Tissue procurement & processing for molecular analysis

描述（由申请人提供）：本提案的目标是继续中大西洋分部的努力，根据nci支持的生物储存库的第一代指南，为合作人体组织网络的用户提供高质量的人体组织和生物流体样本。组织样品通过组织学检查进行质量控制，生物标本的分子评估包括在质量控制程序中。在可能的情况下，将征求患者的知情同意，并按照已批准的放弃同意指南的指示，制定匿名化生物标本的程序。根据通用规则和HIPAA法规处理私人健康信息。为了保护受试者的隐私，所有样本仅通过代码编号进行识别（“去识别”），链接代码保存在安全的数据库中。获得的组织标本以新鲜冷冻，化学固定，石蜡包埋和活的形式提供。活组织的选择包括从机械和酶解的固体组织中获得的冷冻细胞悬液，小组织碎片的冷冻细胞悬液，从淋巴结中分解的淋巴细胞的冷冻细胞悬液和从腹水中获得的活细胞的冷冻细胞悬液。除了石蜡包埋，石蜡包埋或冷冻组织的组织学切片提供。组织学引导的宏观解剖服务是提供给调查者谁需要丰富的细胞群。核酸提取（RNA和DNA）将提供大体积和大解剖组织样本。主要设在中大西洋分部的CHTN组织微阵列（TMA）计划将继续得到支持并将扩大。通过与弗吉尼亚大学的数字临床数据存储库和已建立的肿瘤登记处集成，我们机构提供注释数据的能力将得到增强。在资助期间，我们部门的成员将合作整合当地信息资源与集中的CHTN信息资源，并达到caBIG或其他nci规定的生物信息学标准。中大西洋分部的成员将通过参与科学会议的协调会议和营销工作来支持CHTN的整体工作。通过参与组织质量管理实践和在整合新技术和生物信息学进步方面发挥领导作用，将提供CHTN的改进。

DESCRIPTION (provided by applicant): The objectives of this proposal are to continue the efforts of the Mid-Atlantic division to provide users of the Cooperative Human Tissue Network with high quality human tissue and biofluid samples in accordance with the first generation guidelines for NCI-supported biorepositories. Tissue samples are quality controlled by histologic examination, with molecular assessment of biospecimens included in quality control procedures. Informed patient consent will be sought when possible and procedures are in place to anonymize biospecimens as indicated by approved waiver of consent guidelines. Private health information is handled in accordance with Common Rule and HIPAA regulations. To protect subject privacy, all samples are identified only by a code number ("de-identified") and linking codes are maintained in secure databases. Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin-embedded and viable form. Options for viable tissue include frozen cell suspensions from mechanically and enzymatically disaggregated solid tissue, frozen cell suspensions of small tissue fragments, frozen cell suspensions of lymphocytes disaggregated from lymph nodes and frozen cell suspensions of viable cells obtained from ascites fluid. In addition to paraffin embedding, histologic sections of paraffin embedded or frozen tissue are offered. Histology-guided macrodissection services are available to investigators who require enriched cell populations. Nucleic acid extraction (RNA and DNA) will be offered for both bulk and macrodissected tissue samples. The CHTN tissue microarray (TMA) initiative, which is predominantly based at the Mid-Atlantic division, will continue to be supported and will be expanded. The ability of our institution to provide annotated data will be enhanced by integrating with a digital clinical data repository at The University of Virginia and with an established tumor registry. During the grant period members of our division will cooperate to integrate local informatic resources with centralized CHTN informatic resources, and to achieve compliance with caBIG, or other NCI-mandated bioinformatic standards. Members of the Mid-Atlantic division will support the overall efforts of the CHTN by participating in coordinating meetings and in marketing efforts at scientific meetings. Improvement of the CHTN will be provided by participating in organizational quality management practices and offering leadership in integrating new technological and bio- informatic advances.