Detailed functional analysis of gamete recognition protein interactions

配子识别蛋白相互作用的详细功能分析

• 批准号: 9086388
• 负责人: WILLIE J SWANSON
• 金额: $ 37.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9086388
• 关键词: Address; Affinity; Animal Model; Animals; Binding; Biochemical; Biochemistry; Biological Models; Computing Methodologies; Cytoplasmic Granules; Egg Proteins; Evolution; Exhibits; Female; Fertilization; Genes; Genomics; Germ Cells; Glycoproteins; Health; Human; Hydrogen Bonding; Individual; Light; Mammals; Marines; Molecular; Pattern; Process; Proteins; Proteome; Proteomics; Reproduction; Reproductive Biology; Research; Research Proposals; Role; Specificity; Sperm-Ovum Interactions; Structure; Study models; System; Techniques; Testing; Vertebrates; Work; Zona Pellucida; abalone; base; egg; innovation; insight; lysin; male; novel; receptor; reproductive; sperm cell; sperm protein; zona pellucida glycoprotein

DESCRIPTION (provided by applicant): The identification of interacting male-female reproductive proteins is imperative for a molecular understanding of fertilization. Generally, the molecular descriptions have relied upon studies of male reproductive proteins. This research proposal is aimed at characterizing interacting male- female reproductive proteins in abalone, a system with for which many of the molecular details of fertilization are well established and one that serves as a model for the study of reproductive molecules and evolution of species-specific fertilization. Three specific aims relating to abalone are proposed: Aim 1. Biochemical analysis of interactions among gamete recognition proteins. Aim 2. Molecular description of VE supramolecular structure through analysis of ZP glycoprotein interactions. Aim 3. Abalone sperm proteome and functional analysis of a newly discovered novel gamete recognition protein. The research proposed is innovative in that it is highly integrative, utilizing genomic, proteomic, biochemical, and computational methods. This work will test for evidence of direct functional interactions between sperm and egg molecules. The functional specificity sperm-egg interaction will be further investigated. Together these research aims represent a significant advancement in our understanding of the molecular interactions between sperm and egg, the evolutionary dynamics of genes encoding sperm/egg proteins, and will contribute to a broader understanding of the function and evolution of reproductive genes.

描述（由申请人提供）：鉴定相互作用的雄性-雌性生殖蛋白对于了解受精的分子是必不可少的。一般来说，分子描述依赖于对男性生殖蛋白的研究。本研究计划旨在描述鲍鱼中相互作用的雄性-雌性生殖蛋白，该系统中许多受精的分子细节已经建立，并且可以作为研究生殖分子和物种特异性受精进化的模型。提出了与鲍鱼有关的三个具体目标：目标1。配子识别蛋白相互作用的生化分析。目标2。通过分析ZP糖蛋白相互作用对VE超分子结构进行分子描述。目标3。鲍鱼精子蛋白质组学及其新发现的配子识别蛋白的功能分析。这项研究的创新之处在于它是高度整合的，利用了基因组学、蛋白质组学、生物化学和计算方法。这项工作将测试精子和卵子分子之间直接功能相互作用的证据。精卵相互作用的功能特异性将进一步研究。总之，这些研究目标代表了我们对精子和卵子之间的分子相互作用，编码精子/卵子蛋白质的基因的进化动力学的理解的重大进展，并将有助于更广泛地了解生殖基因的功能和进化。