating intestinal epithelial cell functions. The parent grant supports the identification and characterization of GAL4 and split-GAL4 drivers that allow targeted expression of UAS transgenes in specific midgut cells, but the pandemic prevented the expected rate of progress. Specifically, it delayed the introduction of new, more-effective drivers to scientists investigating the development and functions of enteroendocrine cells, a model peptide hormone-secreting cell type with human cognates. Altogether, these interventions will repair COVID-inflicted operational deficiencies and allow the BDSC to provide services to biomedical researchers at the high level of quality that existed before the pandemic.

项目摘要 布卢明顿果蝇储备中心（BDSC）收集、管理、维护和分发果蝇品系 果蝇，以支持生物医学研究界。它是世界上最大、 这是世界上最全面的果蝇种群收集，也是许多研究成功的关键。 项目-包括820多个活跃的NIH赠款。COVID大流行导致BDSC的几个方面 行动受到影响，这里要求的资金将用于修复损害和恢复正常 提高资讯系统管理、品质控制及研究工作的效率。 印第安纳州大学内可用的专业人员的临时服务。第一，承包电脑 编程服务将恢复正在进行的项目的适当进度，转换BDSC信息 管理到一个基于Web的平台。不幸的是，这个项目被无法挽回地拖慢了， BDSC的科学家进行灾难管理。这些服务将有助于推动项目完成， 对所有BDSC功能至关重要的遗留软件不再可用。第二，短期雇员将允许 BDSC将重新部署经验丰富的库存管理人员的工作，以赶上库存监控， 交叉污染或基因分解，当COVID限制仅强制执行时， 校园里的重要工作确定有问题的库存对于确保样本调查人员 接受是值得信赖的。最后，一位经验丰富的技术人员将致力于资源开发项目 产生研究肠上皮细胞功能所需的菌株。父母补助金支持 允许UAS靶向表达GAL 4和分裂GAL 4驱动子的鉴定和表征 在特定的中肠细胞中的转基因，但大流行阻止了预期的进展速度。具体 推迟了向研究发展的科学家介绍新的、更有效的驱动因素， 肠内分泌细胞的功能，与人类同源的模型肽分泌细胞类型。 总之，这些干预措施将修复COVID造成的运营缺陷，并使BDSC能够 向生物医学研究人员提供与大流行病发生前一样高质量的服务。