New Methodologies for Connectomics

连接组学的新方法

基本信息

  • 批准号:
    10345620
  • 负责人:
  • 金额:
    $ 13.32万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-01-01 至 2023-12-31
  • 项目状态:
    已结题

项目摘要

PROJECT ABSTRACT The nervous systems of animals are comprised of neurons connected by a large number of synapses. The resulting neural networks underlie animal behavior and contribute to the storage of learned information in many species. In humans, the miswiring of neural networks likely results in disorders of behavior, learning, and thought. For all these reasons, understanding the development, organization, and disruptions in neural circuits is vital. The goal of connectomics is to produce and study the maps of neuronal connections within nervous systems. Connectomic research requires automated image acquisition of brain tissue images that cover large volumes at high magnification to resolve synapses and methods to generate wiring diagrams from these images. But the connectivity map itself is nevertheless not sufficient to explain the brain functions. Additional information, such as the molecular identity of neurons, needs to be extracted from the same nervous system. The primary goal of this proposal is to develop the heavy metal staining of whole mouse brains and other large brain samples for volumetric electron microscopic mapping of a full connectome (Aim1), generate a library of miniaturized protein binders for correlated light and electron microscopic imaging to bridge connectomics with neuronal cell type studies (Aim2), and expand the use of X-ray microscopy in multiplexed brain imaging (Aim3). These studies will lay the foundation for the development of connectomics and establish new paradigms for multimodal imaging. My career goal is to run an academic lab that develops essential methodologies for brain research. The proposed work that focuses on the most urgent needs of connectomics will become a mainstay for my independent research and allow me to integrate the newly acquired knowledge in neurobiology, biochemistry, and microscopy with my interdisciplinary training in chemistry and materials science. The unique environment at Lichtman lab will put me in a privileged position in order to pursue my career aspirations. I have developed a detailed training plan with my mentor, Prof. Jeff Lichtman, to help me transition to independence. I will meet regularly with him to discuss research progress, strategies for grant writing, student mentorship, and lab management. I will oversee the work of a graduate student to practice my mentorship skills. To broaden my scientific network and establish future collaborations, I will present my work in workshops, connectomics meetings, SfN and ACS annual meetings. As a member of the Harvard Department of Molecular and Cellular Biology, I will have access to leaders in neuroscience, molecular biology, cell biology, and biochemistry, as well as cutting-edge core facilities. The BRAIN Initiative Diversity K99/R00 will provide me the funding required to initiate an ambitious research plan to tackle the outstanding challenges surrounding brain studies.
项目摘要

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Xiaotang Lu其他文献

Xiaotang Lu的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Xiaotang Lu', 18)}}的其他基金

New Methodologies for Connectomics
连接组学的新方法
  • 批准号:
    10542794
  • 财政年份:
    2022
  • 资助金额:
    $ 13.32万
  • 项目类别:

相似海外基金

Wireless CMOS device for observing real-time brain activity and animal behavior
用于观察实时大脑活动和动物行为的无线 CMOS 设备
  • 批准号:
    23K06786
  • 财政年份:
    2023
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Animal, Behavior and Tissue Core
动物、行为和组织核心
  • 批准号:
    10496282
  • 财政年份:
    2023
  • 资助金额:
    $ 13.32万
  • 项目类别:
Landscapes of fear in the Anthropocene: Linking predation risk and human disturbance to animal behavior and ecological outcomes
人类世的恐惧景观:将捕食风险和人类干扰与动物行为和生态结果联系起来
  • 批准号:
    RGPIN-2022-03096
  • 财政年份:
    2022
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Discovery Grants Program - Individual
The role of biological interactions in the evolution of animal behavior
生物相互作用在动物行为进化中的作用
  • 批准号:
    RGPIN-2019-06689
  • 财政年份:
    2022
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Discovery Grants Program - Individual
Development of Semi-Supervised Learning Method using Compressed Video for Real-Time Animal Behavior Analysis
使用压缩视频进行实时动物行为分析的半监督学习方法的开发
  • 批准号:
    22H03637
  • 财政年份:
    2022
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Landscapes of fear in the Anthropocene: Linking predation risk and human disturbance to animal behavior and ecological outcomes
人类世的恐惧景观:将捕食风险和人类干扰与动物行为和生态结果联系起来
  • 批准号:
    DGECR-2022-00323
  • 财政年份:
    2022
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Discovery Launch Supplement
Neural and molecular mechanisms of microbe-sensing in the control of animal behavior - Resubmission - 1
微生物传感控制动物行为的神经和分子机制 - 重新提交 - 1
  • 批准号:
    10315486
  • 财政年份:
    2021
  • 资助金额:
    $ 13.32万
  • 项目类别:
Neural and molecular mechanisms of microbe-sensing in the control of animal behavior - Resubmission - 1
微生物传感控制动物行为的神经和分子机制 - 重新提交 - 1
  • 批准号:
    10412977
  • 财政年份:
    2021
  • 资助金额:
    $ 13.32万
  • 项目类别:
REU Site: Animal Behavior in Context
REU 网站:背景下的动物行为
  • 批准号:
    2050311
  • 财政年份:
    2021
  • 资助金额:
    $ 13.32万
  • 项目类别:
    Standard Grant
Molecular recording to predict cell fate decisions and animal behavior
分子记录预测细胞命运决定和动物行为
  • 批准号:
    10260139
  • 财政年份:
    2021
  • 资助金额:
    $ 13.32万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了