Tissue procurement and processing for molecular analysis

{\fnSimHei\bord1\shad1\pos（200，288）} {\*\generator Msftedit 5.41.15.1515;}本提案的目标是继续中大西洋分部的努力，为用户提供 合作人体组织网络，提供符合\par标准的高质量人体组织和生物流体样本 NCI支持的生物储存库的第一代指南。组织样本由\par进行质量控制 组织学检查，生物标本的分子评估包括在质量控制程序中。par 在可能的情况下，将征求患者的知情同意，并制定程序以匿名\par 批准的同意豁免指南所示的生物标本。处理私人健康信息\par 根据通用规则和HIPAA法规。为保护受试者隐私，所有样本均\par 仅通过代码编号进行识别（“取消识别”），链接代码保存在安全数据库中。\ par 采购的组织标本以新鲜冷冻、化学固定、石蜡包埋和\par 可行的形式活组织的选择包括机械和酶促分离的冷冻细胞悬液， 分解的实体组织、小组织碎片的冷冻细胞悬浮液、 从淋巴结中分离的淋巴细胞和从淋巴结中获得的活细胞的冷冻细胞悬浮液 腹水除了石蜡包埋，石蜡包埋或冷冻组织的组织学切片也可用于 提供。组织学引导的宏观解剖服务可用于需要富集细胞的研究者。 人口。核酸提取（RNA和DNA）将提供给散装和宏观解剖组织\par 样品CHTN组织微阵列（TMA）计划主要位于中大西洋地区， 将继续支持和扩大该司的工作。我们的机构提供注释\par的能力 数据将通过与弗吉尼亚大学的数字临床数据库集成来增强， 有肿瘤登记的人在赠款期间，我们部门的成员将合作\par 将本地信息资源与集中的CHTN信息资源相结合，并实现合规性\p 与caBIG或其他NCI-mandated生物信息学标准。中大西洋分部的成员将支持\par CHTN通过参加协调会议和在科学领域的营销努力， 会议. CHTN的改进将通过参与组织质量管理来提供， 实践并在整合新技术和生物信息学进步方面发挥领导作用。fs20\par \par }

{\rtf1\ansi\ansicpg1252\deff0\deflang1033{\fonttbl{\f0\fswiss\fcharset0 Arial;}} {\*\generator Msftedit 5.41.15.1515;}\viewkind4\uc1\pard\f0\fs19 The objectives of this proposal are to continue the efforts of the Mid-Atlantic division to provide users of the\par Cooperative Human Tissue Network with high quality human tissue and biofluid samples in accordance with\par the first generation guidelines for NCI-supported biorepositories. Tissue samples are quality controlled by\par histologic examination, with molecular assessment of biospecimens included in quality control procedures.\par Informed patient consent will be sought when possible and procedures are in place to anonymize\par biospecimens as indicated by approved waiver of consent guidelines. Private heath information is handeled\par in accordance with Common Rule and HIPAA regulations. To protect subject privacy, all samples are\par identified only by a code number ("de-identified") and linking codes are maintained in secure databases.\par Procured tissue specimens are made available in fresh-frozen, chemically-fixed, paraffin-embedded and\par viable form. Options for viable tissue include frozen cell suspensions from mechanically and enzymatically\par disaggregated solid tissue, frozen cell suspensions of small tissue fragments, frozen cell suspensions of\par lymphocytes disaggregated from lymph nodes and frozen cell suspensions of viable cells obtained from\par ascites fluid. In addition to paraffin embedding, histologic sections of paraffin embedded or frozen tissue are\par offered. Histology-guided macrodissection services are available to investigators who require enriched cell\par populations. Nucleic acid extraction (RNA and DNA) will be offered for both bulk and macrodissected tissue\par samples. The CHTN tissue microarray (TMA) initiative, which is predominantly based at the Mid-Atlantic\par division, will continue to be supported and will be expanded. The ability of our institution to provide annotated\par data will be enhanced by integrating with a digital clinical data repository at The University of Virginia and\par with an established tumor registry. During the grant period members of our division will cooperate to\par integrate local informatic resources with centralized CHTN informatic resources, and to achieve compliance\par with caBIG, or other NCI-mandated bioinformatic standards. Members of the Mid-Atlantic division will support\par the overall efforts of the CHTN by participating in coordinating meetings and in marketing efforts at scientific\par meetings. Improvement of the CHTN will be provided by partcipating in organizational quality management\par practices and offering leadership in integrating new technological and bio- informatic advances.\fs20\par \par }