Impact of DNA double-strand breaks on 3D genome organization and genome stability in Alzheimer’s disease
DNA 双链断裂对阿尔茨海默病 3D 基因组组织和基因组稳定性的影响
基本信息
- 批准号:10282373
- 负责人:
- 金额:$ 13.67万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-15 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalATAC-seqAdoptedAdvisory CommitteesAgingAlzheimer&aposs DiseaseAlzheimer&aposs disease brainAlzheimer&aposs disease modelAlzheimer&aposs disease riskAutopsyBiological AssayBiologyBrainCellsChIP-seqChromatinChromosomal translocationCoculture TechniquesComplexDNADNA BindingDNA DamageDNA Double Strand BreakDataDevelopmentDiseaseFrequenciesGene ExpressionGenomeGenome StabilityGenomic InstabilityGenomicsGoalsHi-CHumanHyperactivityImmediate-Early GenesInduced pluripotent stem cell derived neuronsKnockout MiceLabelLeadLocationMeasuresMediatingMentorsMentorshipMicrogliaMissionModelingMolecularMosaicismMusMutationNerve DegenerationNeurodegenerative DisordersNeurogliaNeuronal DysfunctionNeuronsNonhomologous DNA End JoiningOligodendrogliaOrthologous GenePartner in relationshipPathogenesisPathologicPathway interactionsPatternPharmacologyPhasePlayPublic HealthPublishingRecurrenceReportingRoleStructureTauopathiesTechnologyTestingTherapeutic InterventionTrainingWorkaging braincell typechromosome conformation capturecohesincomputational pipelinesexperiencefamilial Alzheimer diseaseimprovedin situ sequencinginduced pluripotent stem cellinduced pluripotent stem cell technologymouse modelnervous system disordernovelnovel therapeuticspresenilin-1preventpromoterresponsesingle cell analysissingle-cell RNA sequencingtherapeutic evaluationtooltranscription factortranscriptome
项目摘要
PROJECT SUMMARY/ABSTRACT
The accumulation of DNA Double-Strand Breaks (DSBs) in neurons is an early hallmark of Alzheimer’s disease
(AD). Increased DSBs are also associated with aging, which is the largest risk factor for AD. AD is also a complex
disease involving all major brain glial cell types. Thus, there is a critical need to understand the molecular
mechanisms of DSB induced changes in both neurons and glia. The structural stability of the genome is
paramount in maintaining a functional genome, and recently, the 3D organization of the genome has emerged
as a major regulator of genome function. My overall hypothesis is that 3D genome reorganization and structural
genome instability mediated by DSBs are principle drivers of AD pathogenesis and brain aging. My objective is
to determine how and to what extent DSBs within the neurons impact genome organization and the glial
response, with the goal of identifying molecular pathways that can be targeted as novel therapies for preventing
or halting the progression of neurodegeneration. I will use mouse models that recapitulate the pre-symptomatic
accumulation of DSBs in neurons and human iPSC models to determine the degree of the 3D genome disruption
caused due to DSBs and the underlying molecular mechanisms. I will also identify the consequences of neuronal
DSBs on the structural stability of the genome by measuring the frequency of DSB mediated chromosomal
translocations in human AD neurons. Interestingly, normal neuronal activity causes DSBs at immediate early
gene promoters. Also, neuronal hyperactivity has been reported in AD. I will test if IEGs are locations of frequent
chromosomal translocations after neuronal hyperactivity induced in neuronal culture.
Previous studies have shown that microglia transitions to a reactive state in response to
neurodegeneration. To understand the role of the 3D genome organization in this microglia transition, I will use
single cell Hi-C to measure the unique chromatin interactions that mediate the reactive microglia state.
Subsequently, transcription factor predictions using integrative analysis of single cell Hi-C will be tested in iPSC
derived microglia-neuron co-cultures for their potential to modulate the reactive microglia response. This
approach will be extended to study oligodendrocyte response in the independent phase.
I will work with my mentor Dr. Li-Huei Tsai, my mentorship committee, Dr. Bruce Yankner and Dr. Manolis
Kellis, my technical support and advisory committee, Dr. Frederick Alt and Dr. Peter Fraser to carry out my
proposed training plan. I will gain experience in iPSC technology and differentiation from the Tsai lab to modulate
the disease associated microglia response to neuronal DSBs and work with Dr. Manolis Kellis to implement the
computational pipelines for the transcription factor predictions. To bridge the gap in my training in the biology of
AD and brain aging, I will audit relevant courses and receive additional mentoring from Dr. Bruce Yankner.
项目总结/摘要
神经元中DNA双链断裂(DSB)的积累是阿尔茨海默病的早期标志
(AD)。增加的DSB也与衰老有关,这是AD的最大风险因素。AD也是一种复杂的
涉及所有主要脑胶质细胞类型的疾病。因此,迫切需要了解分子
DSB引起神经元和胶质细胞变化的机制。基因组的结构稳定性是
在维持功能基因组方面至关重要,最近,基因组的3D组织已经出现
作为基因组功能的主要调节器。我的总体假设是,3D基因组重组和结构
由DSB介导的基因组不稳定性是AD发病机制和脑老化的主要驱动因素。我的目标是
为了确定神经元内的DSB如何以及在多大程度上影响基因组组织和神经胶质细胞,
反应,目标是确定可以作为预防新疗法的分子途径,
或者阻止神经退化的进程我将使用小鼠模型,
DSB在神经元和人iPSC模型中的积累,以确定3D基因组破坏的程度
由DSB和潜在的分子机制引起。我还将确定神经元
通过测量DSB介导的染色体频率来研究DSB对基因组结构稳定性的影响
在人类AD神经元中的易位。有趣的是,正常的神经元活动导致DSB在即刻早期,
基因启动子此外,在AD中已经报道了神经元活动过度。我将测试IEGs是否是频繁
在神经元培养中诱导神经元过度活跃后的染色体易位。
先前的研究表明,小胶质细胞在对细胞凋亡的反应中转变为反应性状态。
神经变性为了理解3D基因组组织在小胶质细胞转变中的作用,我将使用
单细胞Hi-C来测量介导反应性小胶质细胞状态的独特染色质相互作用。
随后,将在iPSC中测试使用单细胞Hi-C的整合分析的转录因子预测
衍生的小胶质细胞-神经元共培养物具有调节反应性小胶质细胞反应的潜力。这
该方法将扩展到研究独立期少突胶质细胞的反应。
我将与我的导师蔡丽惠博士、我的导师委员会、布鲁斯扬克纳博士和马诺利斯博士一起工作
Kellis,我的技术支持和咨询委员会,Frederick Alt博士和Peter Fraser博士执行我的
提出培训计划。我将从Tsai实验室获得iPSC技术和分化的经验,
疾病相关的小胶质细胞对神经元DSB的反应,并与Manolis Kellis博士合作,
用于转录因子预测的计算管道。为了弥补我在生物学训练中的差距,
AD和大脑老化,我将旁听相关课程,并接受布鲁斯扬克纳博士的额外指导。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Vishnu Dileep', 18)}}的其他基金
Impact of DNA double-strand breaks on 3D genome organization and genome stability in Alzheimer’s disease
DNA 双链断裂对阿尔茨海默病 3D 基因组组织和基因组稳定性的影响
- 批准号:
10463836 - 财政年份:2021
- 资助金额:
$ 13.67万 - 项目类别:
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