Yale Center for Genome Analysis Shared Resource

耶鲁大学基因组分析中心共享资源

• 批准号: 10461895
• 负责人: SHRIKANT M MANE
• 金额: $ 28.45万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10461895
• 关键词: Applications Grants; Authorship; Bioinformatics; Biological Assay; Biological Sciences; Biotechnology; Blood specimen; Cancer Biology; Cancer Center Support Grant; Cells; Complement; Computer software; Consultations; Custom; DNA; DNA sequencing; Data; Data Analyses; Doctor of Philosophy; Educational workshop; Epigenetic Process; Equipment; Experimental Designs; Expression Profiling; Funding; Gene Expression; Genetic; Genetic Transcription; Genomics; Genotype; Grant; High Performance Computing; Institutes; Ions; Laboratories; Large-Scale Sequencing; Libraries; Linux; Malignant Neoplasms; Methodology; Methods; Methylation; Mission; Persons; Preparation; Protocols documentation; Publications; RNA; Research; Research Personnel; Resource Sharing; Resources; Sampling; Scientist; Sequence Analysis; Services; Technology; Testing; Time; Tissues; Training; Training and Education; United States National Institutes of Health; Variant; Vendor; Wages; Work; Writing; Yale Cancer Center; cost; data quality; design; experience; experimental study; genome analysis; genomic platform; improved; instrument; interest; member; new technology; next generation; next generation sequencing; professor; research and development; secondary analysis; sequencing platform; square foot; tool; transcriptome sequencing

YALE CENTER FOR GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Yale Center for Genome Analysis (YCGA) was established in 2010 to provide next-generation sequencing (NGS), single-cell genomics, and microarray support forYCC members. The Center is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and next-Gen DNA sequencing and related state-of-the-art technologies. Full microarray and next-generation sequencing services provided by YCGA include: DNA and RNA isolation from tissue and blood; sample preparation ready for microarray and next-Gen sequence (NGS) analysis; providing primary data; full-time YCC-supported staff for downstream bioinformatics data analysis, working collaboratively with YCC investigators and providing methods and other write-ups for grants. Additionally, the staff has obtained equipment and center grants to reduce the cost of sequencing. During the most recent funding period, 211 people have used YCGA Eighty-seven of these users (41%) were YCC members. The majority of this use came from Genomics, Genetics and Epigenetics (GGE). YCGA provides free consultation and training for YCC members. The core is led by Director Shrikant Mane, PhD, Professor of Genetics, whose experience is complemented by the strong expertise of Associate Director Kaya Bilguvar, MD, PhD. The Specific Aims of YCGA are to provide: (1) Next-generation sequencing services using multiple sequencing platforms, including Illumina, Ion Torrent, and Pacific Biosciences; (2) Microarray services using Affymetrix and Illumina platforms for gene expression, genotyping, and methylation assays; (3) Single cell genomics and structural variation determination using the 10X Genomics platform; (4) Research and development. YCGA continuously carries out biotechnology research to develop improved methodologies and tools, to test the relative merits of new technologies, and to generate preliminary results for NIH grants. (5) Bioinformatics. Dr. Francesc Lopez supports the secondary analysis needs of YCC members. His salary is supported by YCC. (6) Consultation. YCGA makes every effort to develop collaborative relationships with its users, and takes interest in their scientific projects. (7) Education and training. Staff members actively participate in YCC Grand Rounds, departmental seminars, and YSM Dean’s workshops aimed at providing principles and technical details of the various genomic technologies.

耶鲁大学基因组分析共享资源中心 项目概要/摘要 耶鲁大学基因组分析中心 (YCGA) 成立于 2010 年，提供下一代测序服务 (NGS)、单细胞基因组学和为 YCC 成员提供的微阵列支持。该中心是一个提供全方位服务的设施 致力于提供 RNA 表达谱、DNA 基因分型和下一代 DNA 测序及相关服务 最先进的技术。 YCGA提供完整的微阵列和新一代测序服务 包括：从组织和血液中分离 DNA 和 RNA；为微阵列和下一代做好准备的样品制备 序列（NGS）分析；提供原始数据； YCC 支持的下游生物信息学全职员工 数据分析，与 YCC 研究人员合作并提供方法和其他文章 补助金。此外，工作人员还获得了设备和中心拨款，以降低测序成本。 在最近的资助期间，有 211 人使用过 YCGA，其中 87 人 (41%) 是 YCC会员。大部分用途来自基因组学、遗传学和表观遗传学 (GGE)。 YCGA为YCC会员提供免费咨询和培训。核心由导演Shrikant Mane领衔， 博士，遗传学教授，副主任的强大专业知识补充了他的经验 卡亚·比尔古瓦尔（Kaya Bilguvar），医学博士、哲学博士。 YCGA的具体目标是提供：（1）使用多重测序的下一代测序服务 平台，包括 Illumina、Ion Torrent 和 Pacific Biosciences； (2) 使用 Affymetrix 和的微阵列服务 用于基因表达、基因分型和甲基化检测的 Illumina 平台； (3) 单细胞基因组学和 使用 10X Genomics 平台确定结构变异； (4)研究开发。 YCGA 不断开展生物技术研究，开发改进的方法和工具，测试 新技术的相对优点，并为 NIH 拨款产生初步结果。 (5)生物信息学。博士。 Francesc Lopez 支持 YCC 会员的二次分析需求。他的工资由YCC 承担。 (6) 咨询。 YCGA 尽一切努力与用户发展合作关系，并关注 在他们的科学项目中。 （七）教育培训。员工积极参与YCC大回合， 部门研讨会和 YSM 院长研讨会旨在提供原则和技术细节 各种基因组技术。