CHARACTERIZING AND IMPROVING HUMANIZED IMMUNE SYSTEM MOUSE MODELS (IMM-HIS)

人源化免疫系统小鼠模型 (IMM-HIS) 的表征和改进

• 批准号: 10282228
• 负责人: MEGAN SYKES
• 金额: $ 219.87万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2023-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10282228
• 关键词: Alleles; Autoantigens; CD34 gene; Cells; Communities; Family suidae; Generations; HLA-DQ8 antigen; Hematopoietic stem cells; Human; Immune system; Intravenous; Mus; Reproducibility; Skin graft; T cell reconstitution; Thymic Tissue; Thymus Gland; Tissues; Training; Transgenic Organisms; Transplantation; Umbilical Cord Blood; Validation; fetal; human fetal thymus; immune function; immune reconstitution; improved; islet; member; mouse model; neonatal human; reconstitution

The study focuses on the ability to substitute MHC-defined porcine fetal thymus tissue for human fetal thymus tissue for the construction of human immune system (HIS) mice. The study explores the ability to substitute MHC-defined porcine fetal thymus tissue for human fetal thymus tissue for the construction of humanized immune system (HIS) mice. T cell reconstitution and function in HIS mice constructed with fetal pig thymic tissue will be compared to mice HIS mice constructed from cord blood CD34+ cells, followed by an attempt to improve reconstitution and function by porcine thymus by using pigs that are transgenic for common HLA alleles.

本研究旨在探讨以猪胎胸腺组织替代人胎胸腺组织构建人免疫系统（HIS）小鼠的能力。本研究探讨了用猪胎胸腺组织替代人胎胸腺组织构建人源化免疫系统（HIS）小鼠的能力。将用胎猪胸腺组织构建的HIS小鼠中的T细胞重建和功能与用脐带血CD34+细胞构建的小鼠HIS小鼠进行比较，然后尝试通过使用对常见HLA等位基因转基因的猪来改善猪胸腺的重建和功能。