Animal Imaging

摘要 动物成像核心为各个项目提供非侵入性、高分辨率的定量成像- 基于代谢、表型和基因特征的肿瘤及其 微环境，包括人类癌症啮齿动物模型中肿瘤细胞的体内转移。这是 通过监测“直接靶向”探针以及单通道和多通道的表达来完成 使用光学(生物发光和荧光)、放射性核素(PET、SPECT和 放射自显影)、MRI/MRS、CT和US成像。这些非侵入性成像技术长期以来一直是 在MSK建立，用于小动物成像研究，是定量的，因此很容易适应纵向 学习。ZRC vivarum内所有动物成像功能的共同定位提供了增强的 生物安全和功能，并容纳了Core的Focus 120 microPET和Imveon microPET/microCT，Ivis 光谱和光谱CT光学成像系统NanoSPECT/CT Plu和Vevo 2100超声系统。 Ivis 100光学成像系统已升级为Lumina II系统，并保留在 洛克菲勒研究实验室为大楼内的调查人员提供便捷的通道。一种新的 X-RAD 225Cx图像引导生物辐射器，精确地说，是等中心图像(CT)引导的微照射器 对啮齿动物肿瘤和其他结构的治疗性照射为研究人员提供了 在空间精度和剂量传递精确度方面的临床级放射治疗能力。核心的 7.0T 30厘米钻孔布鲁克核磁共振成像和光谱分析系统在ZRC Vivarum内。新型涤纶 插入(由RC2CA14897资助)，最近收到的Core与7T磁铁兼容，并在 当前使用。此外，酷睿拥有11.7T的垂直磁铁，并将很快接受新的9.4T磁铁 (由1S10 OD023669提供资金)，将安装更新的控制台(AV3)，以取代旧的4.7T MR系统) 在这个空间也是如此。核心还为调查人员提供访问关键辅助设备和 服务，如富士胶片BAS-1800II荧光板数字放射自显影系统，数码相机- 配备奥林巴斯B 201电动工作台荧光显微镜和微米HM500M低温恒温器 Microtome和软件，如用于线性和非线性隔室建模的SAAM II，用于体素的pmod- 基于隔室模型/参数成像，以及用于内部放射性核素辐射剂量测定的Olinda。 这一最先进的动物成像核心的科学价值和成本效益由 以下(除其他指标外)：2013-2017年和9年间近500份经同行审查的核心支持出版物 购买的主要仪器由美国国立卫生研究院和其他共享仪器赠款提供资金支持。

ABSTRACT The Animal Imaging Core provides individual projects with non-invasive, high-resolution quantitative imaging- based capabilities for metabolic, phenotypic, and genetic characterization of tumors and their microenvironment, including in vivo trafficking of tumor cells for rodent models of human cancer. This is accomplished by monitoring of “directly-targeting” probes and of the expression of single-and multi-modality reporter genes using optical (bioluminescence and fluorescence), radionuclide (PET, SPECT, and autoradiography), MRI/MRS, CT, and US imaging. These non-invasive imaging techniques have long been established at MSK for small-animal imaging studies, are quantitative and thus readily adaptable to longitudinal studies. Co-localization of all animal imaging capabilites within the ZRC vivarium provides enhanced biosecurity and functionality and houses the Core’s Focus 120 microPET and Imveon microPET/microCT, Ivis Spectrum and Spectrum CT optical imaging systems NanoSPECT/CT Plu, and Vevo 2100 ultrasound system. The Ivis 100 optical imaging system has been upgraded to a Lumina II system and remains on the 12th floor of the Rockefeller Research Laboratory to provide ready access for investigators in that building. A new isocentric, image (CT)-guided microirradiator, the X-RAD 225Cx Image-Guided Biological Irradiator, for precise (down to 1-mm3) therapeutic irradiation of rodent tumors and other structures provides investigators with clinical-grade radiotherapy capability in terms of spatial accuracy and precision of dose delivery. The Core’s 7.0T 30-cm boreBruker NMR imaging and spectroscopy systems is within the ZRC Vivarium. The new PET insert (funded by RC2CA14897), which the Core recently received is compatible with the 7T magnet and is in current use. Additionally, the Core has an 11.7T vertical magnet and will shortly accept a new 9.4T magnet (funded by 1S10 OD023669) with an updated console (AV3) to replace the old 4.7T MR system) to be installed in this space as well. The Core also provides investigators with access to critical ancillary equipment and services such as a Fuji Film BAS-1800II phosphor-plate digital autoradiography system, a digital-camera- equipped Olympus B 201 fluorescence microscope with motorized stage, and a Microm HM500M cryostatic microtome and software such as SAAM II for linear and non-linear compartmental modeling, PMOD for voxel- based compartmental modeling/ parametric imaging, and OLINDA for internal radionuclide radiation dosimetry. The scientific value and cost-effectiveness of this state-of-the-art Animal Imaging Core is documented by the following (among other metrics): nearly 500 peer-reviewed Core-supported publications from 2013-2017 and 9 major instruments purchased with funding support provided by NIH and other Shared Instrumentation grants.