权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Integrating social and state cues that motivate vocal behavior

整合激发声音行为的社会和国家线索

基本信息

批准号：
10314607
负责人：
Joel Tripp
金额：
$ 6.64万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10314607
关键词：
Address Advertisements Affect Aggressive behavior Androgens Animals Behavior Behavioral Brain Brain region Cell Nucleus Cells Complex Cues Data Dimensions Educational process of instructing Environment Exposure to Faculty Feeding behaviors Fellowship Female Foundations Frequencies Future Genes Genetic Transcription Genomics Glucocorticoids Hormonal Hormones Hypothalamic structure Immunoprecipitation Individual Injections Label Laboratories Laryngeal muscle structure Leptin Literature Mental Health Mental disorders Mentorship Messenger RNA Methods Motivation Mus Neurodevelopmental Disorder Neurons Neurosciences Partner in relationship Personal Satisfaction Population Positioning Attribute Process Proteins Psyche structure RNA Regulation Research Rodent Rodent Model Role Saline Shapes Signaling Molecule Social Behavior Social Controls Social Environment Social Interaction Stress System Testing Texas Tissues Training Transcript Universities Vertebrates Viral Wakefulness Work activity marker adipokines austin brain cell candidate marker career cell type cricothyroid muscle energy balance experience experimental study hormonal signals insight mad itch virus male neural circuit neural model neuromechanism novel paraventricular nucleus physical conditioning programs rabies viral tracing receptor relating to nervous system reproductive response responsible research conduct social social cognition social communication social deficits social engagement tenure track transcriptome sequencing vocal control vocalization

项目摘要

PROJECT SUMMARY Positive social interactions are critical for mental wellness and physical health. Many mental and neurodevelopmental disorders affect or are affected by the motivation to socially engage. This motivation is influenced by a myriad of factors that include external environment, particularly social context, as well as internal factors such as stress, reproductive status, and energetic state. Typically, the influence of these factors on social motivation are studied in isolation; however, we know that outside of the laboratory these factors interact and are integrated with one another. This proposal makes use of a novel system, Alston’s singing mouse (Scotinomys teguina), to investigate the neural circuit integrating social context and one important dimension of internal state: perceived energy balance. Singing mice are an ideal species in which to investigate these questions, as manipulations to social environment (e.g. exposure to potential mates or playback of conspecific song) and hormonal state (e.g. androgens, glucocorticoids, and leptin) can all influence vocal engagement. Through three aims, I will test the hypothesis that cues of social context and energy balance are integrated in specific cell types in the paraventricular hypothalamus. In Aim 1, I will work in the lab of Peter Strick at the University of Pittsburgh, using rabies virus tracing of inputs to laryngeal muscle to identify the brain circuit underlying singing mouse vocalization. This aim will confirm that energetic-sensing nuclei sit upstream of the paraventricular hypothalamus in the singing mouse vocal circuit or allow me to refine my hypothesis and choose a new target nucleus if necessary. Next, I will work in the lab of my sponsor Steven Phelps, at the University of Texas-Austin to identify neurons integrating social context and perceived energetic state. In Aim 2 I will use the neural activation marker, phospho-S6, to test the hypothesis that paraventricular hypothalamus neurons increase activity in response to exposure to females, leptin injections, or both. Then, I will use PhosphoTRAP, a method of sequencing RNA from recently active neurons, to identify transcripts expressed by neurons of the paraventricular hypothalamus that are activated by female exposure, leptin injection, or both. Finally, in Aim 3, I will use single-nucleus RNA-sequencing of the paraventricular hypothalamus to identify cell types in the region and relate these to candidate markers found in Aim 2. This will allow me to determine whether transcripts enriched in active cells correspond to specific cell types. Together, these aims will identify a circuit and specific cell types within it that integrate social and internal cues that influence vocal effort, enhancing our understanding of the complex processes that drive social motivation and laying the groundwork for future studies of social motivation and mammalian vocalization using this novel species. Through this fellowship I will gain not only the technical training described above, but also further training in the responsible conduct of research, teaching, and mentorship that will be invaluable for my future career in a tenure-track faculty position.

项目总结