权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Coordinated regulation of developmental transition by protein and long noncoding RNA components

蛋白质和长链非编码RNA成分对发育转变的协调调控

基本信息

批准号：
10367472
负责人：
Sibum Sung
金额：
$ 32.11万
依托单位：
UNIVERSITY OF TEXAS AT AUSTIN
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10367472
关键词：
26S proteasome 3-Dimensional Address Affect Animals Arabidopsis Architecture Binding Proteins Biological Biological Models Cell Nucleus Chromatin Chromatin Loop Chromatin Structure Complex Cues Data Development Drosophila genus Environment Epigenetic Process Eukaryota Eukaryotic Cell Event Experimental Models Gene Expression Gene Expression Regulation Genes Genetic Transcription Genome Goals Human Knowledge Light Mediating Modeling Molecular Conformation Nature Nucleosomes Organism Phenotype Photoreceptors Plant Roots Plants Play Polycomb Process Proteins Regulation Regulator Genes Repression Research Role SPT6 Protein Signal Transduction Stimulus Structure Study models System Temperature Testing Ubiquitin Untranslated RNA Work Yeasts base bioinformatics pipeline chromatin modification chromatin protein design epigenetic regulation experimental study genetic regulatory protein genome-wide high throughput analysis histone modification in vivo phyB phytochrome predictive modeling programs protein degradation response three dimensional structure

项目摘要

Coordinated regulation of developmental transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable organisms to adapt to developmental and environmental cues. Plants have evolved intricate regulatory networks to control development in response environmental stimuli such as temperature and light. For example, prolonged cold triggers vernalization, a process that accelerates flowering through epigenetic changes in genes involved in development. Therefore, the vernalization response in Arabidopsis is an excellent model system for study of complex epigenetic regulation of gene expression triggered by environmental cues in eukaryotes. Vernalization-mediated epigenetic changes include the formation of chromatin loops and alterations in chromatin modifications and in expression of long noncoding RNAs (lncRNAs). Changes in the three-dimensional structure of the genome, such as formation of local chromatin loops, are increasingly recognized as important gene regulatory events in eukaryotes; however, how chromatin modifications and lncRNAs coordinate to influence gene expression through changes in the three-dimensional structure of the genome is not well understood. Here we seek to understand the mechanistic details of how the three-dimensional structure of the genome influences gene regulation in vivo using developmental changes in flowering and photomorphogenesis as phenotypical read- outs. Our overriding goal is to elucidate structural and regulatory components governing protein- and lncRNA-mediated epigenetic gene regulation in Arabidopsis through three specific aims designed to: 1) elucidate the detailed mechanisms of chromatin structure-mediated gene regulation focusing on two groups of chromatin architectural proteins that we showed coordinate gene regulation through the formation of chromatin loops, 2) characterize mechanisms underlying light signaling-mediated chromatin conformation changes, and 3) characterize genome-wide changes in chromatin structure induced by environmental stimuli. Our approach will reveal the mechanistic details of chromatin structure-based epigenetic regulation by both protein and lncRNA components during flowering and photomorphogenesis. These findings will further our understanding of the mechanism of epigenetic regulation of gene expression, which has a deep evolutionary root among eukaryotes.

蛋白质和长链非编码RNA组分对发育过渡的协调调节