Center for Live Cell Genomics

摘要 活细胞基因组学中心 我们将为大规模、长期、廉价、模块化、 可定制、共享、物联网控制、可重复的活细胞培养和基于组织的 实验性基因组疾病模型组织模型包括传统的细胞培养以及 从手术或活组织检查获得的类器官和原代组织外植体。有机体工厂配套 组织的生长和维持将与外部和芯片上的电光流控分析相结合， 模块成为以开源软件为模型的生态系统的一部分。它将使用 商用传感器、摄像头和计算机，连接在使用简单3D技术灵活设计的平台上 印刷、模制、蚀刻和研磨技术可在任何机构获得。这将 促进组织培养实验平台的快速创新。 我们将推动这项技术，并利用其一流的能力， 神经发育和儿科癌症，解决了一些大问题。哪些基因贡献最大 对人类大脑发育有重要影响吗他们怎么会在 神经发育疾病还是脑损伤哪些特定的分子通路被破坏， 儿童癌症的个案我们如何在组织模型中测试特定途径的治疗方法 具体到每一位患者？ 我们的教育和推广计划包括一个培训计划，以培养一个多元化和包容性的群体 通过中学和社区接受基因组科学培训的本科生 大学外展以及编码研讨会和研究为基础的实验室课程，为UCSC 培养核心竞争力。参加这些活动是 对研究生和博士后进行包容性教学法和导师制培训。我们也在开发 一站式信息中心，形成一个在线社区，并通过 沉浸式网络研讨会和教程，面向更广泛的受众， 公众对研究机构的科学家和临床医生。 我们的工作将使神经科学和癌症研究和教育取得重大进展， 促进细胞生物学和基因组学新的开源文化，使科学和 超越精英机构的教育访问，将NHGRI ANvIL等共享项目扩展到数据之外 和代码，包括实验和互联网连接的实验平台。

ABSTRACT Center for Live Cell Genomics We will build new methodology and capacity for large-scale, long-term, inexpensive, modular, customizable, shared, Internet-of-Things-controlled, reproducible live cell culture and tissue-based experimental genomics disease models. Tissue models include traditional cell culture as well as organoid and primary tissue explants obtained from surgery or biopsy. Organoid factories supporting tissue growth and maintenance will be integrated with external and on-chip electro-optofluidic analytical modules to become part of an ecosystem that is modeled after open-source software. It will use commodity sensors, cameras, and computers linked in platforms flexibly designed using simple 3D printing, molding, etching and milling techniques potentially available at any institution. This will stimulate rapid innovation in experimental platforms for tissue culture. We will push this technology and use its best-in-class capabilities to make progress in neurodevelopment and pediatric cancer, addressing big questions. What genes contribute most importantly and specifically to human brain development? How do they go wrong in neurodevelopmental disease or brain injury? What specific molecular pathways are disrupted in individual pediatric cancer cases? How can we test pathway-specific treatments in a tissue model specific to each patient? Our education and outreach plans include a training program to develop a diverse and inclusive cohort of undergraduate students trained in genomic science through secondary school and community college outreach as well as coding workshops and research-based laboratory classes for UCSC undergraduates to develop core competencies. Participation in these activities serve as a basis for training graduate students and postdocs in inclusive pedagogy and mentorship. We are also developing a one-stop information hub to form an online community and to share our technology through immersive webinars and tutorials aimed at a broader audience with a range of expertise from the general public to scientists and clinicians at research institutions. Our work will enable significant advances in neuroscience and cancer research and education, stimulate a new open-source culture in cell biology and genomics, and democratize scientific and educational access beyond elite institutions, extending sharing projects like NHGRI ANvIL beyond data and code to include experiments and Internet-connected experimental platforms.