AFS/SERS Saliva-based SARS-CoV-2 Earliest Infection and Antibodies Detection

AFS/SERS 基于唾液的 SARS-CoV-2 最早感染和抗体检测

• 批准号: 10320991
• 负责人: Tony Jun Huang
• 金额: $ 89.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-12-21 至 2023-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10320991
• 关键词: 2019-nCoV; Acute; Antigens; COVID-19; COVID-19 detection; COVID-19 pandemic; COVID-19 patient; COVID-19 testing; Clinical; Collaborations; Communication Programs; Coupled; Data; Data Collection; Data Coordinating Center; Detection; Development; Emergency response; Emerging Technologies; FDA Emergency Use Authorization; Funding; Immunity; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Infection; Label; Patients; Performance; Point of Care Technology; Population; Public Health; RADx Radical; RNA; Raman Spectrum Analysis; SARS-CoV-2 antibody; SARS-CoV-2 immunity; SARS-CoV-2 infection; Saliva; Sampling; Surface; Technology; Testing; United States National Institutes of Health; Validation; Viral Load result; Virion; Virus; antibody detection; antigen detection; base; data sharing; exosome; extracellular; extracellular vesicles; improved; new technology; particle; point of care; research clinical testing; saliva sample; salivary assay

Project Summary/Abstract: This U18 application is responsive to the NIH’s RADx-rad Emergency Responses to the COVID- pandemic for new or non-traditional technologies developed for single extracellular vesicle, exosome and extracellular RNA (exRNA) isolation and analysis and reposition them for detection of SARS-CoV-2. The applicant’s group is a grantee in the NIH Common Fund “Extracellular RNA Communication (ERC)” Program advancing a new and emerging technology of Acoustofluidic Separation (AFS) for label-free, high yield and purity exosomes from biofluids which is coupled to extracellular RNA characterization using Surface Enhanced Raman Spectroscopy (SERS) for single EV identification. This U18 application is to reposition the AFS EV technology and SERS for the non-invasive earliest detection of SARS-CoV-2 in saliva of infected patients. Host immunity to SARS-CoV-2 will also be assessed in the saliva samples, permitting the earliest detection of SARS-CoV-2 infection and host immunity non-invasively in a saliva sample. Five Specific Aims are in place to reposition the saliva-based AFS and SERS technologies, in a 2-year U18 proposal, to test the hypothesis that an integrated multi-parametric non-invasive saliva test for SARS-CoV- 2 infection, viral load and host immunity test demonstrating clinical performance surpassing current saliva-based SARS-CoV-2 EUA tests.

项目概要/摘要： 这个U18应用程序是响应NIH的RADx-rad紧急响应COVID- 针对单个细胞外囊泡、外泌体和 细胞外RNA（exRNA）的分离和分析，并将它们重新定位用于检测SARS-CoV-2。的 申请人的团体是NIH共同基金“细胞外RNA通讯（ERC）”计划的受资助者 提出了一种新兴的声流分离技术（AFS），用于无标记，高产率和纯度 使用表面增强拉曼将来自生物流体的外来体与胞外RNA表征偶联 光谱（Sers）用于单个EV识别。U18的这一应用将重新定位AFS EV技术 和Sers用于感染患者唾液中SARS-CoV-2的非侵入性最早检测。宿主免疫力 SARS-CoV-2也将在唾液样本中进行评估，以便最早检测SARS-CoV-2 感染和宿主免疫性。 五个具体目标是在2年内重新定位基于唾液的AFS和Sers技术 U18提案，以检验综合多参数非侵入性唾液检测SARS-CoV的假设， 2感染、病毒载量和宿主免疫力测试证明临床性能超过当前基于唾液的 SARS-CoV-2 EUA检测。

项目成果

Controlling the Nucleation and Growth of Salt from Bodily Fluid for Enhanced Biosensing Applications.

• DOI: 10.3390/bios13121016
• 发表时间: 2023-12-06
• 期刊: Biosensors

Electrochemical micro-aptasensors for exosome detection based on hybridization chain reaction amplification.

• DOI: 10.1038/s41378-021-00293-8
• 发表时间: 2021
• 期刊: Microsystems & nanoengineering
• 影响因子: 7.9
• 作者: Zhang W;Tian Z;Yang S;Rich J;Zhao S;Klingeborn M;Huang PH;Li Z;Stout A;Murphy Q;Patz E;Zhang S;Liu G;Huang TJ
• 通讯作者: Huang TJ