Ultra-High Resolution Gas Phase Separation and Analysis Platform for Metabolomics

用于代谢组学的超高分辨率气相分离和分析平台

• 批准号: 10350669
• 负责人: Yehia Ibrahim
• 金额: $ 63.73万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10350669
• 关键词: Address; Biological Markers; Biomedical Research; Calibration; Chemicals; Chromatography; Clinic; Data; Dependence; Detection; Development; Disease; Dissociation; Foundations; Gases; Informatics; Ions; Isomerism; Length; Liquid Chromatography; Mass Spectrum Analysis; Measurement; Phase; Phenotype; Population; Predictive Value; Reference Standards; Reproducibility; Research; Resolution; Shapes; Speed; Structure; Technology; base; biological systems; clinical application; data acquisition; data management; data quality; experience; improved; informatics tool; ion mobility; metabolome; metabolomics; novel; operation; precision medicine; prototype; research and development; ultra high resolution

PROJECT SUMMARY/ABSTRACT Metabolomics is increasingly utilized and essential to biomedical research, with broad applications extending to the clinic. This potential is inhibited by the limitations of current platforms that are largely based upon either the combination of chromatography separations with mass spectrometry (MS) or by MS alone. The objective of this project is to develop a new ultra-high resolution and ultra-sensitive platform that will enable much higher quality and more comprehensive metabolomics measurements than previously feasible and to do so with significant gains in measurement throughput. The key developments will involve the implementation of greatly improved ion mobility (IM) separations providing much greater resolution, higher speed, broader applicability to metabolite chemical classes, and improved sensitivity and measurement dynamic range in conjunction with mass spectrometry (MS). The new IM-MS platform development efforts will include the essential platform control and data acquisition capabilities and result in a platform ready for initial metabolomics applications, as well as a foundation for its rapid dissemination.

项目摘要/摘要 代谢组学在生物医学研究中的应用越来越广泛 延伸到诊所。这种潜力受到当前平台的限制，这些平台在很大程度上 基于层析分离与质谱仪(MS)的组合或 仅由MS提供。本项目的目标是开发一种新型的超高分辨率和超灵敏 将实现更高质量和更全面的代谢组学测量的平台 比以前更可行，并且在测量吞吐量方面有显著的收益。钥匙 发展将涉及实施大大改进的离子迁移率(IM)分离 为代谢物化学类别提供更高的分辨率、更快的速度和更广泛的适用性， 并结合质谱学改进了灵敏度和测量动态范围 (毫秒)。新的IM-MS平台开发工作将包括基本的平台控制和 数据采集能力，并为初始代谢组学应用做好准备的平台，如 也是其迅速传播的基础。

项目成果

A Miniature Multilevel Structures for Lossless Ion Manipulations Ion Mobility Spectrometer with Wide Mobility Range Separation Capabilities.

• DOI: 10.1021/acs.analchem.1c04700
• 发表时间: 2022-02-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Hollerbach, Adam L.;Norheim, Randolph, V;Kwantwi-Barima, Pearl;Smith, Richard D.;Ibrahim, Yehia M.
• 通讯作者: Ibrahim, Yehia M.

Evaluating Ion Accumulation and Storage in Traveling Wave Based Structures for Lossless Ion Manipulations.

评估基于行波的结构中的离子积累和存储，以实现无损离子操作。

• DOI: 10.1021/jasms.3c00348
• 发表时间: 2023
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Kwantwi-Barima,Pearl;Garimella,SandilyaVB;Attah,IsaacK;Ibrahim,YehiaM
• 通讯作者: Ibrahim,YehiaM

Improving Signal to Noise Ratios in Ion Mobility Spectrometry and Structures for Lossless Ion Manipulations (SLIM) using a High Dynamic Range Analog-to-Digital Converter.

• DOI: 10.1021/jasms.1c00226
• 发表时间: 2021-11-03
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Hollerbach AL;Giberson CM;Lee JY;Huntley AP;Smith RD;Ibrahim YM
• 通讯作者: Ibrahim YM