Biospecimen procurement and tissue microarray manufacture for the CHTN

描述(由申请人提供)：目标是根据NCI生物信息库最佳实践，为协作人体组织网络(CHTN)的用户提供高质量的人体组织和生物液样本。人员配备、程序和基础设施到位，以最大限度地减少采购时间。组织样本通过组织学检查进行质量控制，分子评估包括在质量保证计划中。弗吉尼亚大学的标本采购将通过收集东弗吉尼亚医学院和南卡罗来纳医科大学的标本来扩大。私人健康信息按照共同规则和HIPAA规定处理。为保护受试者隐私，所有样本只用一个代码号标识(“去标识”)，链接代码保存在安全数据库中。采购的组织标本以新鲜冷冻、化学固定、石蜡包埋和可存活的形式提供。活组织的选择包括解体固体组织、小组织碎片和从腹水中获得的活细胞的冷冻细胞悬液。除了石蜡包埋，还提供石蜡包埋或冰冻组织的组织切片。组织学指导的大体解剖服务可用于需要丰富细胞群体的研究人员。对于大块和大体解剖的组织样本，都提供核酸提取(RNA和DNA)。目前设在我们机构的CHTN组织微阵列(TMA)倡议将继续得到支持，并将得到扩大。我们提供注释数据的能力通过与弗吉尼亚大学的数字临床数据存储库和已建立的肿瘤登记系统的集成而得到增强。在授权期内，我们部门的成员将合作整合当地的信息资源和集中的CHTN信息资源，并达到NCI规定的生物信息标准。我们部门的成员将通过参与协调会议和科学会议的营销工作来支持CHTN的整体努力。CHTN的改进将通过参与组织质量管理实践并在整合新的技术和生物信息进步方面发挥领导作用。

DESCRIPTION (provided by applicant): The objectives are to provide users of the Collaborative Human Tissue Network (CHTN) with high quality human tissue and biofluid samples in accordance with NCI Best Practices for biorepositories. Staffing, procedures & infrastructure are in place to minimize procurement times. Tissue samples are quality controlled by histologic examination, with molecular assessment included in a quality assurance program. Specimen procurement at The University of Virginia will be augmented by collection at Eastern Virginia Medical School and the Medical University of South Carolina. Private health information is handled in accordance with Common Rule and HIPAA regulations. To protect subject privacy, all samples are identified only by a code number ("de-identified") and linking codes are maintained in secure databases. Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin-embedded and viable form. Options for viable tissue include frozen cell suspensions of disaggregated solid tissue, small tissue fragments, and viable cells obtained from ascites fluid. In addition to paraffin embedding, histologic sections of paraffn embedded or frozen tissue are offered. Histology-guided macrodissection services are available to investigators who require enriched cell populations. Nucleic acid extraction (RNA and DNA) are offered for both bulk and macrodissected tissue samples. The CHTN tissue microarray (TMA) initiative, currently based in our institution, will continue to be supported and will be expanded. Our ability to provide annotated data is enhanced by integration with a digital Clinical Data Repository at The University of Virginia and with an established tumor registry. During the grant period members of our division will cooperate to integrate local informatic resources with centralized CHTN informatic resources, and to achieve compliance with NCI-mandated bioinformatic standards. Members of our division will support the overall efforts of the CHTN by participating in coordinating meetings and in marketing efforts at scientific meetings. Improvement of the CHTN will be provided by participating in organizational quality management practices and offering leadership in integrating new technological and bio-informatic advances.