Yale Center for Genome Analysis Shared Resource

耶鲁大学基因组分析中心共享资源 项目摘要/摘要 耶鲁大学基因组分析中心(YCGA)成立于2010年，旨在提供下一代测序 (NGS)、单细胞基因组学和为YCC成员提供微阵列支持。该中心是一个提供全方位服务的设施 致力于提供RNA表达分析、DNA基因分型和下一代DNA测序及相关 最先进的技术。YCGA提供完整的微阵列和下一代测序服务 包括：从组织和血液中分离DNA和RNA；为微阵列和下一代做好样品准备 序列(NGS)分析；提供原始数据；YCC支持的下游生物信息学全职工作人员 数据分析，与YCC调查人员协作，并提供方法和其他撰写 资助金。此外，工作人员还获得了设备和中心赠款，以降低排序成本。 在最近的资助期内，有211人使用过YCGA，其中87人(41%)是 YCC会员。其中大部分使用来自基因组学、遗传学和表观遗传学(GGE)。 YCGA为YCC会员提供免费咨询和培训。核心由施里坎特·马内主任领导， 博士，遗传学教授，副主任的强大专业知识补充了他的经验 Kaya Bilguvar，医学博士，博士 YCGA的具体目标是提供：(1)使用多个测序的下一代测序服务 平台，包括Illumina、Ion Torrent和Pacific Biosciences；(2)使用Affymetrix和 用于基因表达、基因分型和甲基化分析的Illumina平台；(3)单细胞基因组学和 利用10X基因组学平台进行结构变异测定；(4)研究与开发。YCGA 持续进行生物技术研究，以开发改进的方法和工具，以测试 新技术的相对优点，并为国家卫生研究院的拨款产生初步结果。(5)生物信息学。Dr。 Francesc Lopez支持YCC成员的二级分析需求。他的工资是由YCC资助的。(6) 咨询。YCGA尽一切努力发展与用户的协作关系，并对此感兴趣 在他们的科学项目中。(七)教育培训。工作人员积极参与YCC大赛， 部门研讨会和油麻地系主任工作坊，旨在提供 各种基因组技术。

YALE CENTER FOR GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Yale Center for Genome Analysis (YCGA) was established in 2010 to provide next-generation sequencing (NGS), single-cell genomics, and microarray support forYCC members. The Center is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and next-Gen DNA sequencing and related state-of-the-art technologies. Full microarray and next-generation sequencing services provided by YCGA include: DNA and RNA isolation from tissue and blood; sample preparation ready for microarray and next-Gen sequence (NGS) analysis; providing primary data; full-time YCC-supported staff for downstream bioinformatics data analysis, working collaboratively with YCC investigators and providing methods and other write-ups for grants. Additionally, the staff has obtained equipment and center grants to reduce the cost of sequencing. During the most recent funding period, 211 people have used YCGA Eighty-seven of these users (41%) were YCC members. The majority of this use came from Genomics, Genetics and Epigenetics (GGE). YCGA provides free consultation and training for YCC members. The core is led by Director Shrikant Mane, PhD, Professor of Genetics, whose experience is complemented by the strong expertise of Associate Director Kaya Bilguvar, MD, PhD. The Specific Aims of YCGA are to provide: (1) Next-generation sequencing services using multiple sequencing platforms, including Illumina, Ion Torrent, and Pacific Biosciences; (2) Microarray services using Affymetrix and Illumina platforms for gene expression, genotyping, and methylation assays; (3) Single cell genomics and structural variation determination using the 10X Genomics platform; (4) Research and development. YCGA continuously carries out biotechnology research to develop improved methodologies and tools, to test the relative merits of new technologies, and to generate preliminary results for NIH grants. (5) Bioinformatics. Dr. Francesc Lopez supports the secondary analysis needs of YCC members. His salary is supported by YCC. (6) Consultation. YCGA makes every effort to develop collaborative relationships with its users, and takes interest in their scientific projects. (7) Education and training. Staff members actively participate in YCC Grand Rounds, departmental seminars, and YSM Dean’s workshops aimed at providing principles and technical details of the various genomic technologies.