Allergen T cell epitopes and phenotypes in peanut allergy and immunotherapy

概述摘要 拟建的中心将对花生过敏患者的过敏原特异性T细胞反应进行机制研究 通过两个协同项目的受试者。第一个项目将研究表型和功能 花生过敏者和耐受者变应原特异性T细胞的特征 花生。第二个项目将调查过敏原的频率、表型和功能的变化- 与免疫治疗相关的特定T细胞。该项目将利用纵向临床样本 来自花生口服免疫疗法的试验和来自花生肽免疫疗法的样本，其中受试者 将接受皮内注射的花生肽疫苗。这两个项目都将利用尖端技术 包括金属标签II类四聚体试剂与细胞外荧光和单细胞RNA-SEQ的技术研究 花生过敏原特异性T细胞。我们中心的基础设施包括两个核心。核心A，行政部门 核心，将监督两个项目的科学进展，促进项目之间的数据集成，以及 负责中心的管理职责和财务管理。核心B，基因表达和 系统免疫学核心将负责RNA-SEQ、流式细胞术和质量的数据分析 还将在数据管理方面与Core A合作。贝纳罗亚研究所拥有最先进的 艺术设施和技术设备，为技术支持和学科招募建立的基础设施， 和合议性环境，以支持建议的研究。

Summary for Overview The proposed Center will perform mechanistic studies of allergen-specific T cell responses in peanut allergic subjects through two synergistic projects. The first project will investigate the phenotypic and functional characteristics of allergen specific T cells in subjects with peanut allergy and subjects that are tolerant to peanut. The second project will investigate changes in the frequency, phenotype, and function of allergen- specific T cells that are associated with immunotherapy. This project will utilize longitudinal clinical samples from a peanut oral immunotherapy trial and samples from a peanut peptide immunotherapy in which subjects will receive intradermal injection of the peanut peptide vaccine. Both projects will utilize cutting edge technologies including metal tag class II tetramer reagents with CyTOF and single cell RNA-seq to study peanut allergen specific T cells. The infrastructure of our Center includes two cores. Core A, the Administrative Core, will oversee scientific progress of the two projects, facilitate data integration between the projects, and provide managerial responsibility and fiscal management for the Center. Core B, the Gene Expression and Systems Immunology Core will be responsible for data analysis for RNA-seq, Flow Cytometry and Mass Cytometry, and will also work with Core A in data management. Benaroya Research Institute has state-of-the- art facilities and technical equipment, established infrastructure for technical support and subject recruitment, and collegial environments to support the research as propose.