Coordinated regulation of floral transition by protein and long ncRNA components

蛋白质和长 ncRNA 成分对花转变的协调调控

• 批准号: 8642195
• 负责人: Sibum Sung
• 金额: $ 28.98万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8642195
• 关键词: Address; Affect; Alleles; Animals; Antibodies; Arabidopsis; Area; Biological Assay; Biological Models; Biological Process; Cells; Chromatin; Chromatin Remodeling Factor; Code; Complex; Cues; Defect; Development; Developmental Gene; Developmental Gene Expression Regulation; Developmental Process; Drosophila genus; Environment; Epigenetic Process; Epitopes; Eukaryota; Eukaryotic Cell; Event; Exanthema; Experimental Models; Flowers; Gene Expression Regulation; Genes; Genetic; Genetic Screening; Genome; Genomics; Goals; Human; Immunoprecipitation; Knock-out; Knowledge; Lead; Life Cycle Stages; Light; Measures; Mediating; Modeling; Molecular; Nature; Outcome; Paper; Pathway interactions; Photoperiod; Plant Roots; Plants; Play; Polycomb; Proteins; RNA; Reading; Regulation; Regulator Genes; Regulatory Element; Regulatory Pathway; Repression; Role; Study models; System; Temperature; Testing; Time; Transgenic Organisms; Untranslated RNA; Validation; Work; Yeasts; carcinogenesis; chromatin remodeling; deep sequencing; design; environmental change; human disease; interest; mutant; plant growth/development; programs; response; scaffold

DESCRIPTION (provided by applicant): Coordinated regulation of floral transition by protein and long noncoding RNA components Project Summary Epigenetic mechanisms enable the genome to adapt to developmental and environmental cues. Flowering is one of the major developmental commitments in the plant life cycle. Plants have evolved intricate regulatory networks to control flowering time in response to both endogenous and environmental conditions. We use the floral transition as a model system to study epigenetic regulation of gene expression in eukaryotes. Recent recognition of long noncoding RNAs (lncRNAs) in eukaryotes and growing examples of their function in various developmental programs indicate that lncRNAs play roles in developmental programs, carcinogenesis and other human diseases. Despite some well-characterized examples, mechanistic details of lncRNAs and their underlying roles in development are not well understood. Our recent study identified the first functional lncRNA in plants, which is necessary for the proper epigenetic silencing of a developmentally regulated gene, FLC. Here we seek to understand mechanistic details on the role of lncRNAs in development. Our proposal is to elucidate structural and regulatory components governing protein-lncRNA mediated epigenetic regulation of developmental genes. Our overriding goals are built around three specific aims: 1) Identify and characterize lncRNAs, 2) Study mechanisms in which protein and RNA components are coordinated to regulate developmental genes, 3) Employ genetic approaches to address the mechanisms to sense and measure the duration of environmental changes (i.e. cold), which are upstream events of protein-lncRNAs mediated regulation of developmental gene expression. Our approach to dissect the mechanistic details of lncRNA- mediated epigenetic regulation will be useful not only for understanding a key mechanism of plant development (i.e. flowering) but also for elucidating the mechanism of epigenetic regulation of gene expression which has a deep evolutionary root among eukaryotes.

描述（由申请人提供）：蛋白质和长非编码 RNA 成分对花转变的协调调节 项目摘要 表观遗传机制使基因组能够适应发育和环境线索。开花是植物生命周期中的主要发育承诺之一。植物已经进化出复杂的调节网络来响应内源和环境条件来控制开花时间。我们使用花转变作为模型系统来研究真核生物基因表达的表观遗传调控。最近对真核生物中长非编码 RNA (lncRNA) 的认识以及它们在各种发育程序中的功能的例子越来越多，表明 lncRNA 在发育程序、致癌和其他人类疾病中发挥着作用。尽管有一些特征明确的例子，但 lncRNA 的机制细节及其在发育中的潜在作用尚不清楚。我们最近的研究确定了植物中第一个功能性 lncRNA，这对于发育调控基因 FLC 的适当表观遗传沉默是必要的。在这里，我们试图了解 lncRNA 在发育中的作用的机制细节。我们的建议是阐明蛋白质-lncRNA介导的发育基因表观遗传调控的结构和调控成分。我们的首要目标围绕三个具体目标：1）识别和表征lncRNA，2）研究蛋白质和RNA成分协调调节发育基因的机制，3）采用遗传方法来解决感知和测量环境变化（即寒冷）持续时间的机制，环境变化是蛋白质-lncRNA介导的发育基因表达调节的上游事件。我们剖析lncRNA介导的表观遗传调控机制细节的方法不仅有助于理解植物发育（即开花）的关键机制，而且有助于阐明基因表达的表观遗传调控机制，该机制在真核生物中具有深厚的进化根源。