Biospecimen procurement and tissue microarray manufacture for the CHTN

CHTN 的生物样本采购和组织微阵列制造

• 批准号: 10360587
• 负责人: Christopher A. Moskaluk
• 金额: $ 94.85万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10360587
• 关键词: Adult; African American; Blood; Bone Marrow; Cell Survival; Chemicals; Clinic; Clinical Data; Clinical Informatics; Code; Collection; Consent; Cooperative Human Tissue Network; DNA; Data; Databases; Diagnosis; Diagnostic tests; Disease; Freezing; Funding; Genetic; Genetic Research; Geographic Locations; Geography; Health Service Area; Hispanic; Histologic; Histology; Human; Informatics; Infrastructure; Institution; International Statistical Classification of Diseases and Related Health Problems, Tenth Revision (ICD-10); Leukocytes; Link; Logical Observation Identifiers Names and Codes; Malignant Neoplasms; Malignant neoplasm of lung; Malignant neoplasm of prostate; Maps; Marketing; Medical; Medical Research; Microscopy; Modeling; NCI-Designated Cancer Center; New Mexico; Normal tissue morphology; Nucleic Acids; Paraffin Embedding; Pathologist; Patient Recruitments; Patients; Population; Population Heterogeneity; Procedures; Quality Control; RNA; Research Personnel; Resources; SNOMED Clinical Terms; Sampling; Scientist; Services; Ships; Site; South Carolina; Specimen; Specimen Handling; Surveys; Systematized Nomenclature of Medicine; Terminology; Time; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Universities; Urine; Virginia; base; biobank; clinical data repository; clinical diagnostics; cohort; data modeling; data sharing; data standards; data structure; demographics; digital; ethnic diversity; human disease; human subject; human tissue; informatics infrastructure; novel diagnostics; novel therapeutics; online resource; prospective; racial and ethnic; recruit; tumor registry; virtual

The University of Virginia (UVA) currently supports the “adult” Mid-Atlantic Division of the Cooperative Human Tissue Network (CHTN), offering high quality human tissue and biofluid specimens procured, stored and shipped under optimized standard operating procedures. A team of consent staff recruit subjects at UVA clinics, with the consent including provisions for genetic sequencing and data sharing as well as donating tissue, blood and bone marrow samples. Staffing, procedures & infrastructure are in place to minimize procurement times and tissue samples are quality controlled by histologic examination by Board-certified Pathologists. Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin- embedded and viable form. We propose to continue this activity, but to expand our current geographic area and to offer biospecimens from a more diverse population of donors. To do so, we have partnered with two other recruitment/procurement sites specifically to augment specimen procurement as well as provide geographic and racial/ethnic diversity. UVA will be joined in biospecimen procurement by the Medical University of South Carolina (MUSC), which has a significantly higher percentage of African-American patients than UVA, and by the University of New Mexico (UNM), which has a significantly higher percentage of Hispanic patients than UVA. As one of the few current CHTN divisions able to procure fresh and frozen samples of prostate cancer, we propose to continue to offer this precious resource to CHTN investigators. We also propose to continue our focus on constructing tissue microarrays (TMAs) for the CHTN, to help supplement the traditional strengths of the CHTN (prospective customizable procurement of tissue) with larger cohorts of specimens in TMA format, with more rich annotation of clinical data. Our ability to provide annotated data is enhanced by integration with a digital Clinical Data Repository at UVA and with an established tumor registry. With noted expertise in clinical informatics, we propose to enhance the current CHTN data structure with mapping to standard clinical diagnostic data models (LOINC, SNOMED) and to enhance biorepository-specific data capture with developing data models (BRISQ, SPREC, OBIB, etc.). To further augment biospecimens available to CHTN investigators, we have the opportunity to provide a large legacy collection of lung cancer biospecimens from the Lung Cancer Biospecimen Resource Network. We propose to expand histology guided- macrodissection services to create value-added target tissue enrichment and histologic quality control. We will also provide viable freezing of dissociated tissue and isolated white blood cells, as well as DNA/RNA isolation from tissue specimens.

弗吉尼亚大学 (UVA) 目前支持人类合作组织的“成人”中大西洋分部 组织网络 (CHTN)，提供采购、储存和储存的高质量人体组织和生物体液样本 按照优化的标准操作程序发货。一支同意工作人员团队在 UVA 招募受试者 诊所，并征得同意，包括基因测序和数据共享以及捐赠的规定 组织、血液和骨髓样本。人员配备、程序和基础设施已到位，以最大程度地减少 采购时间和组织样本均由委员会认证的组织学检查进行质量控制 病理学家。采购的组织标本以新鲜冷冻、化学固定、石蜡形式提供。 嵌入式和可行的形式。我们建议继续这项活动，但扩大我们当前的地理区域 并提供来自更多样化的捐赠者群体的生物样本。为此，我们与两家公司合作 其他专门用于增加样本采购并提供样本的招聘/采购网站 地理和种族/族裔多样性。 UVA 将加入医疗机构的生物样本采购 南卡罗来纳大学 (MUSC)，该校非裔美国患者的比例明显更高 与 UVA 相比，新墨西哥大学 (UNM) 的西班牙裔比例明显更高 患者比UVA。作为目前为数不多的能够采购新鲜和冷冻样品的 CHTN 部门之一 前列腺癌，我们建议继续向 CHTN 研究人员提供这一宝贵资源。我们也 建议继续专注于为 CHTN 构建组织微阵列 (TMA)，以帮助补充 CHTN（前瞻性可定制组织采购）的传统优势，拥有更多的 TMA格式的标本，具有更丰富的临床数据注释。我们提供带注释数据的能力是 通过与 UVA 的数字临床数据存储库以及已建立的肿瘤登记处的集成而得到增强。 凭借临床信息学方面的著名专业知识，我们建议通过以下方式增强当前的 CHTN 数据结构： 映射到标准临床诊断数据模型（LOINC、SNOMED）并增强生物样本库的特定性 通过开发数据模型（BRISQ、SPREC、OBIB 等）捕获数据。进一步扩大生物样本 可供 CHTN 研究人员使用，我们有机会提供大量肺癌遗留资料 来自肺癌生物样本资源网络的生物样本。我们建议扩大组织学指导 宏观解剖服务可创造增值目标组织富集和组织学质量控制。我们将 还提供游离组织和分离白细胞的可行冷冻，以及 DNA/RNA 分离 来自组织标本。