Allergen T cell epitopes and phenotypes in peanut allergy
Allergen T cell epitopes and phenotypes in peanut allergy
批准号:
10318125
负责人:
William W. Kwok
金额:
$49.93万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-01-01 至 2023-12-31
关键词:
AllergensAllergicAllergic DiseaseAllergy to peanutsAnaphylaxisBiological AssayBiological MarkersCCR6 geneCD4 Positive T LymphocytesCellsClinicalCytometryData AnalysesDevelopmentDiseaseElementsEpitopesEragrostisFlow CytometryFood HypersensitivityFrequenciesFunctional disorderGene ExpressionGene Expression ProfileHelper-Inducer T-LymphocyteHeterogeneityHypersensitivityIgEImmunologyImmunotherapyIndividualKnowledgeLinkMediatingMolecular ProfilingMonitorPathogenesisPhenotypePlayRegulatory T-LymphocyteRoleSpecificityStainsStratificationSubgroupSystemT cell responseT-LymphocyteT-Lymphocyte EpitopesTNFSF5 geneTechnologyTestingTimeUp-Regulationantigen-specific T cellsdisease heterogeneityeffector T cellfunctional disabilityresponsesingle-cell RNA sequencingtranscriptome sequencingtranscriptomics
中文摘要
项目概要 – 项目 1
花生过敏是一种常见的食物过敏,与致命的过敏反应有关。虽然过敏是一种
IgE 介导的疾病,越来越多的证据表明过敏原特异性 CD4 T 细胞发挥着重要作用
在疾病发展中的作用。该项目的目标是将花生过敏建立为异质性
使用花生表位特异性 T 细胞作为生物标志物来定义疾病异质性。具体目标
该项目将包括: 1) 鉴定花生特异性 CD4 T 细胞表位; 2) 检查表型
花生表位特异性T辅助细胞; 3)检验花生特异性T细胞表型的假设
取决于他们识别的表位; 4) 评估花生特异性T辅助细胞的表型异质性
花生过敏受试者的细胞; 5) 检验花生特异性 T 细胞表型可以被
用于将花生过敏受试者分为 2 种不同的亚型; 5) 建立表型之间的联系
花生特异性 T 细胞的分子特征和过敏性疾病状态; 6) 检查表位
特异性花生特异性调节性 T 细胞和 7) 检验调节性 T 细胞在过敏中的假设
受试者有功能障碍。
我们将使用 II 类四聚体来追踪花生特异性细胞和尖端技术,包括 CyTOF
和单细胞 RNA-seq,用于检查花生特异性 T 细胞。 CD154/CD137 检测也将被开发
监测花生特异性 T 效应细胞和调节性 T 细胞。累积的结果将带来很多
更好地了解花生特异性T细胞在花生过敏发病机制中的作用。
英文摘要
Project Summary – Project 1
Peanut allergy is a common food allergy that is associated with fatal anaphylactic reaction. While allergy is an
IgE mediated disease, accumulating evidence suggests that allergen specific CD4+ T cells play a significant
role in disease development. The objective of this project is to establish peanut allergy as a heterogeneous
disease with peanut epitope specific T cells as a biomarker to define disease heterogeneity. The specific aims
of this project will include: 1) Identifying peanut specific CD4+ T cell epitope; 2) Examining the phenotype of
peanut epitope specific T helper cells; 3) Testing the hypothesis that the phenotype of peanut specific T cells
depends on the epitope they recognized; 4) Evaluating phenotype heterogeneity of peanut specific T helper
cells in peanut allergic subjects; 5) Testing the hypothesis that phenotype of peanut specific T cells can be
used to segregate peanut allergic subjects into 2 distinct subtypes; 5) Establishing a link between phenotype
and molecular signature of peanut specific T cells and allergic disease status; 6) Examining the epitope
specificity peanut specific regulatory T cells and 7) Testing the hypothesis that regulatory T cells in allergic
subjects are functionally impaired.
We will use class II tetramers in tracking peanut specific cell and cutting edge technologies, including CyTOF
and single cell RNA-seq, in examining peanut specific T cells. A CD154/CD137 assay will also be developed to
monitor both peanut specific T effectors and regulatory T cells. The cumulative results will lead to a much
better understanding of peanut specific T cells in the pathogenesis of peanut allergy.
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会议论文
Core-001
-
批准号:10187732
-
项目类别:
-
资助金额:$33.27万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes and phenotypes in peanut allergy
-
批准号:10318131
-
项目类别:
-
资助金额:$34.38万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Core-001
-
批准号:10318134
-
项目类别:
-
资助金额:$24.56万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Antigen specific T cell responses to two different strains of SARS-CoV-2
-
批准号:10161553
-
项目类别:
-
资助金额:$64.33万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Project-002
-
批准号:10187731
-
项目类别:
-
资助金额:$31.06万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Project-002
-
批准号:10318133
-
项目类别:
-
资助金额:$22.92万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes and phenotypes in peanut allergy and immunotherapy
-
批准号:10265786
-
项目类别:
-
资助金额:$81.85万
-
财政年份:2020
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes and phenotypes in peanut allergy and immunotherapy
-
批准号:10089383
-
项目类别:
-
资助金额:$101.66万
-
财政年份:2018
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes and phenotypes in peanut allergy and immunotherapy
-
批准号:10318122
-
项目类别:
-
资助金额:$101.66万
-
财政年份:2018
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes during the management of peanut allergic disease
-
批准号:10089401
-
项目类别:
-
资助金额:$2.77万
-
财政年份:2018
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes during the management of peanut allergic disease
-
批准号:10318123
-
项目类别:
-
资助金额:$4.02万
-
财政年份:2018
-
负责人:William W. Kwok
-
依托单位:
Allergen T cell epitopes and phenotypes in peanut allergy
-
批准号:10089404
-
项目类别:
-
资助金额:$33.94万
-
财政年份:2018
-
负责人:William W. Kwok
-
依托单位:
Mechanistic Study of Peanut-specific T cells Pre and Post Oral Immunotherapy
-
批准号:8879293
-
项目类别:
-
资助金额:$42.61万
-
财政年份:2014
-
负责人:William W. Kwok
-
依托单位:
Mechanisms for HLA-DQ mediated disease protection and susceptibility
-
批准号:8436052
-
项目类别:
-
资助金额:$349.12万
-
财政年份:2012
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负责人:William W. Kwok
-
依托单位:
Identifying Epitopes Recognized by Influenza and Flavivirus.
-
批准号:8751045
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项目类别:
-
资助金额:$157.3万
-
财政年份:2009
-
负责人:William W. Kwok
-
依托单位:
Identifying Epitopes Recognized by Influenza and Flavivirus.
-
批准号:8877353
-
项目类别:
-
资助金额:$44.04万
-
财政年份:2009
-
负责人:William W. Kwok
-
依托单位:
Allergen and T-Cell Reagent Resources for the Study of Allergic Diseases
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批准号:8317525
-
项目类别:
-
资助金额:$218.94万
-
财政年份:2007
-
负责人:William W. Kwok
-
依托单位:
Allergen and T-Cell Reagent Resources for the Study of Allergic Diseases
-
批准号:7950571
-
项目类别:
-
资助金额:$105.57万
-
财政年份:2007
-
负责人:William W. Kwok
-
依托单位:
Functional HLA-A*201-peptide and DR0401-peptide microarrays for Type 1 diabetes
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批准号:7224646
-
项目类别:
-
资助金额:$23.93万
-
财政年份:2006
-
负责人:William W. Kwok
-
依托单位:
Functional HLA-A*201-peptide and DR0401-peptide microarrays for Type 1 diabetes
-
批准号:7295808
-
项目类别:
-
资助金额:$25.66万
-
财政年份:2006
-
负责人:William W. Kwok
-
依托单位:
海外基金