Coordinated regulation of floral transition by protein and long noncoding RNA components

蛋白质和长非编码RNA组分对花转换的协调调控 项目摘要 表观遗传机制使有机体能够适应发育和环境线索。开花是一种 植物生命周期中的主要发展承诺。植物进化出错综复杂的调控网络来控制 开花时间对内源和环境条件的反应。例如，长时间的感冒触发 春化，通过参与发育的基因的表观遗传变化来加速开花的过程。 因此，拟南芥的春化反应是研究复杂表观遗传的一个很好的模式系统 真核生物中环境线索触发的基因表达调控。春化作用介导的表观遗传学变化 包括染色质环的形成以及染色质修饰和Long表达的改变 非编码RNA(LncRNAs)。基因组三维结构的变化，如局部染色质的形成 环，越来越被认为是真核生物中重要的基因调控事件；然而，染色质如何 修饰和lncRNAs协同作用通过改变基因的三维结构影响基因表达 基因组还没有被很好地理解。在这里，我们试图了解染色质修饰和 IncRNAs介导基因调控。我们的首要目标是阐明结构和监管部分 蛋白-lncRNA介导拟南芥春化过程中的表观遗传基因调控 旨在：1)阐明lncRNA介导的基因调控的详细机制，重点是两个lncRNA 我们通过基因内染色质环的形成展示了参与的基因的协同抑制流动，2) 评估介导染色质修饰的蛋白质和lncRNA组分之间的配位，3)执行 在发育转变过程中发生表观遗传变化的其他基因座的功能特征。我们的 该方法将揭示lncRNA介导的表观遗传调控的机制细节，因为它发生在一个关键的过程中。 植物发育的关键时期(即开花)。这些发现将进一步加深我们对表观遗传机制的理解。 基因表达调控，这在真核生物中有着深厚的进化根源。

Coordinated regulation of floral transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable organisms to adapt to developmental and environmental cues. Flowering is one of the major developmental commitments in the plant life cycle. Plants have evolved intricate regulatory networks to control flowering time in response to both endogenous and environmental conditions. For example, prolonged cold triggers vernalization, a process that accelerates flowering through epigenetic changes in genes involved in development. Therefore, the vernalization response in Arabidopsis is an excellent model system for study of complex epigenetic regulation of gene expression triggered by environmental cues in eukaryotes. Vernalization-mediated epigenetic changes include the formation of chromatin loops and alterations in chromatin modifications and in expression of long noncoding RNAs (lncRNAs). Changes in three-dimensional structure of genome, such as formation of local chromatin loops, are increasingly recognized as important gene regulatory events in eukaryotes; however, how chromatin modifications and lncRNAs coordinate to influence gene expression through changes in three-dimensional structure of genome is not well understood. Here we seek to understand the mechanistic details of how chromatin modifications and lncRNAs mediate gene regulation. Our overriding goal is to elucidate structural and regulatory components governing protein-lncRNA mediated epigenetic gene regulation during vernalization in Arabidopsis through three specific aims designed to: 1) elucidate the detailed mechanisms of lncRNA-mediated gene regulation focusing on two lncRNAs that we showed coordinate the repression of gene involved flowing through the formation of intragenic chromatin loop, 2) evaluate coordination between protein and lncRNA components that mediate chromatin modification, 3) perform functional characterization of additional loci that undergo epigenetic changes upon developmental transition. Our approach will reveal the mechanistic details of lncRNA-mediated epigenetic regulation as it occurs during a key process of plant development (i.e., flowering). These findings will further our understanding of the mechanism of epigenetic regulation of gene expression, which has a deep evolutionary root among eukaryotes.