Coordinated regulation of floral transition by protein and long ncRNA components

蛋白质和长 ncRNA 成分对花转变的协调调控

• 批准号: 8305289
• 负责人: Sibum Sung
• 金额: $ 28.89万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8305289
• 关键词: Address; Affect; Alleles; Animals; Antibodies; Arabidopsis; Area; Biological Assay; Biological Models; Biological Process; Cells; Chromatin; Chromatin Remodeling Factor; Code; Complex; Cues; Defect; Development; Developmental Gene; Developmental Gene Expression Regulation; Developmental Process; Drosophila genus; Environment; Epigenetic Process; Epitopes; Eukaryota; Eukaryotic Cell; Event; Exanthema; Experimental Models; Flowers; Gene Expression Regulation; Genes; Genetic; Genetic Screening; Genome; Genomics; Goals; Human; Immunoprecipitation; Knock-out; Knowledge; Lead; Life Cycle Stages; Light; Measures; Mediating; Modeling; Molecular; Nature; Outcome; Paper; Pathway interactions; Photoperiod; Plant Roots; Plants; Play; Polycomb; Proteins; RNA; Reading; Regulation; Regulator Genes; Regulatory Element; Regulatory Pathway; Repression; Role; Study models; System; Temperature; Testing; Time; Transgenic Organisms; Untranslated RNA; Validation; Work; Yeasts; carcinogenesis; chromatin remodeling; design; environmental change; human disease; interest; mutant; plant growth/development; programs; response; scaffold

DESCRIPTION (provided by applicant): Coordinated regulation of floral transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable the genome to adapt to developmental and environmental cues. Flowering is one of the major developmental commitments in the plant life cycle. Plants have evolved intricate regulatory networks to control flowering time in response to both endogenous and environmental conditions. We use the floral transition as a model system to study epigenetic regulation of gene expression in eukaryotes. Recent recognition of long noncoding RNAs (lncRNAs) in eukaryotes and growing examples of their function in various developmental programs indicate that lncRNAs play roles in developmental programs, carcinogenesis and other human diseases. Despite some well-characterized examples, mechanistic details of lncRNAs and their underlying roles in development are not well understood. Our recent study identified the first functional lncRNA in plants, which is necessary for the proper epigenetic silencing of a developmentally regulated gene, FLC. Here we seek to understand mechanistic details on the role of lncRNAs in development. Our proposal is to elucidate structural and regulatory components governing protein-lncRNA mediated epigenetic regulation of developmental genes. Our overriding goals are built around three specific aims: 1) Identify and characterize lncRNAs, 2) Study mechanisms in which protein and RNA components are coordinated to regulate developmental genes, 3) Employ genetic approaches to address the mechanisms to sense and measure the duration of environmental changes (i.e. cold), which are upstream events of protein-lncRNAs mediated regulation of developmental gene expression. Our approach to dissect the mechanistic details of lncRNA- mediated epigenetic regulation will be useful not only for understanding a key mechanism of plant development (i.e. flowering) but also for elucidating the mechanism of epigenetic regulation of gene expression which has a deep evolutionary root among eukaryotes. PUBLIC HEALTH RELEVANCE: Long noncoding RNA-mediated regulation of gene expression is increasingly recognized as one of uncharted areas to fully understand epigenetic mechanisms to control developmental fates of cells. Floral transition regulatory pathways provide a model system to study the "environmentally"-induced epigenetic switch of a developmental program. Rich knowledge on protein components as well as recently recognized long noncoding RNA components makes the floral transition a suitable model to understand epigenetic mechanism of gene regulation. Epigenetic regulation of gene expression is a major regulatory theme in eukaryotic gene regulation, including Yeast, Drosophila and Human. Thus our study is of broader scientific interest. Furthermore, the environmentally induced nature of floral transition will provide a unique opportunity to study the effect of environment on the epigenetic regulation of gene expression in developmental programs.

描述（由申请人提供）：通过蛋白质和长期非编码RNA成分的花卉转变的协调调节项目摘要表观遗传机制使基因组能够适应发育和环境提示。开花是植物生命周期中的主要发展承诺之一。植物已经发展出复杂的调节网络，以控制内源性和环境条件的开花时间。我们将花卉转变用作模型系统，以研究真核生物中基因表达的表观遗传调节。最近对真核生物中长期非编码RNA（LNCRNA）的认识及其在各种发育计划中的功能越来越多的例子表明，LNCRNA在发育计划，致癌作用和其他人类疾病中起着作用。尽管有一些良好的例子，但LNCRNA及其在开发中的潜在作用的机理细节尚不清楚。我们最近的研究确定了植物中的第一个功能性lncRNA，这对于对发育调节基因的适当表观遗传沉默是必不可少的。在这里，我们试图了解有关LNCRNA在开发中作用的机理细节。我们的建议是阐明管理蛋白质lncRNA介导的发育基因的表观遗传调节的结构和调节成分。 Our overriding goals are built around three specific aims: 1) Identify and characterize lncRNAs, 2) Study mechanisms in which protein and RNA components are coordinated to regulate developmental genes, 3) Employ genetic approaches to address the mechanisms to sense and measure the duration of environmental changes (i.e. cold), which are upstream events of protein-lncRNAs mediated regulation of developmental gene expression.我们剖析LNCRNA介导的表观遗传调节的机理细节的方法不仅有用，这不仅有助于理解植物发育的关键机制（即开花），而且还可以阐明基因表达的表观遗传调节的机制，这在真核生物之间具有深层进化。 公共卫生相关性：越来越多的无编码RNA介导的基因表达调节被认为是未知的领域之一，以完全理解控制细胞发育命运的表观遗传机制。花卉过渡调节途径提供了一个模型系统，以研究发展计划的“环境”诱导的表观遗传转换。关于蛋白质成分以及最近认识的长期非编码RNA成分的丰富知识使得花卉过渡成为了解基因调节表观遗传机理的合适模型。基因表达的表观遗传调节是真核基因调节的主要调节主题，包括酵母，果蝇和人。因此，我们的研究具有更广泛的科学利益。此外，花卉过渡的环境感应性质将为研究环境对发展程序中基因表达的表观遗传调节的影响提供独特的机会。