Optical Molecular Imaging Core

光学分子成像核心

• 批准号: 10323308
• 负责人: GREGORY MATHEW PALMER
• 金额: $ 3.88万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10323308
• 关键词: Accounting; Animal Housing; Animals; Bioluminescence; Budgets; Calendar; Cancer Biology; Cancer Center Support Grant; Cancer Model; Categories; Collaborations; Consult; Data Analyses; Development; Ensure; Equipment; Experimental Designs; Faculty; Financial Support; Fluorescence; Functional Imaging; Funding; Growth; Image; Imaging Device; Imaging technology; Institutes; Journals; Kinetics; Laboratories; Logistics; Maintenance; Malignant Neoplasms; Microscope; Molecular Analysis; Monitor; Operative Surgical Procedures; Optics; Publications; Research; Research Institute; Research Personnel; Resource Sharing; Resources; Services; Site; Solid; System; Techniques; Technology; Therapeutic; Training; Training Support; Transportation; Tumor Biology; Universities; base; bioluminescence imaging; cancer initiation; cost; cost effective; design; fluorescence imaging; foot; imaging capabilities; in vivo; in vivo optical imaging; intravital microscopy; medical schools; member; molecular imaging; operation; optical imaging; outreach; programs; service utilization; spectroscopic imaging; tomography

ABSTRACT – OPTICAL MOLECULAR IMAGING AND ANALYSIS SHARED RESOURCE The Optical Molecular Imaging and Analysis (OMIA) shared resource provides in vivo optical imaging and spectroscopy ranging from whole animal fluorescence and bioluminescence to functional intravital microscopy. OMIA services can be broken down broadly according to the following categories: 1) optical imaging, 2) window chamber surgery training and support, 3) user training in techniques, and 4) scientific guidance on experimental design, data analysis and interpretation. Within optical imaging, two primary technological approaches are in vivo whole animal bioluminescence and fluorescence imaging. These are achieved using a set of commercial imaging devices, namely the IVIS Lumina and Kinetic, as well as FMT2500LX fluorescence tomography system (all from PerkinElmer). A third major technological approach within optical imaging is intravital microscopy. This approach is facilitated through resources developed over many years in the laboratories of the shared resource co-directors. The available resources include a surgical facility and the supplies needed for performing surgeries, as well as microscopes suitable for intravital functional imaging. Together, these three optical imaging capabilities, plus the other expertise, training, and services of OMIA, provide a unique resource for DCI members to study cancer therapeutics and tumor biology. This is a vital shared resource that, in 2018, provided services to 38 investigators, 71% of whom were DCI members, accounting for 74% of total usage, from all 8 DCI Research Programs. DCI members benefit in particular due to the availability of cost offsets that are proportional to the DCI contribution (typically 10-30% based on the service), as well as a wide range of cancer models, which account for the majority of the utilization of these services. The impact of this facility on DCI members is best highlighted by the fact that this shared resource contributed to 83 publications over the project period, 15 of which were in high impact journals (Impact Factor >9), demonstrating the value of services offered by the resource.

光学分子成像和分析共享资源 光学分子成像和分析（OMIA）共享资源提供体内光学成像和 光谱范围从整个动物荧光和生物发光功能活体显微镜。 OMIA服务可大致分为以下几类：1）光学成像，2）窗口 室手术培训和支持，3）用户技术培训，4）实验科学指导 设计、数据分析和解释。 在光学成像中，两种主要的技术方法是体内整个动物生物发光和 荧光成像这些都是使用一套商业成像设备，即IVIS Lumina 和Kinetic，以及FMT 2500 LX荧光断层扫描系统（均来自PerkinElmer）。第三个主要 光学成像内的技术方法是活体显微术。这一方法通过以下方式得到促进： 资源共享共同主任的实验室多年来开发的资源。可用 资源包括手术设施和执行手术所需的用品，以及显微镜 适用于活体功能成像。这三种光学成像能力，加上其他 OMIA的专业知识、培训和服务为DCI成员研究癌症提供了独特的资源 治疗学和肿瘤生物学。 这是一个重要的共享资源，2018年为38名调查人员提供了服务，其中71%是DCI 会员，占总使用量的74%，来自所有8个DCI研究项目。DCI成员受益于 特别是由于与DCI贡献成比例的成本补偿的可用性（通常为10-30% 基于服务），以及广泛的癌症模型，占大多数的利用率 这些服务。这一设施对DCI成员的影响最突出的事实是， 项目期间，资源为83篇出版物做出了贡献，其中15篇发表在高影响力期刊（Impact 系数>9），表明资源提供的服务的价值。