Molecular characterization of a human salivary gland tumor xenograft bank

人唾液腺肿瘤异种移植库的分子特征

• 批准号: 7837342
• 负责人: Christopher A. Moskaluk
• 金额: $ 50万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-17 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7837342
• 关键词: Address; Adenoid Cystic Carcinoma; Area; Bioinformatics; Biological Models; Cell Line; Clinical; Communities; Cooperative Human Tissue Network; Cytogenetics; Data; Data Analyses; Freezing; Future; Gene Expression Profiling; Genome; Genomics; Goals; Human; Immunodeficient Mouse; Investigation; Laboratories; Liquid Chromatography; Michigan; Microarray Analysis; Modeling; Molecular; Molecular Profiling; Mus; Patients; Phosphopeptides; Phosphorylation; Preclinical Testing; Proteomics; Protocols documentation; Quality Control; Reagent; Registries; Research; Research Infrastructure; Research Personnel; Resource Sharing; Resources; Salivary; Salivary Gland Neoplasms; Sampling; Science; Source; Testing; Therapeutic; Tissue Microarray; Tissue Procurements; Tissue Sample; Tissues; Transcript; Translational Research; Tumor Biology; Tumor Tissue; Universities; Virginia; Work; Xenograft Model; Xenograft procedure; base; biobank; cohort; comparative genomic hybridization; implantation; in vivo; mouse model; programs; public health relevance; research study; tumor; tumor xenograft; virtual; web site

DESCRIPTION (provided by applicant): This application addresses broad Challenge Area (15) Translational Science and specific Challenge Topic, 15-DE-101 Molecular Profiling and Developing Mouse Models for Salivary Gland Tumor Research. The proposed work will expand an ongoing successful research program of creating human salivary adenoid cystic carcinoma tumor xenografts in immunodeficient mice to include the other common and clinically aggressive human salivary gland tumor types. The investigators will utilize the resources of the University of Virginia Biorepository, the University of Michigan Tissue Procurement Facility, the NIDCR-sponsored Salivary Gland Tumor Biorepository, the NCI-sponsored Cooperative Human Tissue Network and the Adenoid Cystic Carcinoma Registry to collect viable tumor samples for xenograft implantation. Resulting xenografts will be characterized on the genomic and proteomic level (cytogenetics, array CGH, microarray gene expression profiling, mass spectroscopic analysis of protein phosphorylation). The raw data from these analyses will be made available on a publicly-available website. Special emphasis will be given to creating a stock of viably frozen tissue and creating tissue microarrays of tissue samples from the model, thus creating virtual and real salivary gland tumor resources that investigators may query to create hypotheses of salivary gland tumor biology, and request materials to test those hypotheses. The use of these xenograft model systems include providing raw material for discovery science, a source to derive cell culture lines, reagents for in vivo testing of hypotheses related to salivary gland tumor biology and reagents for preclinical testing of therapeutic strategies for these tumor types. PUBLIC HEALTH RELEVANCE: The study of human salivary gland tumors is hampered by a lack of model systems and tumor tissue to study. We propose to continuously grow salivary gland tumors obtained from patients in mice, which can be used in experiments to understand the molecular basis of these tumors and to test new forms of therapy.

描述（由申请人提供）：本申请涉及广泛的挑战领域 (15) 转化科学和具体挑战主题，15-DE-101 分子分析和开发用于唾液腺肿瘤研究的小鼠模型。拟议的工作将扩大正在进行的成功研究计划，即在免疫缺陷小鼠中创建人类唾液腺样囊性癌肿瘤异种移植物，以包括其他常见和临床上侵袭性的人类唾液腺肿瘤类型。研究人员将利用弗吉尼亚大学生物样本库、密歇根大学组织采购设施、NIDCR 资助的唾液腺肿瘤生物样本库、NCI 资助的合作人体组织网络和腺样囊性癌登记处的资源来收集用于异种移植的可行肿瘤样本。所得异种移植物将在基因组和蛋白质组水平上进行表征（细胞遗传学、阵列 CGH、微阵列基因表达谱、蛋白质磷酸化的质谱分析）。这些分析的原始数据将在公开网站上提供。将特别强调创建活的冷冻组织库存并从模型中创建组织样本的组织微阵列，从而创建虚拟和真实的唾液腺肿瘤资源，研究人员可以查询这些资源以创建唾液腺肿瘤生物学的假设，并索取材料来检验这些假设。这些异种移植模型系统的使用包括为发现科学提供原材料、衍生细胞培养系的来源、用于体内测试与唾液腺肿瘤生物学相关的假设的试剂以及用于这些肿瘤类型的治疗策略的临床前测试的试剂。 公共健康相关性：人类唾液腺肿瘤的研究因缺乏模型系统和肿瘤组织而受到阻碍。我们建议持续培养从小鼠患者身上获得的唾液腺肿瘤，可用于实验以了解这些肿瘤的分子基础并测试新的治疗形式。