Yale Center for Genome Analysis Shared Resource

耶鲁大学基因组分析中心共享资源

• 批准号: 10228168
• 负责人: SHRIKANT M MANE
• 金额: $ 28.45万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10228168
• 关键词: Applications Grants; Authorship; Bioinformatics; Biological Assay; Biological Sciences; Biotechnology; Blood specimen; Cancer Biology; Cancer Center Support Grant; Cells; Complement; Computer software; Consultations; Custom; DNA; DNA sequencing; Data; Data Analyses; Doctor of Philosophy; Educational workshop; Epigenetic Process; Equipment; Experimental Designs; Expression Profiling; Funding; Gene Expression; Genetic; Genetic Transcription; Genomics; Genotype; Grant; High Performance Computing; Institutes; Ions; Laboratories; Large-Scale Sequencing; Libraries; Linux; Malignant Neoplasms; Methodology; Methods; Methylation; Mission; Preparation; Protocols documentation; Publications; RNA; Research; Research Personnel; Resource Sharing; Resources; Sampling; Scientist; Sequence Analysis; Services; Structure; Technology; Testing; Time; Tissues; Training; Training and Education; United States National Institutes of Health; Variant; Vendor; Wages; Work; Writing; Yale Cancer Center; cost; data quality; design; experience; experimental study; genome analysis; genomic platform; improved; instrument; interest; member; new technology; next generation; next generation sequencing; professor; research and development; secondary analysis; sequencing platform; square foot; tool; transcriptome sequencing

YALE CENTER FOR GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Yale Center for Genome Analysis (YCGA) was established in 2010 to provide next-generation sequencing (NGS), single-cell genomics, and microarray support forYCC members. The Center is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and next-Gen DNA sequencing and related state-of-the-art technologies. Full microarray and next-generation sequencing services provided by YCGA include: DNA and RNA isolation from tissue and blood; sample preparation ready for microarray and next-Gen sequence (NGS) analysis; providing primary data; full-time YCC-supported staff for downstream bioinformatics data analysis, working collaboratively with YCC investigators and providing methods and other write-ups for grants. Additionally, the staff has obtained equipment and center grants to reduce the cost of sequencing. During the most recent funding period, 211 people have used YCGA Eighty-seven of these users (41%) were YCC members. The majority of this use came from Genomics, Genetics and Epigenetics (GGE). YCGA provides free consultation and training for YCC members. The core is led by Director Shrikant Mane, PhD, Professor of Genetics, whose experience is complemented by the strong expertise of Associate Director Kaya Bilguvar, MD, PhD. The Specific Aims of YCGA are to provide: (1) Next-generation sequencing services using multiple sequencing platforms, including Illumina, Ion Torrent, and Pacific Biosciences; (2) Microarray services using Affymetrix and Illumina platforms for gene expression, genotyping, and methylation assays; (3) Single cell genomics and structural variation determination using the 10X Genomics platform; (4) Research and development. YCGA continuously carries out biotechnology research to develop improved methodologies and tools, to test the relative merits of new technologies, and to generate preliminary results for NIH grants. (5) Bioinformatics. Dr. Francesc Lopez supports the secondary analysis needs of YCC members. His salary is supported by YCC. (6) Consultation. YCGA makes every effort to develop collaborative relationships with its users, and takes interest in their scientific projects. (7) Education and training. Staff members actively participate in YCC Grand Rounds, departmental seminars, and YSM Dean’s workshops aimed at providing principles and technical details of the various genomic technologies.

耶鲁基因组分析中心共享资源 项目总结/摘要 耶鲁大学基因组分析中心（YCGA）成立于2010年，旨在提供下一代测序 (NGS)单细胞基因组学和微阵列支持YCC成员。该中心是一个全方位的服务设施 致力于提供RNA表达谱分析、DNA基因分型和下一代DNA测序及相关的 最先进的技术。YCGA提供的完整微阵列和下一代测序服务 包括：从组织和血液中分离DNA和RNA;准备用于微阵列和下一代 序列（NGS）分析;提供原始数据;全职YCC支持的下游生物信息学工作人员 数据分析，与YCC调查人员合作，并提供方法和其他书面报告， 赠款。此外，工作人员还获得了设备和中心赠款，以降低测序成本。 在最近的资助期间，有211人使用了YCGA，其中87人（41%）是 YCC成员。其中大部分来自基因组学、遗传学和表观遗传学（GGE）。 YCGA为YCC会员提供免费咨询和培训。核心是由导演什里坎特马内， 博士，遗传学教授，其经验与副主任的强大专业知识相辅相成 卡亚比尔古瓦，医学博士，哲学博士。 YCGA的具体目标是提供：（1）使用多重测序的下一代测序服务 平台，包括Illumina、Ion Torrent和Pacific Biosciences;（2）使用Affyphase和 用于基因表达、基因分型和甲基化测定的Illumina平台;（3）单细胞基因组学和 使用10 X Genomics平台确定结构变异;（4）研究和开发。YCGA 不断开展生物技术研究，以开发更好的方法和工具，测试 新技术的相对优点，并产生初步的结果，美国国立卫生研究院赠款。(5)生物信息学。博士 Francesc洛佩斯支持YCC成员的二次分析需求。他的工资由YCC提供。（六） 协商YCGA尽一切努力发展与用户的合作关系，并采取兴趣 他们的科学项目。(7)教育培训工作人员积极参加青年商会的圆桌会议， 部门研讨会，和YSM院长的讲习班，旨在提供的原则和技术细节， 各种基因组技术。