Yale Microarray Center for Research on the Nervous System

描述（由申请人提供）： 为了让联盟批准的尽可能多的 NINDS 和 NIMH 支持的研究人员能够使用最先进的微阵列技术，我们建议在耶鲁大学建立一个微阵列中心，该中心将以强大的耶鲁神经科学项目和凯克基金会生物技术资源实验室为基础建立坚实的基础和基础设施。来自 Keck Affymetrix 基因芯片、DNA 微阵列和生物统计资源的工作人员将提供一支极其专业和高度敬业的团队，确保该中心的快速建立和最终成功。该中心将 1) 向尽可能多的 NINDS 和 NIMH 支持的研究人员提供全面、最先进的 Affymetrix、定制和通用斑点载玻片以及 ABI 1700 DNA 微阵列分析，2) 提供 DNA 微阵列和生物统计培训和教育，3) 提供专业服务（例如定制神经科学阵列、激光捕获显微镜和小样本扩增方法）并开展技术研究需要进一步加强DNA微阵列分析的应用，以解决具有挑战性的神经科学研究问题； 4）提供生物统计服务，开发用于神经组织DNA微阵列分析的改进分析工具，并将计算密集型分析转移到耶鲁生物医学超级计算机上； 5) 开发一个 NINDS/NIMH Web 界面，用于将 DNA 微阵列数据提交到微阵列联盟关系数据仓库中。该应用程序的优势包括凯克实验室丰富的专业知识、基础设施和仪器，将为该中心提供支持； Keck 实验室每年向超过 350 个机构的超过 1,100 名主要研究人员提供最先进的微阵列、Affymetrix、生物统计和其他技术（按服务收费）的经验； Keck 微阵列资源在监督最近由 U24 支持的 NIDDK 微阵列生物技术中心方面的经验；新的耶鲁大学生物医学高性能计算中心将为拟议的微阵列中心的用户提供独特的机会，以解决适合并行计算解决方案的计算挑战；以及耶鲁大学医学院对该中心的大力支持，其中包括为该中心配备 ABI 1700 扫描仪所需的费用分摊。

DESCRIPTION (provided by applicant): To bring the most advanced microarray technologies within reach of the largest possible number of NINDS and NIMH-supported investigators approved by the Consortium, we propose to establish a Microarray Center at Yale that will build on a substantial foundation and infrastructure that begins with the very strong Yale neuroscience program and with the Keck Foundation Biotechnology Resource Laboratory. Staff from the Keck Affymetrix GeneChip, DNA Microarray, and Biostatistics Resources will provide an extremely expert and highly dedicated team that will ensure the rapid establishment of the Center and its ultimate success. The Center will 1) provide comprehensive, state-of-the-art Affymetrix, custom and generic spotted glass slide, and ABI 1700 DNA microarray analyses to as many NINDS and NIMH-supported investigators as can be supported by the requested funding and available instrumentation, 2) provide DNA microarray and biostatistical training and education, 3) provide specialized services (e.g., custom neuroscience arrays, laser capture microscopy, and small sample amplification methods) and carry out the technology research needed to further enhance the application of DNA microarray analysis to solve challenging neuroscience research problems; 4) provide biostatistical services, develop improved analytical tools for DNA microarray analysis of neurological tissues, and move computing intensive analyses onto the Yale Biomedical supercomputer; 5) develop a NINDS/NIMH Web interface for submission of DNA microarray data into the Microarray Consortium relational data warehouse. Strengths of this application include the extensive expertise, infrastructure, and instrumentation in the Keck Lab that will support the Center; the experience of the Keck Lab at providing state-of-the-art microarray, Affymetrix, biostatistical and other technologies on a service charge basis to >1,100 principal investigators annually from >350 institutions; the experience of the Keck Microarray Resource at overseeing the recent U24-supported, NIDDK Microarray Biotechnology Center; the new Yale Center for High Performance Computation in Biomedicine that will provide unique opportunities for users of the proposed Microarray Center to solve computational challenges that are amenable to a parallel computing solution; and the very strong support of the Yale School of Medicine for the Center and which includes the cost sharing needed to equip the Center with an ABI 1700 scanner.