Michigan MoTrPAC Chemical Analysis Site (MiCAS)

密歇根大学身体活动分子传感器(MoTrPAC)化学分析站点 (MICAS)旨在与 MoTrPAC财团。这项工作将使用最先进的代谢组学基础设施由 密歇根新陈代谢学会的化学家、光谱学家、计算机科学家和统计学家组成的团队 肥胖中心(MMOC)，这是NIH资助的几项倡议的总括管理结构。米卡斯会的 由对运动科学和代谢组学有广泛知识和经验的研究人员领导 与来自全国各地的科学家合作，部分是通过NIH资助的 项目。MICAS内的分析平台提供了执行各种有针对性的， 对从细胞到人类的不同系统中的代谢物进行非靶向和流量分析。核心调查人员已经 开发并发布了新的技术和技术，以执行代谢组学分析并整合 这些方法与临床研究和其他分子表型方法相结合，以提高对 运动的生物学及其与代谢健康的关系。 MICAS将包含3个核心，这些核心将扩大现有的行政和技术专门知识。这个 行政核心将监督MICAS的运作，包括MMOC的有效利用 基础设施，管理实验室信息管理系统(LIMS)并确保遵守核心 和MoTrPAc授权的SOP。行政核心将负责科学和行政 与NIH和MoTrPAc联盟的互动，特别是与其他化学分析站点的互动。这个 分析核心将在MoTrPAc财团之间开展工作，对各种样品进行分析 这将在项目中产生，根据需要开发新技术，并提供援助和 关于联合体采用的分析工作流程的意见。生物信息学核心将提供 数据管理，包括数据清理、数据缩减、准确性和再现性的质量控制、 在商定的指导方针下缺少特征识别和整合、归一化和内插。 核心将提供数据的初步统计分析，并帮助提供MoTrPAc和相关的 调查人员拥有专业知识和工具，可将数据与来自其他MoTrPAc的数据进行整合 网站，包括跨多个组学平台生成的数据。最后，生物信息学核心将确保 及时将准确和经过验证的数据传输到指定的存储库。

The University of Michigan’s Molecular Transducers of Physical Activity (MoTrPAC) Chemical Analysis Site (MiCAS) is designed to provide large-scale, high-quality metabolomics analysis in collaboration with the MoTrPAC consortium. The effort will be carried out using state-of-the-art metabolomics infrastructure by a team of chemists, spectroscopists, computer scientists and statisticians in the Michigan Metabolomics and Obesity Center (MMOC), the umbrella administrative structure for several NIH-funded initiatives. MiCAS will be led by investigators with extensive knowledge of exercise science and metabolomics and with experience collaborating with scientists from around the country, obtained in part through leadership roles in NIH-funded projects. The analytical platforms within MiCAS provide the ability to perform a wide variety of targeted, untargeted and flux analysis of metabolites in diverse systems from cells to humans. Core investigators have developed and published novel techniques and technologies to perform metabolomics profiling and to integrate these approaches with clinical studies and other molecular phenotyping methods to improve understanding of the biology of exercise and its relationship to metabolic health. MiCAS will contain 3 cores which will expand on the existing administrative and technical expertise. The Administrative Core will oversee the operations of MiCAS, including efficient utilization of the MMOC infrastructure, manage the Laboratory Information Management System (LIMS) and ensure adherence to Core and MoTrPAc-mandated SOP. The Administrative Core will be in charge of the scientific and administrative interactions with the NIH and MoTrPAc consortium, especially the other Chemical Analysis Sites. The Analysis Core will work across the MoTrPAc consortium to carry out analysis of the wide variety of samples that will be generated in the projects, to develop new technologies as needed and to provide assistance and input regarding the analytical workflows adopted by the consortium. The Bioinformatics Core will provide data management including data cleaning, data reduction, quality control for accuracy and reproducibility, missing feature identification and integration, normalization and interpolation under agreed-upon guidelines. The Core will provide initial statistical analysis of the data and help provide MoTrPAc and associated investigators with expertise and tools for integration of the data with data derived from the other MoTrPAc sites, including data generated across multiple ‘omics platforms. Finally, the Bioinformatics Core will ensure timely transfer of accurate and verified data to designated repositories.