Biospecimen procurement and tissue microarray manufacture for the CHTN

CHTN 的生物样本采购和组织微阵列制造

• 批准号: 9031568
• 负责人: Christopher A. Moskaluk
• 金额: $ 59.73万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-24 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9031568
• 关键词: Area; Ascites; Bioinformatics; Blood; Cells; Clinical Data; Code; Collection; DNA; Data; Databases; Diagnostic tests; Disease; Freezing; Grant; Health; Health Insurance Portability and Accountability Act; Histologic; Histology; Human; Institution; Leadership; Link; Liquid substance; Malignant Neoplasms; Marketing; Measures; Medical; Microscopy; Molecular; Normal tissue morphology; Nucleic Acids; Online Systems; Paraffin Embedding; Population; Practice Management; Privacy; Procedures; Quality Control; RNA; Regulation; Research Infrastructure; Research Personnel; Resource Informatics; Resources; Sampling; Scientist; Secure; Services; Site; Solid; South Carolina; Specimen; Surveys; Suspension substance; Suspensions; Time; Tissue Microarray; Tissue Sample; Tissues; Universities; Urine; Virginia; base; biobank; common rule; digital; human disease; human subject; human tissue; informatics infrastructure; medical schools; meetings; member; novel diagnostics; novel therapeutics; programs; quality assurance; tissue/cell preparation; tumor registry; virtual

DESCRIPTION (provided by applicant): The objectives are to provide users of the Collaborative Human Tissue Network (CHTN) with high quality human tissue and biofluid samples in accordance with NCI Best Practices for biorepositories. Staffing, procedures & infrastructure are in place to minimize procurement times. Tissue samples are quality controlled by histologic examination, with molecular assessment included in a quality assurance program. Specimen procurement at The University of Virginia will be augmented by collection at Eastern Virginia Medical School and the Medical University of South Carolina. Private health information is handled in accordance with Common Rule and HIPAA regulations. To protect subject privacy, all samples are identified only by a code number ("de-identified") and linking codes are maintained in secure databases. Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin-embedded and viable form. Options for viable tissue include frozen cell suspensions of disaggregated solid tissue, small tissue fragments, and viable cells obtained from ascites fluid. In addition to paraffin embedding, histologic sections of paraffn embedded or frozen tissue are offered. Histology-guided macrodissection services are available to investigators who require enriched cell populations. Nucleic acid extraction (RNA and DNA) are offered for both bulk and macrodissected tissue samples. The CHTN tissue microarray (TMA) initiative, currently based in our institution, will continue to be supported and will be expanded. Our ability to provide annotated data is enhanced by integration with a digital Clinical Data Repository at The University of Virginia and with an established tumor registry. During the grant period members of our division will cooperate to integrate local informatic resources with centralized CHTN informatic resources, and to achieve compliance with NCI-mandated bioinformatic standards. Members of our division will support the overall efforts of the CHTN by participating in coordinating meetings and in marketing efforts at scientific meetings. Improvement of the CHTN will be provided by participating in organizational quality management practices and offering leadership in integrating new technological and bio-informatic advances.

描述（由申请人提供）：目的是根据NCI生物储存库最佳实践，为协作人体组织网络（CHTN）的用户提供高质量的人体组织和生物流体样本。 人员配备、程序和基础设施到位，以最大限度地缩短采购时间。 组织样本通过组织学检查进行质量控制，分子评估包括在质量保证计划中。 弗吉尼亚大学的标本采购将通过东弗吉尼亚医学院和南卡罗来纳州医科大学的采集来增加。 私人健康信息按照通用规则和HIPAA法规处理。为保护受试者隐私，所有样本仅通过代码编号识别（“去识别”），链接代码保存在安全数据库中。采购的组织标本以新鲜冷冻、化学固定、石蜡包埋和活的形式提供。活组织的选择包括分解的实体组织的冷冻细胞悬浮液、小组织碎片和从腹水中获得的活细胞。除石蜡包埋外，还提供石蜡包埋或冷冻组织的组织切片。组织学引导的宏观解剖服务可用于需要富集细胞群的研究者。核酸提取（RNA和DNA）可用于散装和宏观解剖组织样本。CHTN组织微阵列（TMA）倡议，目前基于我们的机构，将继续得到支持，并将扩大。我们提供注释数据的能力通过与弗吉尼亚大学的数字临床数据库和已建立的肿瘤登记处的集成而得到增强。在资助期间，我们部门的成员将合作整合当地的信息资源与集中的CHTN信息资源，并实现符合NCI规定的生物信息标准。我们部门的成员将通过参加协调会议和科学会议的营销工作来支持CHTN的整体努力。CHTN的改进将通过参与组织质量管理实践和在整合新技术和生物信息学进步方面提供领导来提供。