Michigan MoTrPAC Chemical Analysis Site (MiCAS)

密歇根 MoTrPAC 化学分析站点 (MiCAS)

• 批准号: 9246812
• 负责人: CHARLES F BURANT
• 金额: $ 7.75万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-08 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9246812
• 关键词: Adherence; Adipocytes; Adipose tissue; Adopted; Analytical Chemistry; Animals; Bioinformatics; Biological; Biological Assay; Biology; Biometry; Cells; Charge; Chemicals; Clinical; Clinical Research; Collaborations; Communities; Computers; Consultations; Country; Data; Data Analyses; Data Collection; Data Element; Data Quality; Databases; Deposition; Elements; Ensure; Exercise; Funding; Goals; Grant; Guidelines; Health; Health Benefit; Human; Institutes; Knowledge; Laboratories; Leadership; Management Information Systems; Manuscripts; Mass Fragmentography; Measures; Metabolic; Metadata; Methodology; Methods; Michigan; Molecular; Molecular Analysis; Obesity; Phase; Phenotype; Physical activity; Plasma; Pre-Clinical Model; Preparation; Procedures; Process; Production; Proteins; Protocols documentation; Publications; Publishing; Quality Control; Recommendation; Recruitment Activity; Reproducibility; Research; Research Design; Research Infrastructure; Research Personnel; Risk; Rodent; Role; Sampling; Sampling Studies; Science; Scientist; Secure; Site; Skeletal Muscle; Specimen; State Interests; Statistical Data Interpretation; Structure; System; Technical Expertise; Techniques; Technology; Testing; Time; Tissue Banks; Tissues; Training; Transducers; United States National Institutes of Health; Universities; Validation; Work; base; biomarker development; clinical phenotype; clinical research site; computerized data processing; data exchange; data integration; data management; data portal; data reduction; data visualization; design; exercise training; experience; human subject; human tissue; improved; insight; interest; member; metabolomics; molecular phenotype; molecular site; multiple omics; new technology; novel; operation; phenotypic data; pre-clinical; preclinical study; prevent; programs; quality assurance; repository; response; sample collection; secondary analysis; small molecule; tool; usability

The University of Michigan’s Molecular Transducers of Physical Activity (MoTrPAC) Chemical Analysis Site (MiCAS) is designed to provide large-scale, high-quality metabolomics analysis in collaboration with the MoTrPAC consortium. The effort will be carried out using state-of-the-art metabolomics infrastructure by a team of chemists, spectroscopists, computer scientists and statisticians in the Michigan Metabolomics and Obesity Center (MMOC), the umbrella administrative structure for several NIH-funded initiatives. MiCAS will be led by investigators with extensive knowledge of exercise science and metabolomics and with experience collaborating with scientists from around the country, obtained in part through leadership roles in NIH-funded projects. The analytical platforms within MiCAS provide the ability to perform a wide variety of targeted, untargeted and flux analysis of metabolites in diverse systems from cells to humans. Core investigators have developed and published novel techniques and technologies to perform metabolomics profiling and to integrate these approaches with clinical studies and other molecular phenotyping methods to improve understanding of the biology of exercise and its relationship to metabolic health. MiCAS will contain 3 cores which will expand on the existing administrative and technical expertise. The Administrative Core will oversee the operations of MiCAS, including efficient utilization of the MMOC infrastructure, manage the Laboratory Information Management System (LIMS) and ensure adherence to Core and MoTrPAc-mandated SOP. The Administrative Core will be in charge of the scientific and administrative interactions with the NIH and MoTrPAc consortium, especially the other Chemical Analysis Sites. The Analysis Core will work across the MoTrPAc consortium to carry out analysis of the wide variety of samples that will be generated in the projects, to develop new technologies as needed and to provide assistance and input regarding the analytical workflows adopted by the consortium. The Bioinformatics Core will provide data management including data cleaning, data reduction, quality control for accuracy and reproducibility, missing feature identification and integration, normalization and interpolation under agreed-upon guidelines. The Core will provide initial statistical analysis of the data and help provide MoTrPAc and associated investigators with expertise and tools for integration of the data with data derived from the other MoTrPAc sites, including data generated across multiple ‘omics platforms. Finally, the Bioinformatics Core will ensure timely transfer of accurate and verified data to designated repositories.

密歇根大学体力活动分子传感器 (MoTrPAC) 化学分析站点 (MiCAS) 旨在与以下机构合作提供大规模、高质量的代谢组学分析 MoTrPAC 联盟。这项工作将由最先进的代谢组学基础设施进行 密歇根代谢组学的化学家、光谱学家、计算机科学家和统计学家团队 肥胖中心 (MMOC)，是 NIH 资助的多项举措的总括管理结构。 MiCAS将 由具有运动科学和代谢组学广泛知识和经验的研究人员领导 与来自全国各地的科学家合作，部分是通过在 NIH 资助的领导角色中获得的 项目。 MiCAS 内的分析平台能够执行各种有针对性的、 对从细胞到人类的不同系统中的代谢物进行非靶向和通量分析。核心调查人员有 开发并发布了进行代谢组学分析和整合的新技术和技术 这些方法与临床研究和其他分子表型分析方法相结合，以提高对 运动的生物学及其与代谢健康的关系。 MiCAS 将包含 3 个核心，将扩展现有的管理和技术专业知识。这 管理核心将监督 MiCAS 的运营，包括 MMOC 的有效利用 基础设施，管理实验室信息管理系统 (LIMS) 并确保遵守核心 和 MoTrPAc 规定的 SOP。行政核心将负责科学和行政 与 NIH 和 MoTrPAc 联盟的互动，特别是其他化学分析站点。这 Analysis Core 将与 MoTrPAc 联盟合作，对各种样品进行分析 将在项目中产生，根据需要开发新技术并提供援助和 有关联盟采用的分析工作流程的意见。生物信息学核心将提供 数据管理，包括数据清理、数据缩减、准确性和再现性的质量控制， 根据商定的准则缺少特征识别和集成、标准化和插值。 核心将提供数据的初步统计分析，并帮助提供 MoTrPAc 和相关的 拥有专业知识和工具的研究人员可以将数据与其他 MoTrPAc 的数据进行整合 网站，包括跨多个“组学”平台生成的数据。最后，生物信息学核心将确保 及时将准确且经过验证的数据传输到指定存储库。